Subcritical water pretreatment for agave bagasse fractionation from tequila production and enzymatic susceptibility

Singh, Anusuiya; Rodríguez-Jasso, Rosa M.; Saxena, Rohit; Belmares, Ruth; Singhania, Reeta Rani; Ruíz, Héctor A.

doi:10.1016/j.biortech.2021.125536

Cited by 24 publications

(15 citation statements)

References 31 publications

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“…Similarly, hydrothermal pretreatment involves the treatment of biomasses at elevated temperature (100-250 • C) in presence of liquid water, steam, and heat for a short treatment time and is considered eco-friendly, low cost and economically viable process. It is known that at high temperatures, water acts as catalytic media and liberates hydronium ions leading to polymerization of polysaccharides through selective hydrolysis of ether bonds and cleavage of acetyl groups [34,35]. Alternatively, alkali pretreatment involves the treatment of biomass with sodium, potassium, calcium, and ammonium hydroxide in varying concentrations leading to the breaking of ester linkages with lignin cellulose causing cellulose swelling, and partially recrystallize cellulose and partially solubilizing hemicellulose [49].…”

Section: Pretreatment Of the Cellulosic Biomassmentioning

confidence: 99%

Advances and Challenges in Biocatalysts Application for High Solid-Loading of Biomass for 2nd Generation Bio-Ethanol Production

et al. 2022

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Growth in population and thereby increased industrialization to meet its requirement, has elevated significantly the demand for energy resources. Depletion of fossil fuel and environmental sustainability issues encouraged the exploration of alternative renewable eco-friendly fuel resources. Among major alternative fuels, bio-ethanol produced from lignocellulosic biomass is the most popular one. Lignocellulosic biomass is the most abundant renewable resource which is ubiquitous on our planet. All the plant biomass is lignocellulosic which is composed of cellulose, hemicellulose and lignin, intricately linked to each other. Filamentous fungi are known to secrete a plethora of biomass hydrolyzing enzymes. Mostly these enzymes are inducible, hence the fungi secrete them economically which causes challenges in their hyperproduction. Biomass’s complicated structure also throws challenges for which pre-treatments of biomass are necessary to make the biomass amorphous to be accessible for the enzymes to act on it. The enzymatic hydrolysis of biomass is the most sustainable way for fermentable sugar generation to convert into ethanol. To have sufficient ethanol concentration in the broth for efficient distillation, high solid loading ~<20% of biomass is desirable and is the crux of the whole technology. High solid loading offers several benefits including a high concentration of sugars in broth, low equipment sizing, saving cost on infrastructure, etc. Along with the benefits, several challenges also emerged simultaneously, like issues of mass transfer, low reaction rate due to water constrains in, high inhibitor concentration, non-productive binding of enzyme lignin, etc. This article will give an insight into the challenges for cellulase action on cellulosic biomass at a high solid loading of biomass and its probable solutions.

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Section: Pretreatment Of the Cellulosic Biomassmentioning

confidence: 99%

Advances and Challenges in Biocatalysts Application for High Solid-Loading of Biomass for 2nd Generation Bio-Ethanol Production

et al. 2022

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“…In order to make the conversion processes easier, a number of different pretreatment strategies are demonstrated for the removal of substantial portions of lignin from the biomass [5]. In view of that, it was observed that few of the techniques, employing acid and hot water pretreatment significantly, disrupts the biomass network with an insignificant removal of the lignin content [6][7][8]. So, an in-depth understanding on the ultrastructural complexity of the biomass is still unclear.…”

Section: Introductionmentioning

confidence: 99%

A Critical Review on the Effect of Lignin Redeposition on Biomass in Controlling the Process of Enzymatic Hydrolysis

et al. 2022

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The key factor in lignocellulosic biorefinery is to extract maximum sugars from biomass as feedstock for the process of fermentation. The major barrier in extracting the sugars is the presence of lignin of biomass that primarily restricts the accessibility of cellulose to enzymes. Therefore, it is important to locate the presence and morphology of lignin to assess the reaction severity. In most of the severe pretreatment conditions, lignin is observed to condense and form spherical droplets on the surface of treated biomass. Such formation of pseudo-lignin differs in the action of cellulose hydrolysis from that of the initial or residual lignin content of biomass. In view of that, the present review is focused on the impact of various pretreatment techniques on the morphological changes in lignin and its subsequent redeposition or redistribution effect towards enzymatic hydrolysis process. Initially, the effect of different pretreatment methods is assessed which primarily resulted into the occurrence of lignin redeposition on the surface and interior of biomass. Thereafter, an in-depth insight is provided to understand the underlying mechanism of such formations of pseudo-lignin under severe reaction conditions. Finally, the study is concluded discussing the future recommendation to circumvent the interference of pseudo-lignin on enzymatic reactions. Hence, the present review article will not only help the readers to gain the knowledge on the formations and problems of pseudo-lignin obtained from biomass but also details the latest advancements that are involved to overcome the inhibitory effect of pseudo-lignin during the enzymatic hydrolysis of biomass. Highlights• The ultrastructural morphology of pseudo-lignin in the form of droplets are discussed.• The various pretreatment processes that are responsible for the formation of pseudo-lignin are described.• An insight on the reaction mechanisms is provided for the impact of pseudo-lignin towards enzymatic hydrolysis.• Advances processes to overcome the inhibitory effect are comprehensively summarized.• Associated challenges and future recommendations are demonstrated.

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“…In addition to being known as hot water pretreatment, hot compressed water pretreatment, autohydrolysis, or subcritical water pretreatment, hydrothermal pretreatment offers several benefits for green chemistry as a green solvent because water is used to perform the reaction at temperatures ranging from 150-220 • C and pressures ranging from 0.5 to 1.96 MPa [18]. At these critical temperatures and pressures, water begins to act like an acid [1,16,19]. The severity factor is an equation in which time and temperature interrelationships are calculated in a single variable to study the effect of changing operational conditions in hydrothermal pretreatment on biomass [19].…”

Section: Introductionmentioning

confidence: 99%

Subcritical Water Pretreatment for the Efficient Valorization of Sorghum Distillery Residue for the Biorefinery Platform

et al. 2022

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The depletion of fossil fuels is resulting in an increased energy crisis, which is leading the paradigm shift towards alternative energy resources to overcome the issue. Lignocellulosic biomass or agricultural residue could be utilized to produce energy fuel (bioethanol) as it can resolve the issue of energy crisis and reduce environmental pollution that occurs due to waste generation from agriculture and food industries. A huge amount of sorghum distillery residue (SDR) is produced during the Kaoliang liquor production process, which may cause environmental problems. Therefore, the SDR generated can be utilized to produce bioethanol to meet current energy demands and resolve environmental problems. Using a central composite experimental design, the SDR was subjected to hydrothermal pretreatment. The conditions selected for hydrothermal pretreatment are 155 °C, 170 °C, and 185 °C for 10, 30, and 50 min, respectively. Based on the analysis, 150 °C for 30 min conditions for SDR hydrothermal pretreatment were selected as no dehydration product (Furfural and HMF) was detected in the liquid phase. Therefore, the pretreated slurry obtained using hydrothermal pretreatment at 150 °C for 30 min was subjected to enzymatic hydrolysis at 5% solid loading and 15 FPU/gds. The saccharification yield obtained at 72 h was 75.05 ± 0.5%, and 5.33 g/L glucose concentration. This non-conventional way of enzymatic hydrolysis eliminates the separation and detoxification process, favoring the concept of an economical and easy operational strategy in terms of biorefinery.

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Subcritical water pretreatment for agave bagasse fractionation from tequila production and enzymatic susceptibility

Cited by 24 publications

References 31 publications

Advances and Challenges in Biocatalysts Application for High Solid-Loading of Biomass for 2nd Generation Bio-Ethanol Production

Advances and Challenges in Biocatalysts Application for High Solid-Loading of Biomass for 2nd Generation Bio-Ethanol Production

A Critical Review on the Effect of Lignin Redeposition on Biomass in Controlling the Process of Enzymatic Hydrolysis

Subcritical Water Pretreatment for the Efficient Valorization of Sorghum Distillery Residue for the Biorefinery Platform

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