Subclinical myopathy in patients affected with newly diagnosed colorectal cancer at clinical onset of disease: evidence from skeletal muscle biopsies

Abstract

Factors and mechanisms of this cancer-associated myopathy are yet unknown. The facts that the great majority of the abnormally nucleated myofibers are of the fast type and that regenerating myofibers are present, suggest a myogenic response to the colorectal cancer and not to the laparoscopic modalities of the biopsy harvesting. Follow-up of the patients will elucidate the clinical relevance of our observation, and further studies investigating the molecular mechanism underlying this early cancer-associated myopathy will hopefully provide some pathogenetic clues leading to the identification of potential specific targets for therapeutic intervention to prevent tumor cachexia.

Muscle satellite cell content and mRNA signaling in germ cell cancer patients – effects of chemotherapy and resistance training

“…Attention to cancer patients' muscular profile has traditionally been confined to the wasting syndrome of cancer cachexia [12], with several reports showing molecular changes associated with tumor-induced subclinical myopathy in muscle biopsies sampled during cancer surgery [13,14]. However, present knowledge of the molecular effects of chemotherapy on skeletal muscle in cancer patients without cachexic features is very limited [15].…”

Electrical Stimulation Counteracts Muscle Decline in Seniors

“…Muscle sections were incubated either for 1 h at room temperature (RT) or overnight at 4°C, with anti-neural adhesion molecule (N-CAM) rabbit polyclonal antibody (Chemicon, Italy), anti-Pax7 mouse monoclonal antibody (DSHB, Iowa), or anti-laminin rabbit polyclonal antibody (Sigma, Italy) 1:100 diluted in PBS, respectively, as described (Zampieri et al, 2010; Mosole et al, 2014). Sections were then incubated for 1 h at RT with Cy3 or Alexa Fluor ® 488 dye conjugated antibodies against rabbit (Chemicon, Italy) or mouse IgG (Life technologies, Italy).…”

The Need for a Consensus on the Locution “Central Nuclei” in Striated Muscle Myopathies

“…More recently, users of the “central nuclei” option include renowned researchers in the muscle field in several continents, including North America, Australia, and Europe (McGeachie and Grounds, 1999; McClung et al, 2006; Zampieri et al, 2010a; Pichavant and Pavlath, 2014). Anecdotally speaking, it seems that “centrally located nuclei” is favored by non-English speaker groups, mostly scattered through Europe or East Asia, even though “central nuclei” remains prevalent (Musarò et al, 2007; Coletti et al, 2013; Ikutomo et al, 2014).…”