Subclinical hemolytic disease of the newborn associated with IgG anti‐ Lub

Dube, Volker E.; Zoes, Caroline

doi:10.1046/j.1537-2995.1982.22382224954.x

Cited by 7 publications

(1 citation statement)

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“…The Lu a /Lu b (LU1/LU2) blood group antigen polymorphism is determined by an SNP resulting in the presence of histidine (H) or arginine (R), respectively, at amino acid 77 of the first predicted N‐terminal IgSF domain (El Nemer et al , 1997). Lutheran blood group system antibodies have been reported to be involved in mild delayed haemolytic transfusion reactions (Daniels, 2002) but are rarely involved in HDFN (Inderbitzen et al , 1982) and are not generally considered to be clinically important (Dube & Zoes, 1982; Boulton, 1990). We have studied the recombinant Lutheran protein here as an example of a single‐pass type I membrane protein.…”

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Production of soluble recombinant proteins with Kell, Duffy and Lutheran blood group antigen activity, and their use in screening human sera for Kell, Duffy and Lutheran antibodies

Ridgwell

Dixey

Scott

2007

Transfusion Medicine

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The aim of this study was to show that soluble recombinant (sr) proteins can mimic blood group antigens and be used to screen human sera for blood-group-specific antibodies. The blood of all pregnant women and pretransfusion patients should be screened for blood-group-specific antibodies to identify and monitor pregnancies at risk of haemolytic disease of the foetus and newborn (HDFN), and to prevent haemolytic transfusion reactions. Current antibody screening and identification methods use human red blood cell panels, which can complicate antibody identification if more than one antibody specificity is present. COS-7 cells were transfected to produce sr forms of the extracellular domains of the red blood cell membrane proteins that express Kell, Duffy or Lutheran blood group antigens. These sr proteins were used to screen for and identify anti-Kell, anti-Duffy or anti-Lutheran blood-group-specific allo-antibodies in human sera by haemagglutination inhibition and in solid-phase enzyme-linked immunosorbent assays (ELISAs). There is a positive correlation (correlation coefficient 0.605, P value 0.002) between antibody titre by standard indirect antiglobulin test (IAT) and signal intensity in the ELISA test. This work shows that sr proteins can mimic blood group antigens and react with human allogeneic antibodies, and that such proteins could be used to develop solid-phase, high-throughput blood group antibody screening and identification platforms.

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confidence: 99%