“…So, non-clinical local tolerance testing of gargle products is necessary to investigate the oral mucosal irritation potential. The evaluation of oral mucosal irritation has been widely conducted by methods such as contacting and swabbing of the test substances on the mucosa of cheek pouch in hamsters (Namiki et al, 1998;Anand et al, 2003;Piret et al, 2008). Other animals such as rats (Nakamura and Masuda, 1994;Arvidsson et al, 2001), guinea pigs (Nakamura and Masuda, 1994;Manabe et al, 2001), dogs (Nixon et al, 1972) and rabbits (Müller et al, 1983) have also been used for the evaluation of oral mucosal irritation.…”