Subcellular Singlet Oxygen and Cell Death: Location Matters

Liang, Pingping; Kolodieznyi, Dmytro; Creeger, Yehuda; Ballou, Byron; Bruchez, Marcel P.

doi:10.3389/fchem.2020.592941

Cited by 21 publications

(20 citation statements)

References 64 publications

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“…As 1 O 2 is an extremely short-lived molecule, and is highly reactive towards electron-rich biomolecules such as unsaturated fatty acids, the effects of the different treatments are restricted to the locality where they are generated. A recent study using chemooptogenetic methods to target photosensitisers to specific subcellular compartments highlighted the differential apoptotic and necrotic outcomes of 1 O 2 localization ( Liang et al., 2020 ). In Arabidopsis, we previously showed that RB and AO localizes 1 O 2 production to the plasma membrane and vacuole respectively.…”

Section: Introductionmentioning

confidence: 99%

Plastid and cytoplasmic origins of 1O2-mediated transcriptomic responses

2022

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The reactive oxygen species singlet oxygen, 1O2, has an extremely short half-life, yet is intimately involved with stress signalling in the cell. We previously showed that the effects of 1O2 on the transcriptome are highly correlated with 80S ribosomal arrest due to oxidation of guanosine residues in mRNA. Here, we show that dysregulation of chlorophyll biosynthesis in the flu mutant or through feeding by δ-aminolevulinic acid can lead to accumulation of photoactive chlorophyll intermediates in the cytoplasm, which generates 1O2 upon exposure to light and causes the oxidation of RNA, eliciting 1O2-responsive genes. In contrast, transcriptomes derived from DCMU treatment, or the Ch1 mutant under moderate light conditions display commonalties with each other but do not induce 1O2 gene signatures. Comparing 1O2 related transcriptomes to an index transcriptome induced by cycloheximide inhibition enables distinction between 1O2 of cytosolic or of plastid origin. These comparisons provide biological insight to cases of mutants or environmental conditions that produce 1O2.

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Section: Introductionmentioning

confidence: 99%

Plastid and cytoplasmic origins of 1O2-mediated transcriptomic responses

2022

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“…In this paper, we show that irradiation of fluorescein solutions leads to the production of 1 O 2 and CO, which affects cell viability, metabolism, and proliferation. Incubation of cells with either fluorescein or FDA enabled us to observe the biological consequences of the extra-or intracellular release of fluorescein photoproducts, especially 1 O 2 , whose short half-life limits its reactivity to the vicinity of its formation [53]. On the other hand, the long half-life of CO allows the molecule to freely pass through biological membranes, to reach dynamic equilibrium, and to affect cells regardless of its production site [44].…”

Section: Discussionmentioning

confidence: 99%

Antiproliferative and Cytotoxic Activities of Fluorescein—A Diagnostic Angiography Dye

Šranková

Dvořák

Martínek

et al. 2022

IJMS

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Fluorescein is a fluorescent dye used as a diagnostic tool in various fields of medicine. Although fluorescein itself possesses low toxicity, after photoactivation, it releases potentially toxic molecules, such as singlet oxygen (1O2) and, as we demonstrate in this work, also carbon monoxide (CO). As both of these molecules can affect physiological processes, the main aim of this study was to explore the potential biological impacts of fluorescein photochemistry. In our in vitro study in a human hepatoblastoma HepG2 cell line, we explored the possible effects on cell viability, cellular energy metabolism, and the cell cycle. We observed markedly lowered cell viability (≈30%, 75–2400 μM) upon irradiation of intracellular fluorescein and proved that this decrease in viability was dependent on the cellular oxygen concentration. We also detected a significantly decreased concentration of Krebs cycle metabolites (lactate and citrate < 30%; 2-hydroxyglutarate and 2-oxoglutarate < 10%) as well as cell cycle arrest (decrease in the G2 phase of 18%). These observations suggest that this photochemical reaction could have important biological consequences and may account for some adverse reactions observed in fluorescein-treated patients. Additionally, the biological activities of both 1O2 and CO might have considerable therapeutic potential, particularly in the treatment of cancer.

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“…These complexes have received a great deal of focus in the past decades for their application both as diagnostic and therapeutic agents. The latter application can exploit photoactivated ligand exchange reactions as well as photo-generation of toxic reactive oxygen species such as 1 O 2 within cells [84]. A second class of metal-based luminophores is represented by complexes of Pt(II), Au(III) and Au(I) [85][86][87][88][89].…”

Section: Discussionmentioning

confidence: 99%

Luminescent Metal Complexes as Emerging Tools for Lipid Imaging

et al. 2022

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Fluorescence microscopy is a key tool in the biological sciences, which finds use as a routine laboratory technique (e.g., epifluorescence microscope) or more advanced confocal, two-photon, and super-resolution applications. Through continued developments in microscopy, and other analytical methods, the importance of lipids as constituents of subcellular organelles, signalling or regulating molecules continues to emerge. The increasing recognition of the importance of lipids to fundamental cell biology (in health and disease) has prompted the development of protocols and techniques to image the distribution of lipids in cells and tissues. A diverse suite of spectroscopic and microscopy tools are continuously being developed and explored to add to the “toolbox” to study lipid biology. A relatively recent breakthrough in this field has been the development and subsequent application of metal-based luminescent complexes for imaging lipids in biological systems. These metal-based compounds appear to offer advantages with respect to their tunability of the photophysical properties, in addition to capabilities centred around selectively targeting specific lipid structures or classes of lipids. The presence of the metal centre also opens the path to alternative imaging modalities that might not be applicable to traditional organic fluorophores. This review examines the current progress and developments in metal-based luminescent complexes to study lipids, in addition to exploring potential new avenues and challenges for the field to take.

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Subcellular Singlet Oxygen and Cell Death: Location Matters

Cited by 21 publications

References 64 publications

Plastid and cytoplasmic origins of 1O2-mediated transcriptomic responses

Plastid and cytoplasmic origins of 1O2-mediated transcriptomic responses

Antiproliferative and Cytotoxic Activities of Fluorescein—A Diagnostic Angiography Dye

Luminescent Metal Complexes as Emerging Tools for Lipid Imaging

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