Subcellular plasticity of the corticotropin-releasing factor receptor in dendrites of the mouse bed nucleus of the stria terminalis following chronic opiate exposure

Jaferi, Azra; Lane, Diane; Pickel, Virginia M.

doi:10.1016/j.neuroscience.2009.06.029

Cited by 17 publications

(19 citation statements)

References 79 publications

(106 reference statements)

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“…CRFr internalization was also reported in the bed nucleus of stria terminalis following chronic morphine treatment using high resolution electron microscopy (Jaferi et al 2009). Compared to saline, mice that received chronic morphine showed a significantly lower plasmalemma and greater cytoplasmic density of the CRFr immunogold particles.…”

Section: Using High Resolution Immuno-electron Microscopy For Examinimentioning

confidence: 84%

“…Neuroanatomical techniques used to discern the cellular distribution of GPCRs have revealed important sites of action that can inform experimental approaches using pharmacological manipulation to target these systems under different experimental conditions (Reyes et al 2006, 2008; Jaferi et al 2009; Treweek et al 2009; Bangasser et al 2010, 2013; Williams et al 2011). However, light and fluorescence microscopy techniques may provide only limited resolution.…”

Section: Using High Resolution Immuno-electron Microscopy For Examinimentioning

confidence: 99%

“…Triton X-100) can be considered (Leranth and Pickel 1989). Techniques used to discern the cellular distribution of GPCRs have highlighted the importance of trafficking of these receptors under different conditions (Reyes et al 2006, 2008; Jaferi et al 2009; Treweek et al 2009; Bangasser et al 2010, 2013; Williams et al, 2011). In summary, under carefully controlled conditions, dual peroxidase and gold immunocytochemistry can enable a quantitative evaluation of the co-localization of neurotransmitters and receptors within the neuropil.…”

Section: Using High Resolution Immuno-electron Microscopy For Examinimentioning

confidence: 99%

“…Compared to saline, mice that received chronic morphine showed a significantly lower plasmalemma and greater cytoplasmic density of the CRFr immunogold particles. Interestingly, within the cytoplasmic compartment of somata and dendrites, the proportion of CRFr immunogold particles associated with mitochondria was three times as great in mice receiving morphine compared with saline (Jaferi et al 2009). This subcellular distribution is consistent with morphine-, and CRFr-associated modulation of intracellular calcium release or oxidative stress (Pickel et al 1997; Trushina and McMurray 2007).…”

Section: Using High Resolution Immuno-electron Microscopy For Examinimentioning

confidence: 99%

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Using high resolution imaging to determine trafficking of corticotropin-releasing factor receptors in noradrenergic neurons of the rat locus coeruleus

Reyes¹,

Bangasser²,

Valentino³

et al. 2014

Life Sciences

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Trafficking of G protein-coupled receptors (GPCRs) is a critical determinant of cellular sensitivity of neurons. To understand how endogenous or exogenous ligands impact cell surface expression of GPCRs, it is essential to employ approaches that achieve superior anatomical resolution at the synaptic level. In situations in which light and fluorescence microscopy techniques may provide only limited resolution, electron microscopy provides enhanced subcellular precision. Dual labeling immunohistochemistry employing visually distinct immunoperoxidase and immunogold markers has been an effective approach for elucidating complex receptor profiles at the synapse and to definitively establish the localization of individual receptors and neuromodulators to common cellular profiles. The immuno-electron microscopy approach offers the potential for determining membrane versus intracellular protein localization, as well as the association with various identifiable cellular organelles. Corticotropin-releasing factor (CRF) is an important regulator of endocrine, autonomic, immunological, behavioral and cognitive limbs of the stress response. Dysfunction of this neuropeptide system has been associated with several psychiatric disorders. This review summarizes findings from neuroanatomical studies, with superior spatial resolution, that indicate that the distribution of CRF receptors is a highly dynamic process that, in addition to being sexually dimorphic, involves complex regulation of receptor trafficking within extrasynaptic sites that have significant consequences for adaptations to stress, particularly within the locus coeruleus (LC), the major brain norepinephrine-containing nucleus.

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Section: Using High Resolution Immuno-electron Microscopy For Examinimentioning

confidence: 84%

Section: Using High Resolution Immuno-electron Microscopy For Examinimentioning

confidence: 99%

Section: Using High Resolution Immuno-electron Microscopy For Examinimentioning

confidence: 99%

Section: Using High Resolution Immuno-electron Microscopy For Examinimentioning

confidence: 99%

See 2 more Smart Citations

Using high resolution imaging to determine trafficking of corticotropin-releasing factor receptors in noradrenergic neurons of the rat locus coeruleus

Reyes¹,

Bangasser²,

Valentino³

et al. 2014

Life Sciences

View full text Add to dashboard Cite

show abstract

“…CRF receptors have been identified on mitochondria, but CRF trafficking to the mitochondria and CRF direct effects at the mitochondria have not been shown (27).…”

Section: Additional Peptides/proteins Of Interestmentioning

confidence: 99%

The mitochondrial component of intracrine action

Ré

Cook

2010

American Journal of Physiology-Heart and Circulatory Physiology

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In recent years the actions of intracellular-acting, extracellular signaling proteins/peptides (intracrines) have become increasingly defined. General principles of intracrine action have been proposed. Mitochondria represent one locus of intracrine action, and thus far, angiotensin II, transforming growth factor-β, growth hormone, atrial natriuretic peptide, Wnt 13, stanniocalcin, other renin-angiotensin system components, and vascular endothelial-derived growth factor, among others, have been shown to be mitochondria-localizing intracrines. The implications of this mitochondrial intracrine biology are discussed.

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Enhanced dendritic availability of μ‐opioid receptors in inhibitory neurons of the extended amygdala in mice deficient in the corticotropin‐releasing factor‐1 receptor

Jaferi

Zhou

Pickel

2010

Synapse

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Activation of the corticotropin-releasing factor-1 (CRF-1) receptor in the anterolateral BNST (BSTal), a key subdivision of the extended amygdala, elicits opiate-seeking behavior exacerbated by stress. However, it is unknown whether the presence of CRF-1 affects expression of the muopioid receptor (μ-OR) in the many GABAergic BSTal neurons implicated in the stress response. We hypothesized that deletion of the CRF-1 receptor gene would alter the density and/or subcellular distribution of μ-ORs in GABAergic neurons of the BSTal. We used electron microscopy to quantitatively examine μ-OR immunogold and GABA immunoperoxidase labeling in the BSTal of CRFr-1 knockout (KO) compared to wildtype (WT) mice. To assess regional specificity, we examined μ-OR distribution in dorsal striatum. The μ-ORs in each region were predominantly localized in dendrites, many of which were GABA-immunoreactive. Significantly more cytoplasmic μ-OR gold particles per dendritic area were observed selectively in GABAcontaining dendrites of the BSTal, but not of the dorsal striatum, in KO compared to WT mice. In both regions, however, significantly fewer GABA-immunoreactive axon terminals were present in KO compared to WT mice. Our results suggest that absence of CRF-1 results in enhanced expression and/or dendritic trafficking of μ-ORs in inhibitory BSTal neurons. They also suggest that expression of CRF-1 is a critical determinant of the availability of GABA in functionally diverse brain regions. These findings underscore the complex interplay between CRF, opioid and GABA systems in limbic and striatal regions, and have implications for the role of CRF-1 in influencing the pharmacological effects of opiates active at μ-ORs.

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Subcellular plasticity of the corticotropin-releasing factor receptor in dendrites of the mouse bed nucleus of the stria terminalis following chronic opiate exposure

Cited by 17 publications

References 79 publications

Using high resolution imaging to determine trafficking of corticotropin-releasing factor receptors in noradrenergic neurons of the rat locus coeruleus

Using high resolution imaging to determine trafficking of corticotropin-releasing factor receptors in noradrenergic neurons of the rat locus coeruleus

The mitochondrial component of intracrine action

Enhanced dendritic availability of μ‐opioid receptors in inhibitory neurons of the extended amygdala in mice deficient in the corticotropin‐releasing factor‐1 receptor

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