Subcellular metal distributions and metallothionein associations in rough-toothed dolphins (Steno bredanensis) from Southeastern Brazil

Monteiro, Fernanda de Souza; Lemos, Leila S.; Moura, Jailson Fulgêncio de; Rocha, Rafael Christian Chávez; Moreira, Isabel; Beneditto, Ana Paula Madeira Di; Kehrig, Helena A.; Bordon, Isabella C.; Siciliano, Salvatore; Saint’Pierre, Tatiana Dillenburg; Hauser‐Davis, Rachel Ann

doi:10.1016/j.marpolbul.2019.06.038

Cited by 19 publications

(13 citation statements)

References 63 publications

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“…Previous studies have showed synthesis of MTs and GSH in response to exposure of an aquatic animal to toxic elements ( Lavradas et al, 2014 ; Souza et al, 2018 ). Biomarker synthesis was observed in liver, muscle, and kidney tissues that work on the capture, storage, and excretion of toxic elements in a variety of aquatic organisms including mussels, crabs, fish, and marine mammals ( Lavradas et al, 2014 , 2016 ; Souza et al, 2018 ; Monteiro et al, 2019 ). It is noteworthy that similar effects were observed in contaminated freshwater ecosystems in the Rio Doce basin following the Samarco disaster, with fish exhibiting an induction of the proteins and enzymes expression related to contamination and hepatic damage ( Weber et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Contamination and oxidative stress biomarkers in estuarine fish following a mine tailing disaster

Gabriel

Hauser‐Davis

Soares

et al. 2020

PeerJ

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Background The Rio Doce estuary, in Brazil, was impacted by the deposition of iron mine tailings, caused by the collapse of a dam in 2015. Based on published baseline datasets, the estuary has been experiencing chronic trace metal contamination effects since 2017, with potential bioaccumulation in fishes and human health risks. As metal and metalloid concentrations in aquatic ecosystems pose severe threats to the aquatic biota, we hypothesized that the trace metals in estuarine sediments nearly two years after the disaster would lead to bioaccumulation in demersal fishes and result in the biosynthesis of metal-responsive proteins. Methods We measured As, Cd, Cr, Cu, Fe, Mn, Pb, Se and Zn concentrations in sediment samples in August 2017 and compared to published baseline levels. Also, trace metals (As, Cd, Cr, Cu, Fe, Hg, Mn, Pb, Se and Zn) and protein (metallothionein and reduced glutathione) concentrations were quantified in the liver and muscle tissues of five fish species (Cathorops spixii, Genidens genidens, Eugerres brasilianus, Diapterus rhombeus and Mugil sp.) from the estuary, commonly used as food sources by local populations. Results Our results revealed high trace metal concentrations in estuarine sediments, when compared to published baseline values for the same estuary. The demersal fish species C. spixii and G. genidens had the highest concentrations of As, Cr, Mn, Hg, and Se in both, hepatic and muscle, tissues. Trace metal bioaccumulation in fish was correlated with the biosynthesis of metallothionein and reduced glutathione in both, liver and muscle, tissues, suggesting active physiological responses to contamination sources. The trace metal concentrations determined in fish tissues were also present in the estuarine sediments at the time of this study. Some elements had concentrations above the maximum permissible limits for human consumption in fish muscles (e.g., As, Cr, Mn, Se and Zn), suggesting potential human health risks that require further studies. Our study supports the high biogeochemical mobility of toxic elements between sediments and the bottom-dwelling biota in estuarine ecosystems.

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Section: Introductionmentioning

confidence: 99%

Contamination and oxidative stress biomarkers in estuarine fish following a mine tailing disaster

Gabriel

Hauser‐Davis

Soares

et al. 2020

PeerJ

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“…For example, the study carried out by Decataldo et al (2004) analyzed 13 Stenella coeruleoalba and Tursiops truncatus specimens stranded along the southern coasts of Italy from 1991 to 1999 and reported the presence of Cd in all three subcellular fractions. In another study, Cd was found in both the insoluble fraction and the heat-denaturable fraction in Steno bredanensis specimens sampled off the coast of Rio de Janeiro, in five livers, three kidneys and six muscle samples obtained in 2005, 2007, and 2011, with no stable pattern observed, which seems to indicate a certain amount of detoxification attempts by MT (Monteiro et al, 2019). For example, both Mediterranean Stenella coeruleoalba and Tursiops truncatus have been reported as feeding preferentially on squid, considered the main source of Cd transference to marine mammals (Bustamante et al, 1998), and mid-level carnivorous fish species that feed on crustaceans and small fish (i.e., cod and hake, respectively), (Würtz and Marrale, 1993;Blanco et al, 2001) and Steno bredanensis feed on cephalopods and both oceanic and coastal fish (Dorneles et al, 2007;Melo et al, 2010), although the species feeding ecology is still relatively poorly known (Lodi and Hetzel, 1999).…”

Section: Discussionmentioning

confidence: 95%

“…Although a feeding overlap of Guiana dolphins with Steno bredanensis in Brazil concerning certain prey items prey is noted, i.e., squid (Loligo plei and L. sanpaulensis; Di Beneditto and Siciliano, 2007;Di Beneditto et al, 2017), Guiana dolphins also feed on the toplevel carnivorous largehead hairtail fish, which usually contain high metal levels, especially mercury. However, differences in metal metabolism and metal bioavailability, leading to differential intracellular metal compartmentalization and deleterious effects (Decataldo et al, 2004;Marijić and Raspor, 2006;Monteiro et al, 2019) must also be taken into account, although no reliable systematic assessments on how differential metal levels affect the induction process of MT detoxification pathways are available. In addition, protective effects of essential metals against toxic elements are also important, such as the protective effect of Fe, Se, and Zn against Hg toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…Cetaceans are considered excellent sentinel species concerning environmental pollution (Kehrig et al, 2016), and several studies have been carried out regarding total metal and metalloid concentrations in different species worldwide. For example, trace metals have been determined in the liver and kidney of the Franciscana dolphin Pontoporia blainvillei from the northern coast of Rio de Janeiro State, Brazil (Lailson-Brito et al, 2002), in liver, kidney, brain, lung, muscle of striped dolphins (Stenella coeruleoalba) found stranded along the Murcia coastline, in the Mediterranean Sea (Martínez-López et al, 2019), in the kidney, liver, and muscle of common dolphins (Delphinus delphis) from Portugal (Monteiro et al, 2016), among many others However, almost no assessments regarding subcellular metal contents in these animals are available worldwide, and only one report is available for the southern hemisphere (Monteiro et al, 2019). It is important to note that this type of assessment is an important step to further understand the potentially toxic effects of these pollutants in marine mammals, with direct relation to humans and public health.…”

Section: Introductionmentioning

confidence: 99%

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Subcellular Cadmium, Lead and Mercury Compartmentalization in Guiana Dolphins (Sotalia guianensis) From Southeastern Brazil

et al. 2020

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Marine mammals are considered excellent ocean health sentinels and are ubiquitously exposed to chemical contaminants worldwide. The Guiana dolphin (Sotalia guianensis) is a near-threatened dolphin species from Brazil with unknown population size data. This indicates the need for assessments regarding deleterious effects that may arise from exposure to chemical contamination, especially metals. After entry in the organism, these compounds are subject to internal subcellular compartmentalization, which in turn alters their bioavailability. However, almost no assessments regarding subcellular metal contents in marine mammals are available. In this context, metal compartmentalization was determined in three subcellular fractions for three toxic elements, Cd, Hg and Pb, by inductively coupled plasma mass spectrometry (ICP-MS) in Guiana dolphin kidney and liver samples from Southeastern Brazil. Differential metal-detoxification mechanisms were observed for the three elements, where metallothionein (MT) detoxification was postulated for only for Pb, while Cd and Hg were poorly associated to MT, and mostly present in the insoluble fraction, indicating low bioavailability. This is the first report on subcellular metal compartmentalization in Guiana dolphins and indicates that critical biochemical detoxification data is obtained through subcellular fraction analyses in marine mammals. This indicates an emerging study field for this type of assessment, which may, in turn, aid in conservation efforts.

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“…These local port activities provide logistics to the oil and gas production that have been growing since then, affecting coastal areas (Zappes et al 2016). In this context, recent assessments have indicated a considerable burden of contaminants in populations of rough-toothed dolphins along the SE Brazilian coast (Monteiro et al 2019(Monteiro et al , 2020. This study presents the solitary behavior of a sociable rough-toothed dolphin in a port area on northern Rio de Janeiro State, Brazil.…”

Section: Introductionmentioning

confidence: 98%

A solitary rough-toothed dolphin (Steno bredanensis) in a superport area on northern Rio de Janeiro State, SE Brazil

Beneditto

Siciliano

2020

Biota Neotrop.

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The solitary behavior is an unusual and peculiar behavior reported in a few sociable dolphin species. This study presents the solitary behavior of an adult rough-toothed dolphin, Steno bredanensis, in the Açu Superport (21º49’S; 041º00’W), northern Rio de Janeiro State, SE Brazil. Data about the dolphin were obtained from mobile phone videos and reports by local professionals. Probably, the same dolphin was there since 2017-2018 up to April 2020 (at least). In general, the solitary dolphin swims next to supply boats navigating along the port internal channel, and it rarely continues towards open sea. The dolphin plays with buoys and containment barriers, rubs itself against hulls, and bow-rides and jumps. There is no report of people swimming with this solitary dolphin, touching it, or offering it food in the Açu Superport. Thus, there seems to be no threats to the animal’s integrity locally.

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Subcellular metal distributions and metallothionein associations in rough-toothed dolphins (Steno bredanensis) from Southeastern Brazil

Cited by 19 publications

References 63 publications

Contamination and oxidative stress biomarkers in estuarine fish following a mine tailing disaster

Contamination and oxidative stress biomarkers in estuarine fish following a mine tailing disaster

Subcellular Cadmium, Lead and Mercury Compartmentalization in Guiana Dolphins (Sotalia guianensis) From Southeastern Brazil

A solitary rough-toothed dolphin (Steno bredanensis) in a superport area on northern Rio de Janeiro State, SE Brazil

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