Subcellular localization of WD40 repeat 1 protein in PC12 rat pheochromocytoma cells

Shin, Dong Hoon; Lee, Eun‐Ju; Chung, Yoon Hee; Mun, Ga Hee; Park, Ji Yeong; Lomax, Margaret I.; Oh, Seung Ha

doi:10.1016/j.neulet.2004.06.053

Cited by 7 publications

(3 citation statements)

References 11 publications

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“…2). This polypeptide was similar in size to the 51 kDa band observed in rat pheochromocytoma cells (Shin et al, 2004). This might represent one or more versions of WDR1: an uncharacterized WDR1 isoform, alternative splicing, and/or Fig.…”

Section: Western Blotting In Chicken Musclesupporting

confidence: 57%

WDR1 presence in the songbird basilar papilla

et al. 2008

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WD40 repeat 1 protein (WDR1) was first reported in the acoustically injured chicken inner ear, and bioinformatics revealed that WDR1 has numerous WD40 repeats, important for protein-protein interactions. It has significant homology to actin interacting protein 1 (Aip1) in several lower species such as yeast, roundworm, fruitfly and frog. Several studies have shown that Aip1 binds cofilin/actin depolymerizing factor, and that these interactions are pivotal for actin disassembly via actin filament severing and actin monomer capping. However, the role of WDR1 in auditory function has yet to be determined. WDR1 is typically restricted to hair cells of the normal avian basilar papilla, but is redistributed towards supporting cells after acoustic overstimulation, suggesting that WDR1 may be involved in inner ear response to noise stress. One aim of the present study was to resolve the question as to whether stress factors, other than intense sound, could induce changes in WDR1 presence in the affected avian inner ear. Several techniques were used to assess WDR1 presence in the inner ears of songbird strains, including Belgian Waterslager (BW) canary, an avian strain with degenerative hearing loss thought to have a genetic basis. Reverse transcription, followed by polymerase chain reactions with WDR1-specific primers, confirmed WDR1 presence in the basilar papillae of adult BW, non-BW canaries, and zebra finches. Confocal microscopy examinations, following immunocytochemistry with anti-WDR1 antibody, localized WDR1 to the hair cell cytoplasm along the avian sensory epithelium. In addition, little, if any, staining by anti-WDR1 antibody was observed among supporting cells in the chicken or songbird ear. The present observations confirm and extend the early findings of WDR1 localization in hair cells, but not in supporting cells, in the normal avian basilar papilla. However, unlike supporting cells in the acoustically damaged chicken basilar papilla, the inner ear of the BW canary showed little, if any, WDR1 up-regulation in supporting cells. This may be due to the fact that the BW canary already has established hearing loss and/or to the possibility that the mechanism(s) involved in BW hearing loss may not be related to WDR1.

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Section: Western Blotting In Chicken Musclesupporting

confidence: 57%

WDR1 presence in the songbird basilar papilla

et al. 2008

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“…In accordance with our conclusion, several other WD40-containing proteins have been shown to control the dynamic distribution of their interactors. [70][71][72] The molecular mechanisms that govern the nucleocytoplasmic shuttling of Tax-1 remain unclear. It will be important to examine more closely the role of WD40 proteins in this phenomenon.…”

Section: Discussionmentioning

confidence: 99%

Human T-cell leukemia virus type-1 Tax oncoprotein regulates G-protein signaling

Twizere

Springael

Boxus

et al. 2006

Blood

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“…Once activated, the serine protease domain of tmprss2 is released from the cell surface into the extracellular space and activates PAR (protease-activated receptor)-2 that has a role in prostate cancer and tumor metastasis [ 70 ]. Among the proteins correlating with cytoskeleton dynamics, our SSH identified a transcript ( wdr1 ) encoding a 67-kDa WD40 repeat protein 1 which is the vertebrate homologue of actin-interacting protein 1 [ 71 ]. Wdr1 is involved in actin dynamics and seems to be required to induce cell morphologic changes, especially mitotic cell rounding [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

The inflammatory and normal transcriptome of mouse bladder detrusor and mucosa

et al. 2006

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BackgroundAn organ such as the bladder consists of complex, interacting set of tissues and cells. Inflammation has been implicated in every major disease of the bladder, including cancer, interstitial cystitis, and infection. However, scanty is the information about individual detrusor and urothelium transcriptomes in response to inflammation. Here, we used suppression subtractive hybridizations (SSH) to determine bladder tissue- and disease-specific genes and transcriptional regulatory elements (TRE)s. Unique TREs and genes were assembled into putative networks.ResultsIt was found that the control bladder mucosa presented regulatory elements driving genes such as myosin light chain phosphatase and calponin 1 that influence the smooth muscle phenotype. In the control detrusor network the Pax-3 TRE was significantly over-represented. During development, the Pax-3 transcription factor (TF) maintains progenitor cells in an undifferentiated state whereas, during inflammation, Pax-3 was suppressed and genes involved in neuronal development (synapsin I) were up-regulated. Therefore, during inflammation, an increased maturation of neural progenitor cells in the muscle may underlie detrusor instability. NF-κB was specifically over-represented in the inflamed mucosa regulatory network. When the inflamed detrusor was compared to control, two major pathways were found, one encoding synapsin I, a neuron-specific phosphoprotein, and the other an important apoptotic protein, siva. In response to LPS-induced inflammation, the liver X receptor was over-represented in both mucosa and detrusor regulatory networks confirming a role for this nuclear receptor in LPS-induced gene expression.ConclusionA new approach for understanding bladder muscle-urothelium interaction was developed by assembling SSH, real time PCR, and TRE analysis results into regulatory networks. Interestingly, some of the TREs and their downstream transcripts originally involved in organogenesis and oncogenesis were also activated during inflammation. The latter represents an additional link between inflammation and cancer. The regulatory networks represent key targets for development of novel drugs targeting bladder diseases.

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Subcellular localization of WD40 repeat 1 protein in PC12 rat pheochromocytoma cells

Cited by 7 publications

References 11 publications

WDR1 presence in the songbird basilar papilla

WDR1 presence in the songbird basilar papilla

Human T-cell leukemia virus type-1 Tax oncoprotein regulates G-protein signaling

The inflammatory and normal transcriptome of mouse bladder detrusor and mucosa

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