2022
DOI: 10.1038/s41467-022-33564-7
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Subcellular localization of type IV pili regulates bacterial multicellular development

Abstract: In mammals, subcellular protein localization of factors like planar cell polarity proteins is a key driver of the multicellular organization of tissues. Bacteria also form organized multicellular communities, but these patterns are largely thought to emerge from regulation of whole-cell processes like growth, motility, cell shape, and differentiation. Here we show that a unique intracellular patterning of appendages known as type IV pili (T4P) can drive multicellular development of complex bacterial communitie… Show more

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Cited by 10 publications
(13 citation statements)
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References 28 publications
(34 reference statements)
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“…ChpA and PilG are required for motility and transformability in A. baumanii (Vesel & Blokesch, 2021 ) and motility in A. baylyi (Leong et al , 2017 ). In A. baylyi , PilG localizes on a line oriented along the cell body axis, a mechanism that depends on FimL (Ellison et al , 2022 ). PilH may also function antagonistically to PilG as in P. aeruginosa (Vesel & Blokesch, 2021 ).…”
Section: Discussionmentioning
confidence: 99%
“…ChpA and PilG are required for motility and transformability in A. baumanii (Vesel & Blokesch, 2021 ) and motility in A. baylyi (Leong et al , 2017 ). In A. baylyi , PilG localizes on a line oriented along the cell body axis, a mechanism that depends on FimL (Ellison et al , 2022 ). PilH may also function antagonistically to PilG as in P. aeruginosa (Vesel & Blokesch, 2021 ).…”
Section: Discussionmentioning
confidence: 99%
“…Bacteria, in order to interact with external environmental stimuli, exhibit long proteinaceous appendages on their cell surface in a wide range of bacteria, determined as pili or fimbriae, as identified in our targeted soils [111,112]. These non-flagellar structures are classified according to their assembly pathways, being type VI pile, found predominantly in our study, whose flexible surface appendages act as virulence factors in Gram-negative bacteria, and type IV-related pili, that are also identified in Gram-positive, capable of driving environmental adaptation and fitness [113,114].…”
Section: Discussionmentioning
confidence: 99%
“…Mutants in A. baylyi were made using natural transformation as described previously (11, 19). Briefly, mutant constructs were made by splicing-by-overlap (SOE) PCR to stich (1) ∼3 kb of the homologous region upstream of the gene of interest, (2) the mutation where appropriate (for example deletion by allelic replacement with an antibiotic [AbR] cassette), and (3) ∼3 kb of the homologous downstream region.…”
Section: Methodsmentioning
confidence: 99%