Subcellular Localization of Celery Mannitol Dehydrogenase (A Cytosolic Metabolic Enzyme in Nuclei)

Yamamoto, Yuri; Zamski, E.; Williamson, John D.; Conkling, Mark A.; Pharr, D. M.

doi:10.1104/pp.115.4.1397

Cited by 19 publications

(19 citation statements)

References 29 publications

(33 reference statements)

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“…For this interaction to occur, MTD and mannitol must be co-localized relatively early in the infection process. However, fungal mannitol secreted during infection has been found in the extracellular space (e.g., Joosten et al 1990;Voegele et al 2005), while MTD in uninfected celery cells is found in the cytosol and nucleus (Zamski et al 1996;Yamamoto et al 1997). For colocalization to occur, either plant cells must take up mannitol, or MTD must be secreted in response to pathogen attack.…”

Section: Introductionmentioning

confidence: 98%

Salicylic acid stimulates secretion of the normally symplastic enzyme mannitol dehydrogenase: a possible defense against mannitol-secreting fungal pathogens

Cheng

Zamski

Guo

et al. 2009

Planta

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The sugar alcohol mannitol is an important carbohydrate with well-documented roles in both metabolism and osmoprotection in many plants and fungi. In addition to these traditionally recognized roles, mannitol is reported to be an antioxidant and as such may play a role in host-pathogen interactions. Current research suggests that pathogenic fungi can secrete mannitol into the apoplast to suppress reactive oxygen-mediated host defenses. Immunoelectron microscopy, immunoblot, and biochemical data reported here show that the normally symplastic plant enzyme, mannitol dehydrogenase (MTD), is secreted into the apoplast after treatment with the endogenous inducer of plant defense responses salicylic acid (SA). In contrast, a cytoplasmic marker protein, hexokinase, remained cytoplasmic after SA-treatment. Secreted MTD retained activity after export to the apoplast. Given that MTD converts mannitol to the sugar mannose, MTD secretion may be an important component of plant defense against mannitol-secreting fungal pathogens such as Alternaria. After SA treatment, MTD was not detected in the Golgi apparatus, and its SA-induced secretion was resistant to brefeldin A, an inhibitor of Golgi-mediated protein transport. Together with the absence of a known extracellular targeting sequence on the MTD protein, these data suggest that a plant's response to pathogen challenge may include secretion of selected defensive proteins by as yet uncharacterized, non-Golgi mechanisms.

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Section: Introductionmentioning

confidence: 98%

Salicylic acid stimulates secretion of the normally symplastic enzyme mannitol dehydrogenase: a possible defense against mannitol-secreting fungal pathogens

Cheng

Zamski

Guo

et al. 2009

Planta

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“…7). Expression of an inducible mannitol transporter in tobacco in tandem with mannitol dehydrogenase would make sense, as mannitol dehydrogenase has been localized to the cytoplasm (Yamamoto et al, 1997). As Suc and mannitol uptake had a similar localization in autoradiographs from leaf discs (Fig.…”

Section: Agmat2 Acts As a Mannitol Transporter In Yeast And Transgenimentioning

confidence: 99%

“…Jennings et al (2002) demonstrated that the constitutive expression of a celery mannitol dehydrogenase activity in tobacco led to a decreased sensitivity toward the mannitol-secreting pathogenic fungus Alternaria alternata. As mannitol dehydrogenase was demonstrated to be cytosolic (Yamamoto et al, 1997), we hypothesized that a mannitol transport activity had to be present at the same time. To test this possibility, AgMaT2-transformed and untransformed plants were challenged with A. longipes (formerly A. alternata).…”

Section: Cloning and Functional Expression Of Agmat2 In Baker's Yeastmentioning

confidence: 99%

Characterization of AgMaT2, a Plasma Membrane Mannitol Transporter from Celery, Expressed in Phloem Cells, Including Phloem Parenchyma Cells

et al. 2007

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A second mannitol transporter, AgMaT2, was identified in celery (Apium graveolens L. var. dulce), a species that synthesizes and transports mannitol. This transporter was successfully expressed in two different heterologous expression systems: baker's yeast (Saccharomyces cerevisiae) cells and tobacco (Nicotiana tabacum) plants (a non-mannitol-producing species). Data indicated that AgMaT2 works as an H 1 /mannitol cotransporter with a weak selectivity toward other polyol molecules. When expressed in tobacco, AgMaT2 decreased the sensitivity to the mannitol-secreting pathogenic fungi Alternaria longipes, suggesting a role for polyol transporters in defense mechanisms. In celery, in situ hybridization showed that AgMaT2 was expressed in the phloem of leaflets, petioles from young and mature leaves, floral stems, and roots. In the phloem of petioles and leaflets, AgMaT2, as localized with specific antibodies, was present in the plasma membrane of three ontologically related cell types: sieve elements, companion cells, and phloem parenchyma cells. These new data are discussed in relation to the physiological role of AgMaT2 in regulating mannitol fluxes in celery petioles.

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“…Mannitol is the most abundant polyol in nature, participating in the translocation and storage of metabolites and providing the plants with resistance against salinity and osmotic stress (YAMAMOTO et al, 1997), and against invasions by pathogens as well (STOOP et al, 1996). Sorbitol is known to be the product of primary photosynthesis transport in many common fruit trees (LOESCHER et al, 1995).…”

Section: R áRvore Viçosa-mg V32 N5 P815-820 2008mentioning

confidence: 99%

Boron influence on concentration of polyols and other sugars in Eucalyptus

Leite

Valle²,

Bonine³

et al. 2008

Rev. Árvore

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-Although the functions of the element Boron (B) in plants have not been sufficiently clarified, several hypotheses have been raised. Some of the functions attributed to this element are synthesis and transport of carbohydrates. To evaluate the effect of B on the synthesis of some polyols and sugars in Eucalyptus grandis and hybrids "urograndis", these species were submitted to situations of supply and restriction of B. The experiment was carried out in a greenhouse, arranged in a randomized block design in a 2 x 2 factorial scheme, with 5 replicates of each genotype. The B levels were provided in the form of nutrient solution. No significant difference was observed in the content of mannitol, sorbitol, myo-inositol and scyllo-inositol and sugars α-glucose and β -glucose between E. grandis and hybrids. Arabinose was the only one to present a higher content in E. grandis on restriction of B. The effect of the presence of B was very expressive, but regarding B supply, the plants showed significant increase in the synthesis of the compounds evaluated.Keywords: Mannitol, metabolism of sugars in plants and sorbitol. INFLUÊNCIA DO BORO NA SÍNTESE DE POLIÓIS E OUTROS AÇÚCARES EM Eucalyptus RESUMO -Embora a função do elemento Boro (B) nas plantas ainda não tenha sido bem esclarecida, várias hipóteses vêm sido levantadas. Uma das funções atribuídas a esse elemento é a síntese de carboidratos. Com o objetivo de avaliar o efeito do B sobre a síntese de alguns polióis e açúcares em

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Subcellular Localization of Celery Mannitol Dehydrogenase (A Cytosolic Metabolic Enzyme in Nuclei)

Cited by 19 publications

References 29 publications

Salicylic acid stimulates secretion of the normally symplastic enzyme mannitol dehydrogenase: a possible defense against mannitol-secreting fungal pathogens

Salicylic acid stimulates secretion of the normally symplastic enzyme mannitol dehydrogenase: a possible defense against mannitol-secreting fungal pathogens

Characterization of AgMaT2, a Plasma Membrane Mannitol Transporter from Celery, Expressed in Phloem Cells, Including Phloem Parenchyma Cells

Boron influence on concentration of polyols and other sugars in Eucalyptus

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