Subcellular localization of APMCF1 and its biological significance of expression pattern in normal and malignant human tissues

Zhang, Yaqing; Li, Qinlong; Zhu, Feng-xiang; Cui, Jihong; Li, Kainan; Li, Qing; Wang, Rui‐An; Wang, Wenyong; Yan, Wei

doi:10.1186/1756-9966-28-111

Cited by 5 publications

(7 citation statements)

References 24 publications

(23 reference statements)

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“…Tissue-specific expression of SRPRB was found in various human tumors. For example, positive staining of SRPRB was found in the liver, lung, breast, colon, stomach, esophagus and testis, exhibited a ubiquitous expression pattern while its expression was upregulated in tumor tissues compared with corresponding normal tissues ( 30 ). As another study reported, both the normal and tumor tissues of ovary were absent of SRPRB expression ( 30 ).…”

Section: Discussionmentioning

confidence: 99%

“…For example, positive staining of SRPRB was found in the liver, lung, breast, colon, stomach, esophagus and testis, exhibited a ubiquitous expression pattern while its expression was upregulated in tumor tissues compared with corresponding normal tissues ( 30 ). As another study reported, both the normal and tumor tissues of ovary were absent of SRPRB expression ( 30 ). By contrast, in the present study, we found that the expression level of SRPRB was significantly downregulated in PDAC tissues, and significantly upregulated in stromal fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

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SERP1 is a novel marker of poor prognosis in pancreatic ductal adenocarcinoma patients via anti-apoptosis and regulating SRPRB/NF-κB axis

Wu³

et al. 2017

International Journal of Oncology

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Stress associated endoplasmic reticulum protein 1 (SERP1), can cause accumulation of unfolded proteins in ER stress. However, studies on the role of SERP1 in pancreatic ductal adenocarcinoma (PDAC) are still incomplete. The present study aimed at identifying whether SERP1 acts as a potential novel prognostic marker of PDAC, and analyzed its possible mechanism. GEO database analysis showed SERP1 was significantly upregulated in PDAC tissues, and strongly associated with advanced clinical stage of PDAC patients from TCGA database. Univariate and multivariate Cox regression analysis further revealed SERP1 high expression was an independent factor for the prognosis of PDAC. Gene set enrichment analysis (GSEA) revealed that SERP1 was mainly involved in regulating cell apoptosis and nuclear factor-κB (NF-κB) signaling pathway, and downregulated SERP1 significantly promoted PANC-1 cell apoptosis. To further explore its possible mechanism, protein-protein interaction (PPI) and gene ontology (GO) analysis showed the functions of proteins interacting with SERP1 were mainly enriched in regulating cell apoptosis, and SRP receptor β subunit (SRPRB) was the core of the whole PPI network. The expression of SERP1 was negatively correlated with SRPRB expression. In vitro, downregulated SERP1 significantly increased SRPRB expression. Furthermore, upregulated SRPRB could increase cell apoptosis rate and decreased the expression level of NF-κB and the phosphorylation NF-κB. The above results indicated that SERP1 as a potential novel prognostic marker of PDAC probably via regulating cell apoptosis and NF-κB activation, which may be associated with SRPRB.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

SERP1 is a novel marker of poor prognosis in pancreatic ductal adenocarcinoma patients via anti-apoptosis and regulating SRPRB/NF-κB axis

Wu³

et al. 2017

International Journal of Oncology

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“…SRPR encodes the signal recognition particle (SRP) receptor subunit alpha (“docking protein”), which together with the SRP ensures the correct targeting of the nascent secretory proteins to the endoplasmic reticulum membrane system [Janin et al., ]. Previous study show the involvement of the beta subunit of SRPR (APMCF1) in oncogenesis [Zhang et al., ], more specifically in colon cancer development [Yan et al., ].…”

Section: Discussionmentioning

confidence: 99%

“…A good example is monoallelic loss of PTEN , the main mutated gene in endometrial cancer [Nardella et al., ] and monoallelic loss of MSH3 and MSH6 in gastrointestinal cancers of the microsatellite mutator phenotype [Ohmiya et al., ]. Most of the highly mutated genes in endometrial cancer can be functionally relevant as well, since they are associated with molecular events previously reported to be involved in the carcinogenic process [Janin et al., ; Yan et al., ; Leon et al., ; Jeong et al., ; Xiong et al., ; Zhang et al., ]. JMJD1C and SVIL , with mutation frequencies of 15% and 11%, respectively, encode proteins involved in the regulation of hormone receptors.…”

Section: Discussionmentioning

confidence: 99%

New Target Genes in Endometrial Tumors Show a Role for the Estrogen-Receptor Pathway in Microsatellite-Unstable Cancers

et al. 2014

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Microsatellite instability (MSI) in tumors results in an accumulation of mutations in (target) genes. Previous studies suggest that the profile of target genes differs according to tumor type. This paper describes the first genome-wide search for target genes for mismatch repair-deficient endometrial cancers. Genes expressed in normal endometrium containing coding repeats were analyzed for mutations in tumors. We identified 44 possible genes of which seven are highly mutated (>15%). Some candidates were also found mutated in colorectal and gastric tumors. The most frequently mutated gene, NRIP1 encoding nuclear receptor-interacting protein 1, was silenced in an endometrial tumor cell line and expression microarray experiments were performed. Silencing of NRIP1 was associated with differences in the expression of several genes in the estrogen-receptor network. Furthermore, an enrichment of genes related to cell cycle (regulation) and replication was observed. We present a new profile of target genes, some of them tissue specific, whereas others seem to play a more general role in MSI tumors. The high-mutation frequency combined with the expression data suggest, for the first time, an involvement of NRIP1 in endometrial cancer development.

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“…Paraffin-embedded tissue sections on microscopic slides were processed through a graded series of alcohols and rehydrated in distilled water. Heat-induced antigen retrieval was performed by citrate buffer (10 mmol/l concentration, pH 6.0), and standard indirect biotin-avidin immunohistochemical analysis was performed as previously described (15,16).…”

Section: Cell Cycle Gene Expression Array Analysismentioning

confidence: 99%

Apoptosis-related protein-1 acts as a tumor suppressor in cholangiocarcinoma cells by inducing cell cycle arrest via downregulation of cyclin-dependent kinase subunits

Zheng

Wang

et al. 2015

Oncology Reports

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Abstract. Cholangiocarcinoma, a malignancy arising from the biliary tract, is associated with high mortality due to the late diagnosis and lack of effective therapeutic approaches. Our knowledge of the molecular alterations during the carcinogenesis of cholangiocarcinoma is limited. Previous study suggests that apoptosis-related protein-1 (Apr-1) is involved in cancer cell proliferation and survival. In the present study, we first detected the expression pattern of Apr-1 in human cholangiocarcinoma tissues and the effects of forced Apr-1 expression on cell proliferation and cell cycle progression. Cell cycle gene array analysis was used to identify downstream molecules that were regulated by Apr-1, and their expression levels were further evaluated in human cholangiocarcinoma tissues. We showed that Apr-1 expression was downregulated in human cholangiocarcinoma tissues. Forced expression of Apr-1 inhibited cell proliferation of cholangiocarcinoma cell line QBC939 and induced G2/M phase arrest. Downregulation of cell cycle-related genes cyclin-dependent kinase (Cdk) 2, and cyclin-dependent kinase subunits (Cks) 1 and 2 was involved in Apr-1-induced cell cycle arrest. Furthermore, we found that Cdk2 and Cks1/2 expression levels were elevated in human cholangiocarcinoma tissues. Taken together, our data showed that Apr-1 plays a crucial role in cell proliferation by controlling cell cycle progression, implying a tumor-suppressor function of Apr-1 in cholangiocarcinoma carcinogenesis. Thus, the present study provides a rationale to further study the underlying mechanisms of Apr-1 downregulation in cholangiocarcinoma for exploring potential diagnostic and therapeutic targets.

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Subcellular localization of APMCF1 and its biological significance of expression pattern in normal and malignant human tissues

Cited by 5 publications

References 24 publications

SERP1 is a novel marker of poor prognosis in pancreatic ductal adenocarcinoma patients via anti-apoptosis and regulating SRPRB/NF-κB axis

SERP1 is a novel marker of poor prognosis in pancreatic ductal adenocarcinoma patients via anti-apoptosis and regulating SRPRB/NF-κB axis

New Target Genes in Endometrial Tumors Show a Role for the Estrogen-Receptor Pathway in Microsatellite-Unstable Cancers

Apoptosis-related protein-1 acts as a tumor suppressor in cholangiocarcinoma cells by inducing cell cycle arrest via downregulation of cyclin-dependent kinase subunits

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