Subcellular localization and N-glycosylation of human ABCC6, expressed in MDCKII cells

Sinkó, Emese; Iliás, Attila; Ujhelly, Olga; Homolya, László; Scheffer, George L.; Bergen, Arthur A. B.; Sarkadi, Balázs; Váradi, András

doi:10.1016/s0006-291x(03)01349-4

Cited by 34 publications

(30 citation statements)

References 24 publications

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“…In a subsequent study, MRP6 was only present in the basolateral membrane of human hepatocytes (Scheffer et al, 2002a). A basolateral orientation was also demonstrated in human and mouse kidney proximal tubules and in MRP6-transfected MDCKII epithelial cells (Beck et al, 2003;Sinko et al, 2003).…”

Section: F Mrp6mentioning

confidence: 77%

Multidrug Resistance-Associated Proteins: Expression and Function in the Central Nervous System

Dallas

Miller²,

Bendayan³

2006

Pharmacol Rev

268

207

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Section: F Mrp6mentioning

confidence: 77%

Multidrug Resistance-Associated Proteins: Expression and Function in the Central Nervous System

Dallas

Miller²,

Bendayan³

2006

Pharmacol Rev

268

207

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“…The retroviral vector, SPsLdS (38), was modified previously by us to contain a neomycin resistance cassette (39). cDNA encoding wild-type (R482) ABCG2 or ABCG2 K86M was inserted by blunt-end ligation into the EcoRI sites of the retroviral vector, thus removing the original gp91 cDNA.…”

Section: Methodsmentioning

confidence: 99%

Multidrug Transporter ABCG2 Prevents Tumor Cell Death Induced by the Epidermal Growth Factor Receptor Inhibitor Iressa (ZD1839, Gefitinib)

et al. 2005

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Iressa (ZD1839, Gefitinib), used in clinics to treat non-small cell lung cancer patients, is a tyrosine kinase receptor inhibitor that leads to specific decoupling of epidermal growth factor receptor (EGFR) signaling. Recent data indicate that Iressa is especially effective in tumors with certain EGFR mutations; however, a subset of these tumors does not respond to Iressa. In addition, certain populations have an elevated risk of side effects during Iressa treatment. The human ABCG2 (BCRP/MXR/ABCP) transporter causes cancer drug resistance by actively extruding a variety of cytotoxic drugs, and it functions physiologically to protect our tissues from xenobiotics. Importantly, ABCG2 modifies absorption, distribution, and toxicity of several pharmacologic agents. Previously, we showed that ABCG2 displays a high-affinity interaction with several tyrosine kinase receptor inhibitors, including Iressa. Here, we show that the expression of ABCG2, but not its nonfunctional mutant, protects the EGFR signalingdependent A431 tumor cells from death on exposure to Iressa. This protection is reversed by the ABCG2-specific inhibitor, Ko143. These data, reinforced with cell biology and biochemical experiments, strongly suggest that ABCG2 can actively pump Iressa. Therefore, variable expression and polymorphisms of ABCG2 may significantly modify the antitumor effect as well as the absorption and tissue distribution of Iressa. (Cancer Res 2005; 65(5): 1770-7)

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“…In mice, rats, and humans, Mrp6/MRP6 mRNA is expressed in liver and kidneys, where their proteins are detected on the basolateral membranes of hepatocytes and proximal tubules (Table 6) Belinsky and Kruh, 1999;Scheffer et al, 2002a;Maher et al, 2005bMaher et al, , 2006b. Likewise, overexpression of MRP6 in MDCK cells results in basolateral trafficking (Sinkó et al, 2003). MRP6 protein has also been found in enteroendocrine G cells of the stomach (Beck et al, 2005).…”

Section: Localization Of Efflux Mrp and Bcrp Transporters In Various mentioning

confidence: 99%

Xenobiotic, Bile Acid, and Cholesterol Transporters: Function and Regulation

2010

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Subcellular localization and N-glycosylation of human ABCC6, expressed in MDCKII cells

Cited by 34 publications

References 24 publications

Multidrug Resistance-Associated Proteins: Expression and Function in the Central Nervous System

Multidrug Resistance-Associated Proteins: Expression and Function in the Central Nervous System

Multidrug Transporter ABCG2 Prevents Tumor Cell Death Induced by the Epidermal Growth Factor Receptor Inhibitor Iressa (ZD1839, Gefitinib)

Xenobiotic, Bile Acid, and Cholesterol Transporters: Function and Regulation

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