Subcellular fractionation of stored red blood cells reveals a compartment-based protein carbonylation evolution

Large scale inkjet-printing of carbon nanotubes electrodes for antioxidant assays in blood bags

“…a model AO) and in real samples such as erythrocyte concentrates (ECs), a labile blood product widely used in transfusion medicine, donated by two healthy persons and targeted for blood donation. The latter is of high relevance in blood product research and management since erythrocytes suffer from storage lesions, during storage at 4°C, which alter lipids, proteins, metabolism, and biomechanical properties of the cells [42][43][44][45][46][47][48], resulting in potential adverse effects upon transfusion [47][48][49][50][51][52]. Erythrocytes are enucleated cells not able to synthesize proteins, which requires a solid system (like the repair or destroy box) [53,54] to protect the cells against oxidative stress.…”

Systems biology of stored blood cells: Can it help to extend the expiration date?

“…Their conclusion is that carbonylation of cytoplasmic and membrane proteins differs during the storage period, describing two spate steps in the evolution of carbonylation during RBC concentrate storage [7].…”

Red Blood Cell Microparticles: Clinical Relevance

“…However, cytoskeleton alterations allow RBCs to eliminate such markers as well as other harmful components accumulating during storage thanks to the vesiculation process [16]. Interestingly, we have recently shown that RBC microvesiculation during blood banking takes part in the elimination of oxidized proteins: RBC microvesicles spontaneously released from banked erythrocytes are progressively enriched in carbonylated proteins during storage as compared to their parent cells [24]. This observation supports the hypothesis that microvesiculation may represent a mechanism allowing the elimination of deleterious components such as neoantigens or oxidized proteins.…”