Subcellular forms of cholesterol oxidase from <i>Rhodococcus</i> sp. CIP 105 335: induction, solubilization and characterization

Elalami, Aziz; Baessler, Keith; Kong, Fansen; Sampson, Nicole S.; Kreit, Joseph

doi:10.1142/9789812837554_0150

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“…ChOx induction in Rhodococcus sp. GK1 is independent of the steroid 3β‐hydroxy‐5‐ene, because cholest‐4‐en‐3‐one was demonstrated to be the inducer [53,54]. Moreover, androstenedione or testosterone, intermediates in cholesterol catabolism (Scheme 2), completely repressed ChOx synthesis by this strain.…”

Section: Cholesterol As a Nutritional Sourcementioning

confidence: 99%

“…Although disruption of the permease genes blocks cholesterol catabolism, cholesterol uptake and catabolism are not essential for survival of R. equi in the macrophage. We note that in Rhodococcus, ChOx is either secreted and/or cell‐surface‐linked (see, for example, [54,83–85]). Thus, the conversion of cholesterol into cholest‐4‐en‐3‐one occurs outside the bacterial cell, and the supAB/mce4 transporter system in rhodococci probably transports cholest‐4‐en‐3‐one rather than cholesterol.…”

Section: Cholesterol Oxidase and Virulencementioning

confidence: 99%

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Cholesterol oxidase: physiological functions

Kreit¹,

Sampson²

2009

The FEBS Journal

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An important aspect of catalysis performed by cholesterol oxidase (3β‐hydroxysteroid oxidase) concerns the nature of its association with the lipid bilayer that contains the sterol substrate. Efficient catalytic turnover is affected by the association of the protein with the membrane as well as the solubility of the substrate in the lipid bilayer. In this review, the binding of cholesterol oxidase to the lipid bilayer, its turnover of substrates presented in different physical environments, and how these conditions affect substrate specificity, are discussed. The physiological functions of the enzyme in bacterial metabolism, pathogenesis and macrolide biosynthesis are reviewed in this context.

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Section: Cholesterol As a Nutritional Sourcementioning

confidence: 99%

Section: Cholesterol Oxidase and Virulencementioning

confidence: 99%