Subcellular distribution of lysosomal enzymes in the human endometrium. I. Normal menstrual cycle

Rosado, Adolfo; Mercado, Efraı́n; Gallegos, Alfredo J.; Wens, Ma. de los Angeles; Aznar, R

doi:10.1016/0010-7824(77)90028-2

Cited by 15 publications

(12 citation statements)

References 20 publications

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“…The temporal profiles of the acid hydrolytic en¬ zymes studied during proliferative and secretory phases of the normal menstrual cycle in rhesus monkeys are very close to those in human endo¬ metria (Wood et al 1969;Henzl et al 1972;Wynn 1977;Rosado et al 1977). Moreover, high alka¬ line phosphatase activity in monkey endometria during the immediate pre-ovulatory period lend support to the earlier observation that endome¬ trial cell proliferation under E2 dominance is associated with elevation in alkaline phosphatase levels (Edward-Hall & Brooklyn 1950;Henzl et al 1968;Elias et al 1983).…”

Section: Discussionmentioning

confidence: 75%

“…We here report that in a fertile cycle, the endometrium responds to the presence of pre-implantation stage conceptus with distinct changes in its tissue levels of hydrolases. It is being speculated that low acid hydrolases and high alkaline phophatase in prenidatory stage endometria may reflect arrest of premenstrual endometrial cell involution which otherwise is initiated by the mid-secretory stage in a non-fertile cycle (Rosado et al 1977;Cornillie et al 1985).…”

Section: Discussionmentioning

confidence: 99%

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Endometrial phosphatases, β-glucuronidase and cathepsin D during menstrual cycle and pre-implantation stages of gestation in the rhesus monkey (Macaca mulatta)

Sengupta

D²,

Paria³

et al. 1988

Acta Endocrinologica

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\g=b\-glucuronidase,cathepsin D, acid and alkaline phosphatases were studied in rhesus monkey endometrium during the menstrual cycle (day \p=n-\6 to day + 10) and pre-implantation stages (day +3 to day +6) of gestation, with day 0 considered as the day of ovulation. Acid hydrolases exhibited low levels in proliferative phase endometria followed by their gradual rise in the secretory phase of the menstrual cycle. Despite no shifts in the levels of serum progesterone and estradiol-17\g=b\, the pre-implantation period was, however, associated with distinct changes in enzyme profiles characterized by lower absolute levels (P < 0.05) of acid phosphatase and \g=b\-glucuronidase on days 3 to 6 of gestation, whereas cathepsin D activity declined significantly (P < 0.05) on days 5 and 6. Alkaline phosphatase showed a characteristic rise during the pre-ovulatory period with a gradual lowering of its level in post-ovulatory phase endometria of a non-fertile cycle; in contrast, during early gestation, alkaline phosphatase activity showed a marked elevation (P < 0.05) on days 5 and 6 of gestation. The significance of these findings is discussed.

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Endometrial phosphatases, β-glucuronidase and cathepsin D during menstrual cycle and pre-implantation stages of gestation in the rhesus monkey (Macaca mulatta)

Sengupta

D²,

Paria³

et al. 1988

Acta Endocrinologica

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“…The activity of acid phosphatase in follicles of this size, both free and membrane-bound, showed a signifi- Changes in specific activities of lysosomal enzymes in cell-free follicular fluid of growing and atretic follicles. Enzymatic activities were measured fluorometrically following the enzymatic release of 4-methyl-umbelliferone from adequate substrates following the methodology described by Rosado et al (1977) and modified by Ballesteros et al (1992). Values represent the mean Ϯ the standard deviation obtained from at least six independent experiments.…”

Section: Resultsmentioning

confidence: 99%

“…Enzymatic activities were measured fluorometrically in granulosa cell homogenates and in cell-free follicular fluid, following the enzymatic release of 4-methyl-umbelliferone from adequate substrates following the methodology described by Rosado et al (1977), and modified by Ballesteros et al (1992). Measurements were done in a Perkin-Elmer MPF-3 spectrophoto-fluorimeter.…”

Section: Methodsmentioning

confidence: 99%

Multiparametric study of atresia in ewe antral follicles: Histology, flow cytometry, internucleosomal DNA fragmentation, and lysosomal enzyme activities in granulosa cells and follicular fluid

et al. 2000

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“…The lysosomal concept of endometrial bleeding was supported by (i) the high specific activities of several acid hydrolases in this tissue (8); (ii) the increase of the nonsedimentable fraction of lysosomal enzymes during the late secretory phase (9); and (iii) the cytochemical demonstration of extracellular acid phosphatase in perimenstrual endometria (10). These observations are compatible with a role of lysosomal hydrolases, but do not prove their involvement in the ECM breakdown at menstruation.…”

mentioning

confidence: 94%

Menstrual breakdown of human endometrium can be mimicked in vitro and is selectively and reversibly blocked by inhibitors of matrix metalloproteinases.

Marbaix

Kokorine

Moulin

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

145

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The mechanisms underlying the menstrual lysis leading to shedding of the human endometrium and its accompanying bleeding are still largely unknown. In particular, whether breakdown of the endometrial fibrillar extracellular matrix that precedes bleeding depends on aspartic-, cysteine-, serine-, or metalloproteinases remains unclear. In the present study, menstrual regression of the human endometrium was mimicked in organ culture. Whereas sex steroids could preserve tissue integrity only in nonperimenstrual explants, matrix breakdown upon sex steroid deprivation was completely and reversibly inhibited at all stages of the menstrual cycle by specific inhibitors of matrix metalloproteinases, but not by inhibitors of the other classes of proteinases. Matrix metalloproteinases are thus identified as the key class of proteinases involved in the initiation of menstruation.In his pioneering studies on menstruation using intraocular endometrial transplants in Rhesus monkeys, Markee constantly observed a major shrinking of the tissue within the few days preceding menstrual bleeding (1). Bleeding, which characterizes primates and a limited number of other species, has been linked to hypoxia/reperfusion secondary to spasm or compression of their unique coiled arteries upon tissue collapse (1). Regression of the endometrium also occurs in nonbleeding mammals: in cycling rats, both endometrial wet weight and collagen content decrease during metestrus to 20% of their proestrus value (2), indicating that proteolysis of the extracellular matrix (ECM) takes part in the process (3).An extensive argyrophilic network of so-called "reticular fibers," containing both type III and type I collagen (4), but whose argyrophilic staining properties are not completely understood (5), is built up in the ECM of human endometrium during the proliferative and early secretory phases of the menstrual cycle. When progesterone concentration declines at the end of the secretory phase, this embryonic-like interstitial fibrillar matrix shows focal breakdown that develops into extensive lysis at menstruation, concomitantly with more restricted sites of basement membrane disruption around vessels and glands. The shrunken tissue remnants then undergo piecemeal shedding (reviewed in ref. 6).The frequent localization of collagen fibers inside stromal cells just before and during menstruation has been interpreted as lysosomal digestion (7). The lysosomal concept of endometrial bleeding was supported by (i) the high specific activities of several acid hydrolases in this tissue (8); (ii) the increase of the nonsedimentable fraction of lysosomal enzymes during the late secretory phase (9); and (iii) the cytochemical demonstration of extracellular acid phosphatase in perimenstrual endometria (10). These observations are compatible with a role of lysosomal hydrolases, but do not prove their involvement in the ECM breakdown at menstruation.Plasmin and plasminogen activators could also be involved. The human endometrium contains both urokinase and tissue pl...

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Subcellular distribution of lysosomal enzymes in the human endometrium. I. Normal menstrual cycle

Cited by 15 publications

References 20 publications

Endometrial phosphatases, β-glucuronidase and cathepsin D during menstrual cycle and pre-implantation stages of gestation in the rhesus monkey (Macaca mulatta)

Endometrial phosphatases, β-glucuronidase and cathepsin D during menstrual cycle and pre-implantation stages of gestation in the rhesus monkey (Macaca mulatta)

Multiparametric study of atresia in ewe antral follicles: Histology, flow cytometry, internucleosomal DNA fragmentation, and lysosomal enzyme activities in granulosa cells and follicular fluid

Menstrual breakdown of human endometrium can be mimicked in vitro and is selectively and reversibly blocked by inhibitors of matrix metalloproteinases.

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