Subcellular distribution and expression of cofilin and ezrin in human colon adenocarcinoma cell lines with different metastatic potential

Nowak, Dorota; Mazur, Antonina Joanna; Popow-Woźniak, Agnieszka; Radwańska, Agata; Mannherz, Hans Georg; Malicka-Błaszkiewicz, Maria

doi:10.4081/ejh.2010.e14

Cited by 49 publications

(54 citation statements)

References 49 publications

(64 reference statements)

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“…Perhaps the increase in F-actin content provides additional scaffolding function to the BTB during germ cell depletion. Indeed, an increase in F-to G-actin ratio is detected in human adenocarcinoma cells with an increase in invasiveness, 101 illustrating active AJ restructuring pertinent to cellular migration is associated with an increase in F-actin, consistent with results obtained from the Adjudin model.…”

Section: Btb Dynamicssupporting

confidence: 86%

Cross-Talk between Tight and Anchoring Junctions—Lesson from the Testis

Yan

Mruk

Lee

et al. 2009

Advances in Experimental Medicine and Biology

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Spermatogenesis takes place in the seminiferous tubules in adult testes such as rats, in which developing germ cells must traverse the seminiferous epithelium while spermatogonia (2n, diploid) undergo mitotic and meiotic divisions, and differentiate into elongated spermatids (1n, haploid). It is conceivable that this event involves extensive junction restructuring particularly at the blood-testis barrier (BTB, a structure that segregates the seminiferous epithelium into the basal and the adluminal compartments) that occurs at stages VII-VIII of the seminiferous epithelial cycle. As such, cross-talk between tight (TJ) and anchoring junctions [e.g., basal ectoplasmic specialization (basal ES), adherens junction (AJ), desmosome-like junction (DJ)] at the BTB must occur to coordinate the transient opening of the BTB to facilitate preleptotene spermatocyte migration. Interestingly, while there are extensively restructuring at the BTB during the epithelial cycle, the immunological barrier function of the BTB must be maintained without disruption even transiently. Recent studies using the androgen suppression and Adjudin models have shown that anchoring junction restructuring that leads to germ cell loss from the seminiferous epithelium also promotes the production of AJ (e.g., basal ES) proteins (such as N-cadherins, catenins) at the BTB site. We postulate the testis is using a similar mechanism during spermatogenesis at stage VIII of the epithelial cycle that these induced basal ES proteins, likely form a "patch" surrounding the BTB, transiently maintain the BTB integrity while TJ is "opened", such as induced by TGF-b3 or TNFa, to facilitate preleptotene spermatocyte migration. However, in other stages of the epithelial cycle other than VII and VIII when the BTB remains "closed" (for ~10 days), anchoring junctions (e.g., AJ, DJ, and apical ES) restructuring continues to facilitate germ cell movement. Interestingly, the mechanism(s) that governs this communication between TJ and anchoring junction (e.g., basal ES and AJ) in the testis has remained obscure until recently. Herein, we provide a critical review based on the recently available data regarding the cross-talk between TJ and anchoring junction to allow simultaneous maintenance of the BTB and germ cell movement across the seminiferous epithelium.

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Section: Btb Dynamicssupporting

confidence: 86%

Cross-Talk between Tight and Anchoring Junctions—Lesson from the Testis

Yan

Mruk

Lee

et al. 2009

Advances in Experimental Medicine and Biology

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“…Increases in the ratio of F:G actin has previously been implicated in a transition to a metastatic step in colon adenocarcinoma cell lines. 21 Similarly, enrichment of α-actinin at the leading edge of CRC has been shown to underpin increased cell motility. 23 Finally, expression of vimentin in CRC is also associated with poor prognosis and invasiveness.…”

Section: Discussionmentioning

confidence: 99%

“…β-actin, 21,22 α4-actinin [23][24][25] and vimentin 26,27 are all components of the cytoskeleton that have previously been implicated in cancer progression, cell migration and invasiveness. Tissue transglutaminase 2 (TG2) is a Ca 2+ regulated transamidase that cross-links proteins via the formation of ε-(-γ-glutamyl) lysine isopeptide bonds.…”

Section: Discussionmentioning

confidence: 99%

The role of Lamin A in cytoskeleton organization in colorectal cancer cells

Foster¹,

Robson²,

Simon³

et al. 2011

Nucleus

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“…Ezrin is a member of the ezrinradixin-moesin (ERM) family that is mainly involved in the link between the cytoskeleton and the plasma membrane, suggesting that ezrin is associated with HCC infiltration and metastasis. [28][29][30] Nowak et al 31 detected ezrin expression in four human colon adenocarcinoma cell lines that were characterized by different metastatic abilities, and suggested that the highest ezrin expression occurs in cells with the strongest invasive ability. Li et al 32 analysed the correlation between ezrin expression and pathological characteristics of gastric cancer.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-22 expression in hepatocellular carcinoma and its correlation with ezrin protein

Zhou

Yang-de

2013

J Int Med Res

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Objective: Expression of microRNA-22 (miR-22) and ezrin protein (a membrane-cytoskeleton linking protein) in hepatocellular carcinoma (HCC) was investigated. Methods: Specimens of HCC and paracancerous tissue (control; $5 cm away from tumour tissue) were collected from 192 patients. miR-22 expression was detected by real-time polymerase chain reaction; ezrin protein expression in tumour tissue was assessed immunohistochemically. Associations between miR-22 expression and clinicopathological features of HCC and ezrin expression were analysed. Results: miR-22 expression was lower in HCC tissue than in paracancerous tissue samples (median relative expression 0.676 versus 1.000 for control tissue). Expression of miR-22 was significantly associated with histological differentiation (relative expression 0.431 for lower grades of differentiation versus 0.918 for higher grades), and was associated with lymphatic metastasis (relative expression 0.518 if metastasis was present, 0.919 if absent). Survival time was shorter in patients with low miR-22 expression than in those with high expression (31.0 AE 2.6 versus 52.2 AE 5.1 months). There was a significant negative correlation between the expression of miR-22 and that of ezrin. Conclusions: miR-22 is downregulated in HCC and its expression is associated with the differentiation, metastasis and prognosis of the carcinoma. Ezrin is a potential regulatory protein of miR-22.

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Subcellular distribution and expression of cofilin and ezrin in human colon adenocarcinoma cell lines with different metastatic potential

Cited by 49 publications

References 49 publications

Cross-Talk between Tight and Anchoring Junctions—Lesson from the Testis

Cross-Talk between Tight and Anchoring Junctions—Lesson from the Testis

The role of Lamin A in cytoskeleton organization in colorectal cancer cells

MicroRNA-22 expression in hepatocellular carcinoma and its correlation with ezrin protein

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