Subcellular characterization of glycoproteins in the principal cells of human gallbladder

Madrid, Juan Francisco; Castells, María Teresa; Martı́nez-Menárguez, José A.; Sánchez, Manuel A. Bueno; Hernández, Francisco Velasco; Ballesta, José Antonio Camacho

doi:10.1007/bf00269544

Cited by 30 publications

(38 citation statements)

References 57 publications

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“…Because this is not the subject of this work, the method will not be described. The sections were stained according to Madrid et al (1994Madrid et al ( ,1998b.…”

Section: Immunocytochemical Labelingmentioning

confidence: 99%

Lectin-Gold Localization of Fucose Residues in Human Gastric Mucosa

Madrid

Leis

Díaz‐Flores

et al. 1998

J Histochem Cytochem.

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SUMMARYThe oligosaccharides of the mucous gastric glycoproteins are involved in the protection of the gastric mucosa and are altered in different diseases. Therefore, it is important to know their composition in health, to better determine the alterations induced by the disease. Moreover, analysis of the molecular composition of the fundic gland cells has been previously used to obtain new insights into the origin of the different cell types. The aim of the present study was the localization in the subcellular structures of the fucose residues of the oligosaccharides in human fundic glands. For this, lectin cytochemical methods were used at the light and electron microscopic levels. They were combined with enzymatic and chemical treatments to characterize the nature of the oligosaccharide chains containing the fucose residues. The presence of this carbohydrate belonging to N-or O-linked oligosaccharides has been demonstrated in the secretory granules of the surface, gastric pit, mucous neck, and transitional cells of the fundic mucosa, and in the intracellular canaliculi and tubulovesicular system of the parietal cells. These fucose residues were added in the trans-Golgi regions to the elongating chains. The term "mucosal defense" refers to the factors that permit the gastric mucosa to withstand frequent exposure to substances with a wide range of properties including, e.g., pH, osmolarity, and detergent properties. The first level of defense consists of the factors secreted into the lumen, including acid, bicarbonate, mucus, immunoglobulins, and other antibacterial substances (Wallace and Granger 1996). Mucous secretions form a barrier that traps bicarbonate secreted by the epithelium and stops acid retrodiffusion from the gastric lumen. Moreover, bacteria become trapped in the mucus and are eventually excreted in the feces (Wallace and Granger 1996).

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“…Because this is not the subject of this work, the method will not be described. The sections were stained according to Madrid et al (1994Madrid et al ( ,1998b.…”

Section: Immunocytochemical Labelingmentioning

confidence: 99%

Lectin-Gold Localization of Fucose Residues in Human Gastric Mucosa

Madrid

Leis

Díaz‐Flores

et al. 1998

J Histochem Cytochem.

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“…In other experiments, sialic acid was removed by pretreating the sections for 2-3 h at 82°C with 0.1 M HCl [31].…”

Section: Acid Hydrolysismentioning

confidence: 99%

Lectin Binding in the Human Umbilical Cord from Normally Grown Pregnancies and from Pregnancies Complicated by Intra-Uterine Growth Retardation with Absent or Reversed Diastolic Flow

Sgambati

Brizzi²,

Marini³

et al. 2003

Neonatology

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The content and the distribution of the glycoconjugate sugar residues were investigated in the human umbilical cord from normally grown pregnancies and from pregnancies complicated by intra-uterine growth retardation with absent or reversed flow in the umbilical artery. The gestational age range was 29–41 weeks. For this purpose, a battery of eight horseradish peroxidase conjugated lectins was used (ConA, WGA, PNA, SBA, DBA, LTA, UEA I, and OOA; see text). Our data showed that in both study groups ConA, WGA and PNA (after neuraminidase digestion) and LTA, UEA I, and OOA reacted with Wharton’s jelly and vessels of the umbilical cord from the first weeks of gestation considered. SBA reactivity was observed only in the amniotic epithelial cells of the jelly and in the endothelial cells of the vessels. The reactivity with the most part of the lectins decreased or was lacking in the various components of the umbilical cord between 39 and 41 weeks of gestation in the normal group and between 36 and 37 weeks of gestation in the pathological one. Moreover, the intensity of reactivity with ConA, WGA and PNA (after neuraminidase digestion) and LTA, UEA I, and OOA in the normal group and with PNA (before and after neuraminidase digestion) and LTA and OOA in the pathological one was stronger in the peripheral portion of the jelly with respect to the adventitial one. The reactivity with ConA and UEA I was stronger in the vein than in the arteries in both study groups. Some hypotheses are reported on the role played by the sugar residues in the normal and pathological umbilical cords.

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“…In other experiments sialic acid was removed by pretreating the sections for 2-3 h at 82 8C with 0.1 M HCl [17].…”

Section: Acid Hydrolysismentioning

confidence: 99%

Lectin binding in the umbilical cord in altered glycemia

Sgambati

Marini

Thyrion

et al. 2007

European Journal of Obstetrics & Gynecology and Reproductiv

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Objective: Content and distribution of the oligosaccharides in the umbilical cord from pregnancies with altered glycemia were investigated. Study design: A prospective cohort study was conducted in the Florence Policlinic of Careggi, Italy. Samples of cord from physiological pregnancies (n = 20), from pregnancies with minor degree of glucose intolerance (n = 20) and from pregnancies with gestational diabetes mellitus (GDM) treated with insulin (n = 20) were collected. Eleven lectins were used (ConA, WGA, PNA, SBA, DBA, LTA, UEA I, OOA, GSL II, MAL II and SNA) in combination with chemical and enzymatic treatments. Results: Increase of N-acetyl-D-glucosamine and a loss of sialic acid in the umbilical cord of the cases with minor degree of glucose intolerance with respect to the other study groups was observed. D-Galactose(b1 ! 3)-N-acetyl-D-galactosamine, N-acetyl-D-galactosamine and L-fucose were in less amount in both the pathological groups with respect to the control one. Conclusion: The increase of some glycoconjugates carbohydrates and the loss of others in the umbilical cord from pregnancies with minor degree of glucose intolerance might be related to its morphofunctional alterations in a not diabetic altered glycemia. Moreover, the treatment with insulin in the GDM might play a role in restoring partially the normal glycosilation in the cord components in the attempt to renew some their functions. #

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Subcellular characterization of glycoproteins in the principal cells of human gallbladder

Cited by 30 publications

References 57 publications

Lectin-Gold Localization of Fucose Residues in Human Gastric Mucosa

Lectin-Gold Localization of Fucose Residues in Human Gastric Mucosa

Lectin Binding in the Human Umbilical Cord from Normally Grown Pregnancies and from Pregnancies Complicated by Intra-Uterine Growth Retardation with Absent or Reversed Diastolic Flow

Lectin binding in the umbilical cord in altered glycemia

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