Subantimicrobial dose doxycycline as adjunctive treatment for periodontitis

Preshaw, Philip M.; Hefti, Arthur F.; Jepsen, Søren; Etienne, Daniel; Walker, Clay; Bradshaw, Mark

doi:10.1111/j.1600-051x.2004.00558.x

Cited by 134 publications

(115 citation statements)

References 58 publications

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“…Antibiotic therapy is now being used in addition to scaling and curettage (mechanical treatment), when mechanical treatment alone is not effective (7,8). The identification and quantification of periodontopathic bacteria are useful for confirming the effects of eliminating periodontopathic bacteria by antibiotic therapy (9).…”

Section: Introductionmentioning

confidence: 99%

Quantification of Periodontopathic Bacteria in Saliva Using the Invader Assay

et al. 2012

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SUMMARY: When quantifying periodontopathic bacteria, it is important to use a convenient method that does not produce false negative results. The Invader assay is a convenient method because it does not involve gene amplification. The purpose of this study was to evaluate the validity of the Invader assay to quantify periodontopathic bacteria. The Invader technology was applied in quantifying five periodontopathic bacteria (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, and Treponema denticola). The Invader assay produced a linear quantitative detection range over concentrations spanning seven exponential values, with a detection limit of 10 3.7 copies/tube and intra-day and inter-day variance of 0.1z to 4.7z and 0.1z to 3.4z, respectively, in quantifying five periodontopathic bacteria. We compared the results of the Invader assay with those of real-time polymerase chain reaction (PCR) performed for quantifying five periodontopathic bacteria in 22 patients with periodontitis. Among the Invader-detectable bacterial strains of each species, significant correlations were observed in the counts of concerned bacterial species between these two methods, with correlation coefficients ranging from 0.757 to 0.996. This study validated repeatability and reproducibility of the Invader assay in quantifying periodontopathic bacteria and demonstrated consistent agreement between the Invader assay and real-time PCR in quantifying periodontopathic bacteria.

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Section: Introductionmentioning

confidence: 99%

Quantification of Periodontopathic Bacteria in Saliva Using the Invader Assay

et al. 2012

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“…Recently, in the field of dentistry, low-dose doxycycline hyclate (20 mg) has been used to treat and prevent periodontitis by inhibiting collagenase. 3 The authors wanted to investigate the effectiveness of such therapy in the chronic meibomian gland dysfunction patients.One hundred fifty patients were selected (55 male, 95 female) that reported to the department of ophthalmology between January and December of 2003 and newly diagnosed with chronic meibomian gland dysfunction with grade 2 or worse meibomian gland destruction or meibomian gland …”

mentioning

confidence: 99%

mentioning

confidence: 99%

The Effect of Low-Dose Doxycycline Therapy in Chronic Meibomian Gland Dysfunction

Yoo

Lee

Chang

2005

Korean J Ophthalmol

103

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Blepharitis is a relatively common disorder that manifests various clinical signs such as foreign body sensation, tearing and dry sensation, etc. The complete treatment of this condition is believed to be difficult because of abnormal meibomian gland function and changes in the normal flora of conjunctiva. Consequently, the course is often chronic, frustrating both clinicians and patients. Meibomian gland dysfunction is a common type of chronic blepharitis and although it is so common it can be easy to overlook clinically. Because symptoms can improve with appropriate treatment, it is very important to identify the disorder in patients who complain of dry eye symptoms.Chronic meibomian gland dysfunction particularly affects the lipid layer of tear film, destabilizing the tear film and quickening the tear film's evaporation, and ultimately causes many disorders of the external eye, including dry eye syndrome. 1In order to treat this dysfunction, physicians have reported prescribing 200 mg twice-daily doxycycline (α -6 deoxy-5 hydroxytetracycline), a long acting analogue of tetracycline that inhibits collagenase and also has antichemotactic effects, thus improving patients' symptoms by stabilizing the lipid layer of tear film. Recently, in the field of dentistry, low-dose doxycycline hyclate (20 mg) has been used to treat and prevent periodontitis by inhibiting collagenase. 3 The authors wanted to investigate the effectiveness of such therapy in the chronic meibomian gland dysfunction patients.One hundred fifty patients were selected (55 male, 95 female) that reported to the department of ophthalmology between January and December of 2003 and newly diagnosed with chronic meibomian gland dysfunction with grade 2 or worse meibomian gland destruction or meibomian gland Department of Ophthalmology, Dankook University College of Medicine, Cheonan, KoreaPurpose: The aim was to investigate the effect of low dose doxycycline (20 mg) therapy in patients with chronic meibomian gland dysfunction that were refractory to conventional therapy. Methods: The randomized prospective study enrolled 150 patients (300 eyes) who have chronic meibomian gland dysfunction and who didn't respond to lid hygiene and topical therapy for more than 2 months. All topical therapy was stopped for at least 2 weeks prior to beginning the study. After conducting the tear break up time test (TBUT) and Schirmer test, the authors randomly divided the patients into three groups a high dose group (doxycycline, 200 mg, twice a day), a low dose group (doxycycline, 20 mg, twice a day) and a control group (placebo). After one month, the author repeated the TBUT and Schirmer tests, and analyzed the degree of symptomatic improvement. Results: Compared to the control group, both the high and low dose group showed statistically significant differences after treatment in TBUT, Schirmer test, the number of symptoms reported and the degree of improvement of subjective symptoms. However, there was no statistically significant difference between the high and low dose ...

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“…Minocycline and doxycycline are synthetic tetracyclines, both these drugs have the characteristics to inhibit collagenase enzymes [21].…”

Section: Matrix Metalloproteinase Inhibitorsmentioning

confidence: 99%