Subacute toxicity study of the ethanolic extract of <i>Mesua ferrea</i> (L.) flowers in rats

Shirsat-John, Pallavi; Ziyaurrahman, A. R.; Kashikar, Rama; Athavale, Manjushree; Athavale, Tejas; Taware, Pritam; Saldanha, Tina; Kolhe, Swati; Tembhurne, S. V.

doi:10.1080/01480545.2020.1847134

Cited by 8 publications

(8 citation statements)

References 27 publications

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“…Subacute toxicity of the ethanolic extract of Mesua ferrea L. flowers in rats showed that at the high dose, it did not have any serious toxicity [74]. In addition, we studied the effect of the MFE extract on memory deficit induced by scopolamine in ICR mice.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Ethanol Extract from Mesua ferrea Linn Flower on Alzheimer’s Disease and Its Underlying Mechanism

Plekratoke

Boonyarat

Monthakantirat

et al. 2023

CIMB

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The effects of Mesua ferrea Linn flower (MFE) extract on the pathogenic cascade of Alzheimer’s disease (AD) were determined by an in vitro and cell culture model in the search for a potential candidate for the treatment of AD. The 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay exhibited that the MFE extract had antioxidant activities. According to the Ellman and the thioflavin T method’s result, the extracts could inhibit acetylcholinesterase and β-amyloid (Aβ) aggregation. Studies on neuroprotection in cell culture found that the MFE extract could reduce the death of human neuroblastoma cells (SH-SY5Y) caused by H2O2 and Aβ. Western blot analysis exhibited that the MFE extract alleviated H2O2-induced neuronal cell damage by downregulating the pro-apoptotic proteins, including cleaved caspase-3, Bax, and by enhancing the expression of anti-apoptotic markers including MCl1, BClxl, and survivin. Moreover, MFE extract inhibited the expression of APP, presenilin 1, and BACE, and increased the expression of neprilysin. In addition, the MFE extract could enhance scopolamine-induced memory deficit in mice. Overall, results showed that the MFE extract had several modes of action related to the AD pathogenesis cascade, including antioxidants, anti-acetylcholinesterase, anti-Aβ aggregation, and neuroprotection against oxidative stress and Aβ. Therefore, the M. ferrea L. flower might be a possibility for further development as a medication for AD.

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Section: Discussionmentioning

confidence: 99%

The Effect of Ethanol Extract from Mesua ferrea Linn Flower on Alzheimer’s Disease and Its Underlying Mechanism

Plekratoke

Boonyarat

Monthakantirat

et al. 2023

CIMB

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“…It is traditionally used as a diuretic, antipyretic, expectorant, cardiotonic, carminative, and antibacterial treatment for colds and asthma, as well as for its antiemetic, anthelminthic, aphrodisiac, and antidote properties. Antioxidant, estrogenic and progestational activity, analgesic activity, hepatoprotective, antimicrobial, anticancer, antiulcer, antivenom, antispasmodic, anti-inflammatory, antiasthmatic, immunomodulatory, tyrosinase and elastase inhibitory activity, and antioxidant are the reported validated effects of this medicinal plant [4,5]. Phytochemical analysis of the plant reveals the presence of flavanone glycosides, xanthones, coumarins, and flavones [6].…”

Section: Introductionmentioning

confidence: 99%

Quantification of β-Sitosterol by Validated High Performance Thin Layer Chromatography (HPTLC) Densitometric Method in the Flowering Buds of <i>Mesua ferrea</i> Linn

Sutradhar,

R. Sarkar

2023

J. Sci. Res.

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The medium-sized to large-sized tree Mesua ferrea Linn, commonly known as Nagakesara in India, can grow to be around 20 to 30 meters high and has incredibly durable wood. The fruiting season runs from January to March and April to October. The buds are globular, 2mm diagonally, and have scales in the axillary buds. It includes a significant amount of β-sitosterol amides, which are important from a biological perspective. In the current study, we used high-performance thin-layer chromatography (HPTLC) in association with densitometry to quantify these marker chemicals from Mesua ferrea Linn flowering buds. The technique was found to be accurate. For intra-day analysis, the relative standard deviation (RSD) values of β-sitosterol ranged from 1.16 to 1.60%, while for inter-day analyses, the values ranged from 0.68 to 1.77%. By performing a recovery study at three different levels for the standard chemicals, the accuracy of the approach was assessed, and average recoveries were determined to be 99.98%. It was observed that this TLC-densitometric approach was accurate, specific, particular, sensitive, and easy. For the simultaneous analysis of β-sitosterol from flowering buds of Mesua ferrea Linn, it can be employed in regular quality control.

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“…The buds are globular 2 mm diagonally, and scales are in axillary buds. This herbal plant's flowering and fruiting times are January to March and April to October [5,6].…”

Section: Introductionmentioning

confidence: 99%

Qualitative assessment of the flowering buds of Mesua ferrea Linn with special emphasize on HPTLC and universal DNA bar-coding technique and evaluation of its antimicrobial potential

Sutradhar,

Sarkar

2024

Futur J Pharm Sci

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Background The study has been scientifically exploring the powder sample and extracts of flowering buds of Mesua ferrea Linn (FBMF) based on pharmacognostic and phytochemical parameters. The medicinal plant Mesua ferrea Linn was identified by Botanical verification and the universal DNA bar-coding technique. The FBMF powder’s quality was performed by the micromeritics properties like bulk density, tapped density, angle of repose, Hausner ratio, Carr’s index, and optical microscopy method, and physicochemical evaluations were performed by the swelling index, foaming index, loss on drying, extractive values, and ash values. The extract’s preliminary phytochemical screening was accomplished by the alkaloids, flavonoids, tannins, steroids, carbohydrates, and glycoside tests. To ensure the presence of a bioactive compound of each FBMF extract by qualitative HPTLC study against the reference β-sitosterol Rf value of 0.83 was revealed at 254 nm with a developed solvent system toluene, ethyl acetate, and acetic acid (6:2:0.1) v/v. Many pharmacological benefits, including those related to wound healing, nonalcoholic fatty liver disease, analgesic, sedative effects, immunomodulatory, anticancer, antimicrobial, anti-inflammatory, anxiolytic, and atopic dermatitis, have been demonstrated by this phytosterol. An antimicrobial study was carried out by a well diffusion method and, lastly, measured minimum inhibitory concentration and compared to the marketed active pharmaceutical component. Numerous skin infections of more invasive, serious illnesses are caused by potential antimicrobial activity. Results The universal DNA bar-coding of plant Mesua ferrea Linn has shown a high percentage of identity. The micrometrics properties and physicochemical evaluation of the powder sample of FBMF were in an acceptable range. The preliminary phytochemical screening shows that all extracts contain steroids and carbohydrates. Moreover, flavonoids were found in ethyl acetate and ethanol, and a qualitative HPTLC study confirmed that every extract contains β-sitosterol. N-hexane extract of FBMF shows the potential antimicrobial activity with Staphylococcus aureus, and the MIC value was observed at 0.062 mg/ml. Conclusion Our research demonstrated that the plant Mesua ferrea Linn has been successfully authenticated by a DNA bar-coding technique, and all extracts of FBMF contain β-sitosterol. The n-hexane solvent extracts have shown the potential highest antimicrobial effects compared to other extracts. These results support using n-hexane extracts as a traditional medicine for treating several diseases. Graphical Abstract

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Subacute toxicity study of the ethanolic extract of Mesua ferrea (L.) flowers in rats

Cited by 8 publications

References 27 publications

The Effect of Ethanol Extract from Mesua ferrea Linn Flower on Alzheimer’s Disease and Its Underlying Mechanism

The Effect of Ethanol Extract from Mesua ferrea Linn Flower on Alzheimer’s Disease and Its Underlying Mechanism

Quantification of β-Sitosterol by Validated High Performance Thin Layer Chromatography (HPTLC) Densitometric Method in the Flowering Buds of <i>Mesua ferrea</i> Linn

Qualitative assessment of the flowering buds of Mesua ferrea Linn with special emphasize on HPTLC and universal DNA bar-coding technique and evaluation of its antimicrobial potential

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