Subacute Toxicity Profile of Lacidipine Nanoformulation in Wistar Rats

Shirodkar, Rupesh Kalidas; Misra, Chandrasekhar; Gh, Chethan; Shetty, Pallavi K.; Attari, Zenab; Mutalik, Srinivas; Lewis, Shaila

doi:10.1155/2015/947623

Cited by 14 publications

(5 citation statements)

References 24 publications

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“…Both sub-acute and chronic toxicity studies indicated normal serum creatine and urea values, a share painter of non-nephrotoxicity. This is in agreement with the study by Shirodkar (2015). The kidney gross pathology and histological organization appeared normal generally.…”

Section: Discussionsupporting

confidence: 93%

Sub-acute and chronic toxicity of silver nanoparticles synthesized by Azadirachta indica extract

Nghilokwa¹,

Sokei²,

Mwitari³

et al. 2020

Afr. J. Biotechnol.

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In this study, biosynthesis of silver nanoparticles (AgNPs) and its toxicity were investigated. Different functional groups responsible for adsorption, morphology and absorption of the nanoparticle were characterized using UV-Vis, Fourier transmission infrared spectroscopy (FTIR), and scanning electron microscope (SEM) analyses, respectively. The toxicity of orally administered Azadirachta indica AgNPs was assessed in Swiss albino rats. Sub-acute toxicity was determined in daily dosages from 30-0.3 mg/kg body weight for 28 days. Chronic toxicity was determined in two dosages 30 and 10 mg/kg body weight for 180 days. Control groups were included and were administered with distilled water. The UV-Vis spectroscopy showed surface plasmon resonance of 430 nm for the silver nanoparticle. The FTIR spectrum showed primary-N-H bond and secondary-C-N amides. The nanoparticles synthesized were 45 nm average sizes. There were no significant differences (P>0.05) observed between the packed cell volume (PCV), animals body weight of the control and treatment groups in sub-acute and chronic toxicity. Portal hepatitis in the liver at the 30 mg/kg b. wt was noted. Hence, histopathology examinations confirmed the liver damage noted in clinical biochemistry. Kidneys histological organization appeared normal generally with glomeruli and tubules visible. Our results demonstrate that A. indica silver nanoformulation may be safe at daily dosage of up-to 10 mg/kg b. wt. However, it indicates that the A. indica silver nanoformulation on daily use at 30 mg/kg may lead to liver damage.

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Section: Discussionsupporting

confidence: 93%

Sub-acute and chronic toxicity of silver nanoparticles synthesized by Azadirachta indica extract

Nghilokwa¹,

Sokei²,

Mwitari³

et al. 2020

Afr. J. Biotechnol.

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“…Additional AST enzyme will be released into the bloodstream when liver is damaged that indicates a hepatic injury in the host ( Sharma & Shukla, 2011 ). ALT level is a main indicator in the detection of liver damage by drug or hepatotoxins in the bodies ( Shirodkar et al, 2015 ). Eventually, the increase is AST level caused an elevation in the level of ALT ( Giannini, Testa & Savarino, 2005 ).…”

Section: Resultsmentioning

confidence: 99%

Characterization and toxicity of citral incorporated with nanostructured lipid carrier

Nordin

Yeap

Zamberi

et al. 2018

PeerJ

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The nanoparticle as a cancer drug delivery vehicle is rapidly under investigation due to its promising applicability as a novel drug delivery system for anticancer agents. This study describes the development, characterization and toxicity studies of a nanostructured lipid carrier (NLC) system for citral. Citral was loaded into the NLC using high pressure homogenization methods. The characterizations of NLC-citral were then determined through various methods. Based on Transmission Electron Microscope (TEM) analysis, NLC-Citral showed a spherical shape with an average diameter size of 54.12 ± 0.30 nm and a polydipersity index of 0.224 ± 0.005. The zeta potential of NLC-Citral was −12.73 ± 0.34 mV with an entrapment efficiency of 98.9 ± 0.124%, and drug loading of 9.84 ± 0.041%. Safety profile of the formulation was examined via in vitro and in vivo routes to study its effects toward normal cells. NLC-Citral exhibited no toxic effects towards the proliferation of mice splenocytes. Moreover, no mortality and toxic signs were observed in the treated groups after 28 days of treatment. There were also no significant alterations in serum biochemical analysis for all treatments. Increase in immunomodulatory effects of treated NLC-Citral and Citral groups was verified from the increase in CD4/CD3 and CD8/CD3 T cell population in both NLC-citral and citral treated splenocytes. This study suggests that NLC is a promising drug delivery system for citral as it has the potential in sustaining drug release without inducing any toxicity.

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“…Based on the results of this study, there was slight to no significant changes or toxicity damage occurred in all the treated mice. The detection of liver damage by hepatoxins or drug in the body can be indicated by the level of ALT [ 50 ]. When liver damage happen, additional AST level will be released to the blood as an indicator of a hepatic injury in the host [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Physicochemical characterization, cytotoxic effect and toxicity evaluation of nanostructured lipid carrier loaded with eucalyptol

Izham

Hussin

Rahim

et al. 2021

BMC Complement Med Ther

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Background Eucalyptol is an active compound of eucalyptus essential oil and was reported to have many medical attributes including cytotoxic effect on breast cancer cells. However, it has low solubility in aqueous solutions which limits its bioavailability and cytotoxic efficiency. In this study, nanostructured lipid carrier loaded with eucalyptol (NLC-Eu) was formulated and characterized and the cytotoxic effect of NLC-Eu towards breast cancer cell lines was determined. In addition, its toxicity in animal model, BALB/c mice was also incorporated into this study to validate the safety of NLC-Eu. Methods Eucalyptol, a monoterpene oxide active, was used to formulate the NLC-Eu by using high pressure homogenization technique. The physicochemical characterization of NLC-Eu was performed to assess its morphology, particle size, polydispersity index, and zeta potential. The in vitro cytotoxic effects of this encapsulated eucalyptol on human (MDA MB-231) and murine (4 T1) breast cancer cell lines were determined using the MTT assay. Additionally, acridine orange/propidium iodide assay was conducted on the NLC-Eu treated MDA MB-231 cells. The in vivo sub-chronic toxicity of the prepared NLC-Eu was investigated using an in vivo BALB/c mice model. Results As a result, the light, translucent, milky-colored NLC-Eu showed particle size of 71.800 ± 2.144 nm, poly-dispersity index of 0.258 ± 0.003, and zeta potential of − 2.927 ± 0.163 mV. Furthermore, the TEM results of NLC-Eu displayed irregular round to spherical morphology with narrow size distribution and relatively uniformed particles. The drug loading capacity and entrapment efficiency of NLC-Eu were 4.99 and 90.93%, respectively. Furthermore, NLC-Eu exhibited cytotoxic effects on both, human and mice, breast cancer cells with IC50 values of 10.00 ± 4.81 μg/mL and 17.70 ± 0.57 μg/mL, respectively at 72 h. NLC-Eu also induced apoptosis on the MDA MB-231 cells. In the sub-chronic toxicity study, all of the studied mice did not show any signs of toxicity, abnormality or mortality. Besides that, no significant changes were observed in the body weight, internal organ index, hepatic and renal histopathology, serum biochemistry, nitric oxide and malondialdehyde contents. Conclusions This study suggests that the well-characterized NLC-Eu offers a safe and promising carrier system which has cytotoxic effect on breast cancer cell lines.

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Subacute Toxicity Profile of Lacidipine Nanoformulation in Wistar Rats

Cited by 14 publications

References 24 publications

Sub-acute and chronic toxicity of silver nanoparticles synthesized by Azadirachta indica extract

Sub-acute and chronic toxicity of silver nanoparticles synthesized by Azadirachta indica extract

Characterization and toxicity of citral incorporated with nanostructured lipid carrier

Physicochemical characterization, cytotoxic effect and toxicity evaluation of nanostructured lipid carrier loaded with eucalyptol

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