Subacute effects of 2,4-dichlorophenoxyacetic herbicide on antioxidant defense system and lipid peroxidation in rat erythrocytes

Tayeb, Wafa; Nakbi, Amel; Chargui, Issam; Cheraief, Imed; Miled, A.; Hammami, Mohamed

doi:10.1016/j.pestbp.2011.01.004

Cited by 38 publications

(16 citation statements)

References 75 publications

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“…In fact, red blood cells membranes are highly susceptible to lipid peroxidation because they are rich in polyunsaturated fatty acids. In addition, erythrocytes contain haeme–iron, an essential requirement in hydrogen peroxide‐stimulated oxidation of the red cell membrane lipids . Considering the importance of artesunate–amodiaquine combination in malaria therapy and the fact that artesunate targets the blood stage of the malaria parasite, the present study was undertaken to investigate the toxic potential of artesunate as a monotherapy and in combination with amodiaquine on the plasma biochemical, and erythrocyte lipid peroxidation biomarkers in a female mammalian rat model.…”

Section: Introductionmentioning

confidence: 99%

Artesunate–amodiaquine combination therapy in the absence of malarial parasite infection induces oxidative damage in female rats

Abolaji

Osedeme

Olusemire

2013

Cell Biochemistry & Function

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Artesunate-amodiaquine is among the most widely available artemisinin combination therapy used as treatment regimen for uncomplicated Plasmodium falciparum malaria. Our aim was to evaluate clinical routine markers of liver and renal functions, lipid profile levels and lipid peroxidation status in a female mammalian rat model. This was an attempt to simulate a scenario where the drugs are taken without malarial parasite infection, which is a common practice in settings where drug misuse is a common practice. Twenty female Wistar rats were randomly divided into four study groups of five animals each. Group 1 (control) received distilled water, group 2 was exposed to artesunate [2 mg/kg body weight (b.w.)], group 3 was administered with amodiaquine (6.12 mg/kg b.w.) and group 4 was co-administered with artesunate (2 mg/kg b.w.) and amodiaquine (6.12 mg/kg b.w.) for 3 days. At the end of the treatment period, animals were fasted overnight and sacrificed. Markers of liver and renal functions and lipid profile indices were evaluated in the plasma, whereas lipid peroxidation status, GSH concentration and G6PD activity were assessed in the erythrocytes. The results showed that the co-administration of artesunate and amodiaquine altered liver function markers and lipid profile indices. The drugs also induced lipid peroxidation as evidenced by the elevated level of oxidative stress marker malondialdehyde (p < 0.05). We recommend therefore that the drugs should be taken with prescription only with clinical evidence of malarial parasite infection.

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Section: Introductionmentioning

confidence: 99%

Artesunate–amodiaquine combination therapy in the absence of malarial parasite infection induces oxidative damage in female rats

Abolaji

Osedeme

Olusemire

2013

Cell Biochemistry & Function

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“…The significant decrease in the in the glucose content and increase in percent inhibition observed in the present study may be due to glucose starvation and oxidative stress (Salakhetdinova et al, 2000), disturbance in cellular metabolism (Stadtman, 1992), modification of carbohydrate (Halliwell and Gutteridge, 1989), unstable free radical species that can attack cellular components, inducing damage to lipids, proteins, DNA and carbohydrates (Mori et al, 2007). Studies have been shown that methomyl carbamate exposure promotes the oxidation damage of liver cells by enhancing peroxidation of membrane lipids (Wafa et al, 2011), which was evident by enhanced LDH activity in liver accompanied by decreased content of GSH as well as suppression of the activities of antioxidant enzymes (El-Khawaga, 2012).…”

Section: Discussionmentioning

confidence: 49%

Effect of carbaryl on biochemical contents in Escherichia coli and soil isolate Pseudomonas aeruginosa

Santhala¹

2012

J. App. Pharm. Sci.

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The wide use of carbamate pesticides in agriculture has persuaded the rapid evolution and dissemination of specific degradative pathways for the compound in soil bacteria. Carbamate insecticide, carbaryl (1-naphthyl-Nmethylcarbamate), is highly toxic with a wide range of activity and known to be metabolized by microorganisms belonging to a variety of bacterial genera by interacting with different components, thereby impairing the physiological and metabolic activities of the cell. To evaluate its toxicity, the present study was undertaken by using increasing concentrations of carbaryl (10-8 to 10-2 M) and evaluated for its toxicity to Escherichia coli with emphasis on biochemical contents such as DNA, RNA, protein, glucose utilization, growth and was further compared to the soil isolate-Pseudomonas aeruginosa at a given periods of 24-72 hrs respectively. The results indicated that carbaryl treated groups exhibited a significant (P ≤ 0.05) decrease in the levels of biochemical contents and increase in the % inhibition in these parameters was observed with an increase in dose and durational exposure to carbaryl when compared to the controls. While, in assessment with its free corresponding, the activity was less in immobilized Escherichia coli cells enlightening that immobilized system is less responsive to carbaryl. Present study suggested that carbaryl is a toxicant affecting the synthesis of biochemical contents and growth in a dose and dependent manner and alterations in the parameters of Escherichia coli and Pseudomonas aeruginosa on exposure to carbaryl are can be due to carbaryl intoxication.

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“…Whereas SOD catalyzes the conversion of superoxide radicals to hydrogen peroxide, CAT converts hydrogen peroxide into water. Therefore, SOD-CAT system provides the first defense system against oxidative stress and these enzymes work together to eliminate active oxygen species (Wafa et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Effect of Ginkgo Biloba Leaves Extract on Some Formaldehyde Induced Toxicity in hippocampus of Adult Male Albino Rats

Elkhalek

Ibrahim

2014

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

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Formaldehyde (FA) heavily impacts the everyday consumer products. It is widely used in the construction, textile, furniture, medical, chemical, and pharmaceutical industries. Exposure to FA poses a significant threat to public health. It can cause severe central nervous system impairment. Extract from the leaves of Ginkgo biloba exerts a novel spectrum of biological, pharmacological and therapeutic properties against oxidative stress, so it was of special concern to investigate the role of Ginkgo biloba leaves extract (GBE) on the neurotoxic effects of FA on hippocampus of adult male albino rats. Thirty adult male albino rats were used in the present study. They were divided into 5 equal groups: Group I negative control, Group II received distilled water by intraperitoneal (i.p.) injection. Group III received GBE (300 mg/kg/day) orally. Group IV received FA (0.2 mg/kg/day i.p. and Group V received 0.2 mg/kg FA + 300 mg/kg GBE. After the end of the study (4weeks), all rats were sacrificed and brain were dissected out and hippocampus subjected to estimation of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) catalase (CAT) and malondialdehyde (MDA) levels. Histological examination of the hippocampus and immunohistochemical staining for Glial fibrillary acidic protein (GFAP) were also done. The results of this study revealed that, the levels of SOD, CAT and GSH-Px were significantly decreased, while MDA level in hippocampus tissue were significantly increased in rats treated with FA compared to those of the controls. Microscopic examination of neurons of the hippocampus in this group revealed picnotic nuclei, vacuolar degeneration and neuronal loss. Immunostaining showed areas with significantly increased GFAP immunopositivity. Concomitant administration of GBE with FA resulted in a significant increase in antioxidant enzymes activity (SOD, GSH-Px and CAT), decreased MDA, and improvement of histopathological changes induced by FA. Also a significant reduction in expression of GFAP was observed. It was concluded that short term administration of FA induced neurotoxicity on the hippocampus of adult male albino rats, with oxidant stress and lipid peroxidation which may be a molecular mechanism involved in FA induced neurotoxicity. Furthermore, these effects were decreased by the concomitant use of GBE. It is recommended to use GBE for amelioration of toxic manifestations of formaldehyde in exposed population.

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Subacute effects of 2,4-dichlorophenoxyacetic herbicide on antioxidant defense system and lipid peroxidation in rat erythrocytes

Cited by 38 publications

References 75 publications

Artesunate–amodiaquine combination therapy in the absence of malarial parasite infection induces oxidative damage in female rats

Artesunate–amodiaquine combination therapy in the absence of malarial parasite infection induces oxidative damage in female rats

Effect of carbaryl on biochemical contents in Escherichia coli and soil isolate Pseudomonas aeruginosa

Effect of Ginkgo Biloba Leaves Extract on Some Formaldehyde Induced Toxicity in hippocampus of Adult Male Albino Rats

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