Sub-Tenon Sustained Controllable Delivery of Dexamethasone for Treating Severe Acute Experimental Uveitis

Zhao, Libei; Huang, Xian-Ju; Peng, Manqiang; Tan, Qian; Lin, Wenxiang; Khan, Mohammad Ayub; Tang, Qiongyan; Lin, Ding

doi:10.1080/09273948.2019.1643027

Cited by 1 publication

(4 citation statements)

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“…It has been shown that severe ocular inflammation could be observed in experimental uveitis models. 25 In the current study, we started the treatment 24 hours after the model induction when severe intraocular inflammation occurred. The clinical inflammatory scores of the anterior chamber and vitreous humor in the control group were gradually increased over time, whereas those in the SI group and SMS group were decreased.…”

Section: Discussionmentioning

confidence: 99%

“…In the SMS group, after anesthesia, rabbits were administered SMS in the left eyes (Figure 1), which was prepared according to our previous study. 25 Briefly, a catheter with a micro-needle (BD Intima Ⅱ Closed IV Catheter System, Becton, Dickinson Co., USA) was inserted into sub-Tenon space, the micro-needle was then removed and a catheter was placed between the Tenon's capsule and sclera, approximately 5 mm past the limbus in the superior temporal quadrant of the left eye. After that, 0.3 mL of 20 mg/mL CP (0.2 g, Jiangsu Shengdi Pharmaceutical Co., Ltd., Jiangsu, China) was released into the sub-Tenon as the initial dosage.…”

Section: In Vivo Studymentioning

confidence: 99%

“…Rabbits were intravitreally injected with Mycobacterium tuberculosis H37Ra antigen (Becton, Dickinson Co., USA) suspended in phosphate-buffered saline (PBS; 80 µg; 1 µg/µL) after pre-immunization to establish the severe experimental uveitis. [25][26][27] Rabbits were then randomly assigned to three groups: (1) SMS group (n=8): rabbits were treated with SMS of 20 mg/mL CP for 7 days (10 h/day); (2) SI group (n=8): rabbits received SI of 20 mg/mL CP for 7 days; (3) control group (n=8): rabbits did not receive any treatment.…”

Section: Experimental Uveitis Rabbit Model and Treatmentmentioning

confidence: 99%

“…Our previous work showed that sub-Tenon sustained delivery of dexamethasone effectively inhibited the severity of uveitis in rabbits. 25 However, management of severe and refractory ocular inflammation recalcitrant to corticosteroids therapies often requires combined application of CP. Therefore, our current study aimed to investigate the delivery efficiency of CP via a sub-Tenon micro-perfusion system (SMS) to the vitreous and retina, and assess its therapeutic efficacy in rabbits with severe experimental uveitis.…”

Section: Introductionmentioning

confidence: 99%

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<p>Efficacy of Sub-Tenon Micro-Perfusion of Cyclophosphamide in Rabbits with Severe Ocular Inflammation</p>

Zhao

Peng

Lin

et al. 2020

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Purpose To explore the feasibility of cyclophosphamide (CP) via a sub-Tenon micro-perfusion system (SMS) in rabbits, and assess its therapeutic efficacy in severe ocular inflammation. Materials and Methods Distribution and pharmacokinetics of CP were evaluated in vivo, and the concentrations of CP in plasma, vitreous humor, and retina/choroid were quantitated by ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) at different time points. After induction of severe experimental uveitis, rabbits were divided into three groups (n=8 in each): the SMS group, subconjunctival injection (SI) group, and control group. Clinical inflammatory score was assessed in rabbits. Electroretinography and histopathology were performed on post-treatment day 8. Statistical analyses were performed using Mann–Whitney and Kruskal–Wallis tests. P -value less than 0.05 was considered significant. Results The concentrations of CP in vitreous humor and retina/choroid in the SMS group were significantly higher than that of the SI group at 3, 6, 10, and 24 hours ( P <0.01), while plasmatic CP concentrations were comparable at all time points in the SMS group and SI group ( P >0.05). The SMS group showed significantly less inflammation compared to the control group and SI group. Furthermore, the restoration of retinal structure and function were more obvious in the SMS group compared with conventional SI application. Conclusion Sub-Tenon micro-perfusion of CP exhibited satisfied therapeutic efficacy in rabbits with severe ocular inflammation and may provide a promising alternative for controlling ocular inflammatory disease and immune-mediated ocular diseases.

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Section: Discussionmentioning

confidence: 99%

Section: In Vivo Studymentioning

confidence: 99%