Sub‐minute determination of rifampicin and isoniazid in fixed dose combination tablets by capillary zone electrophoresis with ultraviolet absorption detection

Abstract

A validated sub minute capillary zone electrophoresis method with direct ultraviolet absorption detection for simultaneous determination of isoniazid and rifampicin in fixed‐dose combination tablets was developed. Background electrolyte was defined based on the analytes effective mobility curve and it was composed by 20 mmol/L of sodium carbonate/sodium bicarbonate at pH 10.2. A careful validation procedure considering the main figures of merit was performed. Regression models were satisfactory for isoniazid and rifampicin, showing no lack of fit within 95% significance interval. Interday and intraday precision were evaluated in standard and sample and slight relative standard deviations were achieved for concentration, area, and migration time. Recovery values for accuracy in two levels were 99.97 and 90.08% for isoniazid and 95.45 and 95.12% for rifampicin. The limits of detection for isoniazid and rifampicin were 0.22 and 0.34 mg/L, respectively, and the limits of quantification were 0.74 and 1.13 mg/L, respectively. Method selectivity was verified by injecting diluent, background electrolyte, a standard mixture, and a sample, confirming no interferent peaks. The method proved to be simple, environmentally friendly, sensitive, and was successfully applied for simultaneous quantification of isoniazid and rifampicin in fixed‐dose combination tablets.

Enhanced Photoelectrochemical Detection of an Analyte Triggered by Its Concentration by a Singlet Oxygen-Generating Fluoro Photosensitizer

“…Importantly, the reported analysis method is more sensitive than the spectrophotometric assay (LOD = 0.7 μM) as well as chromatographic , or electrophoretic methods coupled with a UV-detector (Table S1). Beside the high sensitivity, our photoelectrochemical method is also comparatively simple to set up and use.…”

Capillary electrophoretic methods for quality control analyses of pharmaceuticals: A review

“…A list of CZE‐UV methods applicable to quality control analyses of pharmaceuticals published during the last 5 years is provided in Table 1 [30–52]. Such a typical procedure was introduced by Mufusama et al [33].…”

“…A concept of effective mobility curves in the function of pH was used for BGE optimization of a method enabling separation and quantification of isoniazid and rifampicin in fixed‐dose combination tablets [44]. As a result, separation of the analytes in less than 1 min, and comparable sensitivity with previously published procedures based on (U)HPLC was achieved.…”

Rapid method for simultaneous determination of ascorbic acid and zinc in effervescent tablets by capillary zone electrophoresis with contactless conductivity detection

“…CE is a powerful separation technique that has several desirable advantages, such as low cost, minimal reagent and sample consumption (environmentally friendly), high separation efficiency, simple instrumentation, and often provides short analysis time . CE with capacitively coupled contactless conductivity detection (C 4 D) has been widely used for the separation and detection of a large number of compounds .…”