Sub-littoral and supra-littoral amphipods respond differently to acute thermal stress

Bedulina, Daria; Zimmer, Martin; Timofeyev, Maxim A.

doi:10.1016/j.cbpb.2010.01.004

Cited by 37 publications

(26 citation statements)

References 61 publications

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“…Pejus (T p ) and critical (T c ) temperatures and the respective pejus range for several marine invertebrate and fish species; not detected HSP70 mRNA expression measured by quantitative real-time PCR in the heart, were similar between the 3 investigated species (data not shown). This is in contrast to a study comparing sub-and supra-littoral amphipods Gammarus oceanicus and Orchestia gammarellus, respectively (Bedulina et al 2010), which found elevated baseline levels of HSP70 protein in the supra-littoral species and interprets this as an evolutionary adaptation to the more stressful environment. Similarly, Tomanek (2010) indicates that subtidal animals have a wide range of temperatures over which they can synthesize HSPs, while intertidal animals that are living close to their upper thermal limits have a reduced ability to acclimate further to increased temperatures.…”

Section: Discussioncontrasting

confidence: 53%

Enhancing thermal tolerance by eliminating the pejus range: a comparative study with three decapod crustaceans

Jost¹,

Podolski²,

Frederich³

2012

Mar. Ecol. Prog. Ser.

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Marine invertebrates in the intertidal and subtidal zones are often exposed to highly variable environmental conditions, especially rapid changes in temperature. The ability to survive at different temperatures has previously been described using an extended version of Shelford's law of tolerance, with optimum, pejus (Latin: 'turning worse'), and pessimum ranges, and the respective thresholds, critical (T c ) and pejus (T p ) temperatures, that mark the transition from one range into the next. The width of the pejus range, in which the scope for activity gradually declines, varies among species. We tested the hypothesis that the width of the pejus range is correlated to the temperature stability of the species' respective habitats. We used locomotor activity, heart rate, lactate accumulation, heat shock protein 70 (HSP70) levels, and the activation of AMPactivated protein kinase (AMPK) to identify T c and T p in 3 decapod crustaceans: green crab Carcinus maenas, rock crab Cancer irroratus, and lobster Homarus americanus. We found speciesspecific patterns of temperature-induced changes in all parameters, especially in HSP70 protein and AMPK activity. The width of the pejus range (between T p and T c ) was 8 to 12°C for rock crabs and 12 to 16°C for lobsters. Most importantly, green crab, the most temperature-tolerant of our 3 species and which lives in a highly variable habitat, switched directly from optimum to pessimum range, meaning that the pejus range was eliminated completely. Additionally, even lethal temperatures did not activate AMPK in green crabs, pointing to a different cellular tolerance strategy than in rock crabs and lobsters. This modified tolerance pattern might represent a broader strategy to enhance physiological tolerance in a highly variable habitat. KEY WORDS: AMP-activated protein kinase · AMPK · Heat shock protein 70 · HSP70 · Temperature stress · Critical temperatures Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 444: 263-274, 2012 264 ranges at both extremes. T c characterizes the transition into the pessimum range. However, in its natural environment an animal is frequently exposed to suboptimal conditions, often well before T c is attained. Consequently, Frederich & Pörtner (2000) added 'pejus temperature' (T p ) and 'pejus range' (from the Latin for 'turning worse') to this concept. In the pejus range, which is between the optimum and pessimum ranges, animals can still survive, but with a reduced scope for aerobic activity. Subsequent studies have identified T p and pejus ranges in various species of marine invertebrates and fish (reviewed by Pört-ner 2010). T p and T c differ by several degrees, thus in dicating a well-defined and broad pejus range. However, the width of the pejus range appears to vary among species, and the outstanding question of whether this is associated with habitat stability needs to be addressed.In a previous study on the rock crab Cancer irroratus, we showed that T p can be identified on the cell...

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Section: Discussioncontrasting

confidence: 53%

Enhancing thermal tolerance by eliminating the pejus range: a comparative study with three decapod crustaceans

Jost¹,

Podolski²,

Frederich³

2012

Mar. Ecol. Prog. Ser.

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“…Differential expression of various HSP genes could be responding to trade-offs between maintaining specific HSPs at high basal levels for protection versus the associated energetic costs of maintaining those levels. Such trade-offs would affect the quantity of individual HSP isoforms produced in response to a thermal stress event, and could be due to locally adapted habitat preferences (Tomanek, 2010;Bedulina et al, 2010).…”

Section: Molecular Response To Acute Heat Shockmentioning

confidence: 99%

Comparative physiological, biochemical, and molecular thermal stress response profiles for two Unionid freshwater mussel species

Payton

Johnson

Jenny

2016

Journal of Experimental Biology

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Freshwater mussels, aquatic keystone species, are in global decline. Long life spans, sedentary lifestyles, and unique reproductive strategies involving obligate parasitic stages make unionid freshwater mussels particularly sensitive to environmental perturbations resulting from global climate change. A greater understanding of the mechanisms by which closely related species differ in their response to thermal challenge is critical for successful conservation and management practices. As such, both an acute heat shock and a chronic warming simulation were conducted in order to evaluate responses between hypothesized thermally tolerant (Villosa lienosa) and thermally sensitive (Villosa nebulosa) freshwater mussels in response to predicted thermal warming. Multiple biological responses were quantified, including mortality, condition index, growth rates, glycogen and triglyceride content, and candidate gene expression. During acute heat shock, both species upregulated HSP90 and HSP70, although V. lienosa showed consistently greater transcript levels during upregulation. This pattern was consistent during the chronic warming simulation, with V. nebulosa showing greater induction of HSP60. Chronic warming stimulated increases in condition index for V. nebulosa; however, declines in growth rates during a recovery period were observed with no concurrent change in tissue glycogen levels. This contrasts with V. lienosa, where tissue glycogen significantly increased during chronic warming, although no response was observed for condition index or growth rates. These biological differences might indicate disparate thermal stress response mechanisms correlated with metabolic demands and resource utilization, and could thus be a factor influencing current ranges of these two species and their ability to cope with future persistent warming in their native habitats.

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“…local mean or maximum temperature [7][8][9][10][11]. Local adaptation and phenotypic plasticity may evolve together [12 -16] and it is often difficult to disentangle their relative contributions [13,17,18]. Furthermore, phenotypic plasticity may also adapt locally.…”

Section: Introductionmentioning

confidence: 99%

Adaptive phenotypic plasticity and local adaptation for temperature tolerance in freshwater zooplankton

2014

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Many organisms have geographical distributions extending from the tropics to near polar regions or can experience up to 308C temperature variation within the lifespan of an individual. Two forms of evolutionary adaptation to such wide ranges in ambient temperatures are frequently discussed: local adaptation and phenotypic plasticity. The freshwater planktonic crustacean Daphnia magna, whose range extends from South Africa to near arctic sites, shows strong phenotypic and genotypic variation in response to temperature. In this study, we use D. magna clones from 22 populations (one clone per population) ranging from latitude 08 (Kenya) to 668 North (White Sea) to explore the contributions of phenotypic plasticity and local adaptation to high temperature tolerance. Temperature tolerance was studied as knockout time (time until immobilization, T imm ) at 378C in clones acclimatized to either 208C or 288C. Acclimatization to 288C strongly increased T imm , testifying to adaptive phenotypic plasticity. At the same time, T imm significantly correlated with average high temperature at the clones' sites of origin, suggesting local adaptation. As earlier studies have found that haemoglobin expression contributes to temperature tolerance, we also quantified haemoglobin concentration in experimental animals and found that both acclimatization temperature (AccT) and temperature at the site of origin are positively correlated with haemoglobin concentration. Furthermore, Daphnia from warmer climates upregulate haemoglobin much more strongly in response to AccT, suggesting local adaptation for plasticity in haemoglobin expression. Our results show that both local adaptation and phenotypic plasticity contribute to temperature tolerance, and elucidate a possible role of haemoglobin in mediating these effects that differs along a cold-warm gradient.

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Sub-littoral and supra-littoral amphipods respond differently to acute thermal stress

Cited by 37 publications

References 61 publications

Enhancing thermal tolerance by eliminating the pejus range: a comparative study with three decapod crustaceans

Enhancing thermal tolerance by eliminating the pejus range: a comparative study with three decapod crustaceans

Comparative physiological, biochemical, and molecular thermal stress response profiles for two Unionid freshwater mussel species

Adaptive phenotypic plasticity and local adaptation for temperature tolerance in freshwater zooplankton

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