Sub-lethal exposure to malaria vector control pesticides causes alterations in liver metabolomics and behaviour of the African clawed frog (Xenopus laevis)

Wolmarans, Nico J.; Bervoets, Lieven; Meire, Patrick; Wepener, Victor

doi:10.1016/j.cbpc.2021.109173

Cited by 4 publications

(3 citation statements)

References 64 publications

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Section: Discussionmentioning

confidence: 99%

“…Recently, metabolome technique are being used for DDT sensitive animals, such as green mussel and frog [39,47]. The in vivo study using African clawed frogs showed several pathways, including unsaturated fatty acid and galactose metabolism, which significantly changed due to DDT exposure [47]. Metabolome analyses using field-collected samples can be an important factor in determining the ecological toxicity of environmental hormones in chemically polluted areas.…”

Section: Discussionmentioning

confidence: 99%

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Investigation of dichlorodiphenyltrichloroethane (DDT) on xenobiotic enzyme disruption and metabolomic bile acid biosynthesis in DDT-sprayed areas using wild rats

et al. 2023

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Dichlorodiphenyltrichloroethane (DDT) is an organochlorine insecticide used worldwide. Several studies have reported the toxic effects of DDT and its metabolites on steroid hormone biosynthesis; 1 however, its environmental effects are not well understood. This study examined wild rats collected in DDT-sprayed areas of South Africa and quantified plasma metabolites using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). Fold change analysis of the metabolome revealed the effect of DDT on bile acid biosynthesis. Gene expression of the related enzyme in rat liver samples was also quantified. Significant association was found between DDT and gene expression levels related to constitutive androstane receptor mediated enzymes, such as Cyp2b1 in rat livers. However, our results could not fully demonstrate that enzymes related to bile acid biosynthesis were strongly affected by DDT. The correlation between DDT concentration and gene expression involved in steroid hormone synthesis in testis was also evaluated; however, no significant correlation was found. The disturbance of metabolic enzymes occurred in rat liver in the target area.Our results suggest that DDT exposure affects gene expression in wild rats living in DDT-sprayed areas. Therefore, there is a need for DDT toxicity evaluation in mammals living in DDT-sprayed areas.We could not find an effective biomarker that could reflect the mechanism of DDT exposure; however, this approach can provide new insights for future research to evaluate DDT effects in sprayed areas.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Investigation of dichlorodiphenyltrichloroethane (DDT) on xenobiotic enzyme disruption and metabolomic bile acid biosynthesis in DDT-sprayed areas using wild rats

et al. 2023

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“…Although pesticides are advantageous to use, they have potential negative effects on the environment, human health, and wildlife, especially on non-target organisms (Margni et al 2002 ). There is a paucity of research on the primary and secondary effects that these pesticides have on non-target organisms (Wolmarans et al 2022 ), including primary producers such as diatoms. Recent microcosm studies by Kock et al ( 2022 ) demonstrated that low levels of DDT, DTM and their mixtures resulted in changes in diatom community traits and decreased vitality in several diatom species.…”

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confidence: 99%

Using Confocal Microscopy and Pigment Analyses to Detect Adverse Insecticide Effects in non-target Freshwater Diatom species - a proof-of-concept Using Nitzschia palea

Kock

Pheiffer

Wepener

et al. 2023

Bull Environ Contam Toxicol

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The persistence of insecticides in aquatic environments is a cause of concern and to date hardly any studies have focused on the effects that DDT and deltamethrin have on non-target freshwater diatom communities. The application of diatoms in ecotoxicological studies is well acknowledged and therefore this study used laboratory bioassays to determine the effects that DDT and deltamethrin have on a monoculture of a diatom indicator species, Nitzschia palea. The insecticides affected the morphology of chloroplasts at all exposure concentrations. These effects were a maximum reduction in chlorophyll concentrations (4.8% and 2.3%), cell viability (51% and 42%), and increases in cell deformities (3.6% and 1.6%) following exposure to DDT and deltamethrin respectively. Based on the results we propose that methods, such as confocal microscopy, chlorophyll-α analysis and cell deformities are useful tools in assessing the effects of insecticides on diatoms.

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Environmental influences on hormones and reproduction in amphibians

Norris

2024

Hormones and Reproduction of Vertebrates, Volume 2

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Sub-lethal exposure to malaria vector control pesticides causes alterations in liver metabolomics and behaviour of the African clawed frog (Xenopus laevis)

Cited by 4 publications

References 64 publications

Investigation of dichlorodiphenyltrichloroethane (DDT) on xenobiotic enzyme disruption and metabolomic bile acid biosynthesis in DDT-sprayed areas using wild rats

Investigation of dichlorodiphenyltrichloroethane (DDT) on xenobiotic enzyme disruption and metabolomic bile acid biosynthesis in DDT-sprayed areas using wild rats

Using Confocal Microscopy and Pigment Analyses to Detect Adverse Insecticide Effects in non-target Freshwater Diatom species - a proof-of-concept Using Nitzschia palea

Environmental influences on hormones and reproduction in amphibians

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