Sub-cellular markers highlight intracellular dynamics of membrane proteins in response to abiotic treatments in rice

Abstract

BackgroundCell biology approach using membrane protein markers tagged with fluorescent proteins highlights the dynamic behaviour of plant cell membranes, not only in the standard but also in changing environmental conditions. In the past, this strategy has been extensively developed in plant models such as Arabidopsis.ResultsHere, we generated a set of transgenic lines expressing membrane protein markers to extend this approach to rice, one of the most cultivated crop in the world and an emerging plant model. Lines expressing individually eight membrane protein markers including five aquaporins (OsPIP1;1, OsPIP2;4, OsPIP2;5, OsTIP1;1, OsTIP2;2) and three endosomal trafficking proteins (OsRab5a, OsGAP1, OsSCAMP1) were obtained. Importantly, we challenged in roots the aquaporin-expressing transgenic lines upon salt and osmotic stress and uncovered a highly dynamic behaviour of cell membrane.ConclusionWe have uncovered the relocalization and dynamics of plasma membrane aquaporins upon salt and osmotic stresses in rice. Importantly, our data support a model where relocalization of OsPIPs is concomitant with their high cycling dynamics.Electronic supplementary materialThe online version of this article (10.1186/s12284-018-0209-2) contains supplementary material, which is available to authorized users.

Contribution of the arbuscular mycorrhizal symbiosis to the regulation of radial root water transport in maize plants under water deficit

“…On the other hand, aquaporin regulation may be cell-specific, being masked in wholeorgan extractions. Furthermore, many aspects of the aquaporin regulation have to be considered, as gating, cycling or internalization due to environmental stresses (Chu et al, 2018). Indeed, the activity of aquaporins must be controlled by regulation mechanisms allowing a rapid response to the frequent environmental changes that plants undergo.…”

Subcellular Localization of Rice Acyl-CoA-Binding Proteins ACBP4 and ACBP5 Supports Their Non-redundant Roles in Lipid Metabolism

“…Although rice protoplasts have been more frequently used for subcellular localization assays of rice proteins (Zhang et al, 2011;Kim et al, 2012Kim et al, , 2015Zhou et al, 2018), it is not suitable for proteins that are targeted to the extracellular space and would not be applicable for testing whether a protein is delivered to the PM or cell wall. Rice root cells, that have been successfully applied for subcellular localization assays of rice protein localization (Zhang et al, 2012;Chu et al, 2018), were successfully used in this study to overcome the limitations imposed by the use of rice protoplasts. The apoplastic localization of OsACBP5 is further supported by the predicted signal peptide of OsACBP5 by iPSORT and the secretory pathway predicted by TargetP1.1 .…”

Fluorescent reporters for functional analysis in rice leaves

“…In many plant species, imaging of fluorescent proteins is commonly reported for all major plant organs (Bellucci, Marchis, Mannucci, Bock, & Arcioni, 2005 ;Cutler, Ehrhardt, Griffitts, & Somerville, 2000 ;Mohanty et al, 2009 ). However, in rice, while they are routinely used for analysis in roots and other non-green tissues (Abiko et al, 2013 ;Chu et al, 2018 ;Kim, Yuan, Cilia, Khalfan-Jagani, & Jackson, 2002 ;Kobae & Hata, 2010 ;Wu et al, 2016 ), there are fewer examples of fluorescent reporters being used in leaves (Dangol, Singh, Chen, & Jwa, 2017 ;Johnson et al, 2005 ;Meng, Hsiao, Gao, Jiang, & Chye, 2014 ;Reynoso et al, 2018 ;Wang, Zhao, Fu, Wang, & Jiang, 2018 ;Wu et al, 2016 ). In fact, most of these reports show fluorescent protein expression in the leaf sheath (Dangol et al, 2017 ;Meng et al, 2014 ;Wang et al, 2018 ;Wu et al, 2016 ) and few show expression in the leaf blade (Johnson et al, 2005 ;Reynoso et al, 2018 ).…”