Sub-basal increases of GABA enhance the synthesis of TNF-α, TGF-β, and IL-1β in the immune system organs of the Nile tilapia

Nájera-Martínez, Minerva; López-Tapia, Brenda P.; Aguilera-Alvarado, G Paulina; Madera-Sandoval, Ruth L.; Sánchez‐Nieto, Sobeida; Girón-Pérez, Manuel Iván; Vega-López, Armando

doi:10.1016/j.jneuroim.2020.577382

Cited by 8 publications

(4 citation statements)

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“…At the late vaccination time points (21 and 70 days post injection), the vaccinated group showed significant enhancement expression of these molecules. CD80/86, the co-stimulatory molecules that expressed on the surface of maturated APCs, are widely used as biomarkers for APCs maturation. , As an important cytokine that is commonly secreted by the activated macrophages, TNF-α was known to promote the proliferation and differentiation of cytotoxic T lymphocyte (CTL), which play an important role in cellular immune responses . IgM plays a vital role in systemic immunity, and IgZ plays a specialized role in gut mucosal immunity and functionally resembles mammalian IgA. , The above results indicated that CS-G@M-M could efficiently induce the maturation of APCs and the subsequent cellular and humoral immune responses.…”

Section: Resultsmentioning

confidence: 91%

“…16,17 As an important cytokine that is commonly secreted by the activated macrophages, TNF-α was known to promote the proliferation and differentiation of cytotoxic T lymphocyte (CTL), which play an important role in cellular immune responses. 44 IgM plays a vital role in systemic immunity, and IgZ plays a specialized role in gut mucosal immunity and functionally resembles mammalian IgA. 45,46 The above results indicated that CS-G@M-M could efficiently induce the maturation of APCs and the subsequent cellular and humoral immune responses.…”

Section: Delivery Kinetics Of Npsmentioning

confidence: 89%

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Application of Biomimetic Cell-Derived Nanoparticles with Mannose Modification as a Novel Vaccine Delivery Platform against Teleost Fish Viral Disease

Zhang

Guo

et al. 2020

ACS Biomater. Sci. Eng.

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Although cell membrane-coated nanoparticles are widely used as a promising nanodelivery platform, a few studies reported their application in developing the teleost nanovaccine delivery system. Here, we present a biomimetic vaccine delivery platform by encapsulating chitosan-loaded DNA vaccine with teleost erythrocytes membrane modified by mannose. The developed CS-G@M-M nanovaccine delivery platform shows good biocompatibility in vivo and in vitro. With further modification of mannose moiety, the constructed CS-G@M-M showed enhanced uptake by antigen-presenting cells (APCs) and increased accumulation of CS-G@M-M in immune tissues including spleen, kidney, and hindgut. Critically, using a quantitative real-time polymerase chain reaction (qRT-PCR) assay, increased mRNA levels of immune-related genes were detected in spleen and hindgut of vaccinated fish. Moreover, through enzyme-linked immunosorbent assay (ELISA), we found that the levels of CD80/86, TNF-α, IgM, and IgZ in spleen and hindgut were significantly increased. To evaluate the immunoprotection efficacy of the constructed nanovaccine, spring viremia of carp virus (SVCV), a rhabdovirus of worldwide importance that requires notification within 48 h to the International Office of Epizootics once detected, was used as a model for virus challenge. We carried out three challenge tests on 3rd, 21st, and 70th days post vaccination, respectively. Notably, CS-G@M-M nanovaccine showed durability of immunoprotection efficacy that could protect zebrafish from SVCV challenge. This work presents a novel design of smart teleost erythrocytes membrane-coated nanoparticles, which are inherently biocompatible, promising for eliciting robust adaptive immune responses in preventing fish viral diseases.

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Section: Resultsmentioning

confidence: 91%

Section: Delivery Kinetics Of Npsmentioning

confidence: 89%

Application of Biomimetic Cell-Derived Nanoparticles with Mannose Modification as a Novel Vaccine Delivery Platform against Teleost Fish Viral Disease

Zhang

Guo

et al. 2020

ACS Biomater. Sci. Eng.

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“…The role of GABAergic signalling in relation to immune response has regained interest during the COVID‐19 pandemic. 41 , 42 GABA A receptors are expressed in antigen‐presenting cells (APCs) and activation of these receptors can hyperpolarize APCs, thereby reducing their activity. 43 This may lead to a reduction in the body's inflammatory response.…”

Section: Gaba a Receptors Stimulation Prevent Dise...mentioning

confidence: 99%

GABA_A receptors as targets for anaesthetics and analgesics and promising candidates to help treat coronavirus infections: A mini‐review

Luo

Balle

2022

Basic Clin Pharma Tox

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GABA is a major inhibitory neurotransmitter that regulates the balance between excitatory and inhibitory circuits in the human nervous system. The GABA receptors are divided into three main subtypes, GABA A , GABA B , and GABA C (also termed GABA A rho) receptors. GABA A receptors are pentameric ligand‐gated ion channels widely expressed throughout the central and peripheral nervous system. The activation of GABA A receptors results in opening of an anion‐selective channel that mainly gates chloride ions and allows them to flow into the neuron, causing hyperpolarization of the cell membrane that dampens neural excitability. This makes GABA A receptors critical anaesthetic and analgesic targets for existing as well as for the development of novel drugs. In this review, we first summarize the biochemical properties of GABA A receptors and the clinical anaesthetics and analgesics targeting the receptors. In a forward‐looking section, we summarize the emerging role of GABAergic signalling in treatment of COVID‐19 related infections. Finally, we discuss the opportunities arising from targeting specific and unique subunit interfaces for the development of novel anaesthetics and analgesics leading to more efficient therapies.

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“…The role of GABA in plants includes responding to abiotic and biotic stress factors, maintaining carbon/nitrogen (C/N) balance, and regulating plant development [ 4 ]. Furthermore, GABA shows a variety of physiological functions, including enhancing immunity [ 5 ], improving sleeplessness and depression [ 6 , 7 ], improving memory functions [ 8 ], regulating blood pressure [ 9 ], fighting obesity [ 10 ], and preventing diabetes [ 11 , 12 ]. Thus, in food and pharmaceuticals, GABA has the potential to be a bioactive ingredient.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Gamma-Aminobutyric Acid Production by Lactiplantibacillus plantarum FRT7 from Chinese Paocai

Cai

et al. 2023

Foods

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Gamma-aminobutyric acid (GABA) is a widely available non-protein amino acid whose physiological importance goes beyond its role as an inhibitory neurotransmitter in mammals. The GABA synthesis ability of ten strains of Lactiplantibacillus plantarum was screened. They produced GABA ranging from 48.19 ± 3.44 to 100.75 ± 1.63 mg/L at 24 h-cultivation. Among them, Lp. plantarum FRT7 showed the highest GABA production. Therefore, FRT7 was chosen for GABA yield optimization. A one-factor-at-a-time strategy analysis of the GABA yield of FRT7 was performed, including the culture temperature, incubation time, inoculum volume, initial pH, the initial amount of monosodium glutamate (MSG), and pyridoxal 5’-phosphate (PLP) concentration, based on which the response surface methodology (RSM) was performed. After being cultured in an MRS culture medium supplemented with 3% MSG and 2 mmol/L of PLP at 40 °C with an initial pH of 7.0 for 48 h, the GABA reached a maximum yield of 1158.6 ± 21.22 mg/L. The results showed the experimental value of the GABA yield was in good agreement with the predicted values. Furthermore, the results from the RSM also indicated that the initial MSG addition, PLP concentration, and incubation time were significant variables. These results suggest that Lp. plantarum FRT7 has the potential to be a health-beneficial probiotic with commercial capabilities.

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Sub-basal increases of GABA enhance the synthesis of TNF-α, TGF-β, and IL-1β in the immune system organs of the Nile tilapia

Cited by 8 publications

References 50 publications

Application of Biomimetic Cell-Derived Nanoparticles with Mannose Modification as a Novel Vaccine Delivery Platform against Teleost Fish Viral Disease

Application of Biomimetic Cell-Derived Nanoparticles with Mannose Modification as a Novel Vaccine Delivery Platform against Teleost Fish Viral Disease

GABA_A receptors as targets for anaesthetics and analgesics and promising candidates to help treat coronavirus infections: A mini‐review

Optimization of Gamma-Aminobutyric Acid Production by Lactiplantibacillus plantarum FRT7 from Chinese Paocai

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Sub-basal increases of GABA enhance the synthesis of TNF-α, TGF-β, and IL-1β in the immune system organs of the Nile tilapia

Cited by 8 publications

References 50 publications

Application of Biomimetic Cell-Derived Nanoparticles with Mannose Modification as a Novel Vaccine Delivery Platform against Teleost Fish Viral Disease

Application of Biomimetic Cell-Derived Nanoparticles with Mannose Modification as a Novel Vaccine Delivery Platform against Teleost Fish Viral Disease

GABAA receptors as targets for anaesthetics and analgesics and promising candidates to help treat coronavirus infections: A mini‐review

Optimization of Gamma-Aminobutyric Acid Production by Lactiplantibacillus plantarum FRT7 from Chinese Paocai

Contact Info

Product

Resources

About

GABA_A receptors as targets for anaesthetics and analgesics and promising candidates to help treat coronavirus infections: A mini‐review