Sub-attomolar electrochemical measurement of DNA hybridization based on the detection of high coverage biobarcode latex labels at PNA-modified screen printed electrodes

Widaningrum, Tarina; Widyastuti, Endrika; Pratiwi, Feby Wijaya; Fatimah, Ai Imas Faidoh; Rijiravanich, Patsamon; Somasundrum, Mithran; Surareungchai, Werasak

doi:10.1016/j.talanta.2017.01.094

Cited by 13 publications

(13 citation statements)

References 33 publications

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“…This review highlights the impact of the electrode material, electrode modification, and the detection technique in the process of the DNA based biosensor development. Sensitive and selective biosensors have been developed by many researchers (Widaningrum et al, 2017;Zhang et al, 2020). Many of these studies have used gold, ITO or carbon electrodes or magnetic nanoparticles.…”

Section: Discussion and Limitationsmentioning

confidence: 99%

“…This allows an approximate estimation of the required sensitivity in M concentration for the sensor to detect <100 CFU/mL in bacterial samples. For instance, Widaningrum et al (2017) detected 5.6x10 -19 M of synthetic DNA and 17 CFU/mL using STEC culture suggesting the LOD would need to reach zM to achieve such high sensitivity. A similar or lower LOD was only demonstrated by Ariffin et al (2020) and Ariffin et al (2018) among the selected literature.…”

Section: Discussion and Limitationsmentioning

confidence: 99%

“…Carbon is the most used ink for SPE which can then be additionally altered with other metals, polymers, or enzymes, depending on the application. An example for one such application has been reported Widaningrum et al (2017). Briefly, peptide nucleic acid (PNA) probe was immobilized onto the screen-printed carbon electrode (SPCE) by applying a potential of -0.5 V to working electrode for 300 seconds.…”

Section: Carbon Based Electrodesmentioning

confidence: 99%

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Electrochemical nucleic acid-based sensors for detection of E. coli and Shiga toxin-producing E. coli (STEC) – Review of the recent developments

Wasiewska

Seymour

Juska

et al. 2022

Preprint

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Escherichia coli (E. coli) are a group of bacteria, which are a natural part of the intestinal flora of warm-blooded animals, including humans. Most E. coli are non-pathogenic and are essential for the normal function of a healthy intestine, however, certain types such as Shiga toxin-producing E. coli (STEC) is a foodborne pathogen that can cause a life-threatening illness. Development of point-of-care (POC), rapid detection devices for E. coli have high importance from a food safety point of view. The most suitable way to distinguish between generic E. coli and STEC is by using nucleic acid-based detection, focusing on the virulence factors. Electrochemical sensors based on nucleic acid recognition have attracted much attention in recent years for use in pathogenic bacteria detection. This review has summarised nucleic acid-based sensors for the detection of generic E. coli and STEC since 2015. First, the sequences of the genes used as recognition probes are discussed and compared to the most recent research regarding the specific detection of general E. coli and STEC. Subsequently, the collected literature regarding nucleic acid-based sensors was described and discussed. The traditional sensors were divided into four categories such as gold, ITO, carbon-based electrodes, and those using magnetic particles. Finally, we summarised the future trends in nucleic acid-based sensor development for E. coli and STEC including some examples of fully integrated devices

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Section: Discussion and Limitationsmentioning

confidence: 99%

Section: Discussion and Limitationsmentioning

confidence: 99%

Section: Carbon Based Electrodesmentioning

confidence: 99%

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Electrochemical nucleic acid-based sensors for detection of E. coli and Shiga toxin-producing E. coli (STEC) – Review of the recent developments

Wasiewska

Seymour

Juska

et al. 2022

Preprint

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“…217 SPE using gold and carbon has been employed as electrochemical biosensor electrodes for the detection of human gene, bacteria, miRNA, and DNA. 49,69,101,218 IDEs are fabricated using two interdigitated comb-like microelectrodes with periodically systemized gaps and leg. 219 The advantages of the structure include flexibility in adjusting the size of the gap to accommodate the biorecognition assembly and the wide variety of analytes including very small molecules to viruses and pathogens that can be detected.…”

Section: Electrical and Electrochemical Transducersmentioning

confidence: 99%

Attomolar analyte sensing techniques (AttoSens): a review on a decade of progress on chemical and biosensing nanoplatforms

et al. 2021

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“…Among these techniques, DNA biosensors constitute an important place due to their advantage of recognizing base pairs efficiently in shorter time values compared to laborious classical methods. Biosensors that use electrochemical transducers have been used commonly in order to construct sensitive, selective, inexpensive, rapid, simple and miniaturized biosensing platforms .…”

Section: Introductionmentioning

confidence: 99%

Poly‐L‐lysine Coated Surfaces for Ultrasensitive Nucleic Acid Detection

2018

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Poly‐L‐lysine is one of the biocompatible polymers having amino and carboxyl groups in its structure. This attractive feature of poly‐L‐lysine makes it very convenient for bioactive molecule attachment. This study details the preparation of poly‐L‐lysine‐based pencil graphite electrodes (PLL/PGEs) and use of the coated electrodes for direct ultrasensitive DNA hybridization detection. In the first part of this study, poly‐L‐lysine coated electrodes were prepared using L‐lysine as the monomer by cyclic voltammetry (CV) with different cyclic scans. The effect of these cyclic scans during the electropolymerization was investigated. Coated electrodes were characterized by cyclic voltammetry, electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM). Then, one‐pot preparation of poly‐L‐lysine composites with graphene (GN) and multi‐walled carbon nanotubes (MWCNTs) onto the pencil graphite electrodes were achieved. Electrochemical responses of these 3 electrodes were compared. After all, electrochemical DNA hybridization was performed using the poly‐L‐lysine‐based electrodes prepared at optimum polymerization condition. The PLL/PGE coated electrode presented a good linear response in the target concentration range of 1.0×10−13 to 1.0×10−6 with a detection limit of 2.25×10−14 using differential pulse voltammetry as the detection method. We believe that poly‐L‐lysine‐based surfaces will be useful for further clinical applications.

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Sub-attomolar electrochemical measurement of DNA hybridization based on the detection of high coverage biobarcode latex labels at PNA-modified screen printed electrodes

Cited by 13 publications

References 33 publications

Electrochemical nucleic acid-based sensors for detection of E. coli and Shiga toxin-producing E. coli (STEC) – Review of the recent developments

Electrochemical nucleic acid-based sensors for detection of E. coli and Shiga toxin-producing E. coli (STEC) – Review of the recent developments

Attomolar analyte sensing techniques (AttoSens): a review on a decade of progress on chemical and biosensing nanoplatforms

Poly‐L‐lysine Coated Surfaces for Ultrasensitive Nucleic Acid Detection

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