SU-8-based nanoporous substrate for migration of neuronal cells

Kim, Eun‐Hee; Yoo, Sung Jin; Moon, Cheil; Nelson, Bradley J.; Choi, Hongsoo

doi:10.1016/j.mee.2015.03.016

Cited by 6 publications

(15 citation statements)

References 28 publications

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“…Therefore, we use this size to enhance neurite outgrowth on the nanostructured SU-8 microprobe. We successfully applied the nanostructuring technique to the surface of the SU-8 microprobe and obtained nanostructures that were consistent with our previous study [17].…”

Section: Fabrication Of Su-8-based Microprobessupporting

confidence: 85%

“…The size of cultured neuronal cells has cell bodies with a diameter of 15-20 μm [25,26]. Cells can thus be attached to the surface of the nanostructured SU-8 microprobe compared to the non-nanostructured SU-8 microprobe, according to our previous study [17,18]. In addition, cells could be induced to lead to electrode sites by the pathway as they adhere to the microprobes.…”

Section: Fabrication Of Su-8-based Microprobesmentioning

confidence: 92%

“…We used SU-8 because of its biocompatibility and suitability for neural interface implants [19,20]. We can apply the proposed procedure to the preparation of planar microprobes, as discussed in our previous work [17]. Therefore, the microprobes were prepared by conventional photolithography and the nanostructures on their surface were then realized by nanospheric lithography (NSL) [21,22].…”

Section: Open Accessmentioning

confidence: 99%

“…Although neuronal cells prefer a nanostructured surface, the majority of flexible microprobes have a flat surface. In our previous work, a nanostructured SU-8 substrate exhibited similar neuronal outgrowth to a flat SU-8 surface coated with poly-l-lysine (PLL), which facilitates adhesion by rat pheochromocytoma (PC12) cells [17,18]. These findings motivated us to develop SU-8-based microprobes with nanostructures to support neuronal cells on their surface over a long period of time.…”

Section: Introductionmentioning

confidence: 96%

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An SU-8-based microprobe with a nanostructured surface enhances neuronal cell attachment and growth

Kim

Choi

2017

Micro and Nano Syst Lett

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Microprobes are used to repair neuronal injury by recording electrical signals from neuronal cells around the surface of the device. Following implantation into the brain, the immune response results in formation of scar tissue around the microprobe. However, neurons must be in close proximity to the microprobe to enable signal recording. A common reason for failure of microprobes is impaired signal recording due to scar tissue, which is not related to the microprobe itself. Therefore, the device-cell interface must be improved to increase the number of neurons in contact with the surface. In this study, we developed nanostructured SU-8 microprobes to support neuronal growth. Nanostructures of 200 nm diameter and depth were applied to the surface of microprobes, and the attachment and neurite outgrowth of PC12 cells on the microprobes were evaluated. Neuronal attachment and neurite outgrowth on the nanostructured microprobes were significantly greater than those on non-nanostructured microprobes. The enhanced neuronal attachment and neurite outgrowth on the nanostructured microprobes occurred in the absence of an adhesive coating, such as poly-l-lysine, and so may be useful for implantable devices for long-term use. Therefore, nanostructured microprobes can be implanted without adhesive coating, which can cause problems in vivo over the long term.

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Section: Fabrication Of Su-8-based Microprobessupporting

confidence: 85%

Section: Fabrication Of Su-8-based Microprobesmentioning

confidence: 92%

Section: Open Accessmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations

An SU-8-based microprobe with a nanostructured surface enhances neuronal cell attachment and growth

Kim

Choi

2017

Micro and Nano Syst Lett

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“…SU-8 surfaces with nanopores have also been fabricated and investigated for enhanced cell attachment. Kim et al utilized a combination of polystyrene (PS) nanoparticles and patterned chromium (Cr) layer as an etching mask to create nanopores in SU-8 [ 50 ]. Figure 5 shows the SEM images of the fabrication processes for the nanoporous SU-8 substrate.…”

Section: Applicationsmentioning

confidence: 99%

Biocompatibility of SU-8 and Its Biomedical Device Applications

Chen

Lee

2021

Micromachines

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SU-8 is an epoxy-based, negative-tone photoresist that has been extensively utilized to fabricate myriads of devices including biomedical devices in the recent years. This paper first reviews the biocompatibility of SU-8 for in vitro and in vivo applications. Surface modification techniques as well as various biomedical applications based on SU-8 are also discussed. Although SU-8 might not be completely biocompatible, existing surface modification techniques, such as O2 plasma treatment or grafting of biocompatible polymers, might be sufficient to minimize biofouling caused by SU-8. As a result, a great deal of effort has been directed to the development of SU-8-based functional devices for biomedical applications. This review includes biomedical applications such as platforms for cell culture and cell encapsulation, immunosensing, neural probes, and implantable pressure sensors. Proper treatments of SU-8 and slight modification of surfaces have enabled the SU-8 as one of the unique choices of materials in the fabrication of biomedical devices. Due to the versatility of SU-8 and comparative advantages in terms of improved Young’s modulus and yield strength, we believe that SU-8-based biomedical devices would gain wider proliferation among the biomedical community in the future.

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Poly-l-lactic acid-co-poly(pentadecalactone) Electrospun Fibers Result in Greater Neurite Outgrowth of Chick Dorsal Root Ganglia in Vitro Compared to Poly-l-lactic Acid Fibers

Ziemba

Lane

Segundo

et al. 2018

ACS Biomater. Sci. Eng.

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Electrospun poly-l-lactic acid (PLLA) fiber scaffolds are used to direct axonal extension in neural engineering models. We aimed to improve the efficacy of these fibers in promoting neurite outgrowth by altering surface topography and reducing fiber elastic modulus through the incorporation of a compatibilized blend, poly-l-lactic acid-poly(pentadecalactone) (PLLA–PPDL) into the solution prior to electrospinning. PLLA+PLLA–PPDL fibers had a larger diameter, increased surface nanotopography, and lower glass transition temperature than PLLA fibers but had similar mechanical properties. Increases in neurite outgrowth on PLLA+PLLA–PPDL fibers were observed, potentially due to the significantly increased diameter and surface coverage with nanotopography. Ultimately, these results suggest that greater electrospun fiber diameter and surface topography may contribute to increases in neurite outgrowth.

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SU-8-based nanoporous substrate for migration of neuronal cells

Cited by 6 publications

References 28 publications

An SU-8-based microprobe with a nanostructured surface enhances neuronal cell attachment and growth

An SU-8-based microprobe with a nanostructured surface enhances neuronal cell attachment and growth

Biocompatibility of SU-8 and Its Biomedical Device Applications

Poly-l-lactic acid-co-poly(pentadecalactone) Electrospun Fibers Result in Greater Neurite Outgrowth of Chick Dorsal Root Ganglia in Vitro Compared to Poly-l-lactic Acid Fibers

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