Styrene impairs normal embryo development in the Mediterranean mussel (Mytilus galloprovincialis)

Wathsala, Rajapaksha Haddokara Gedara Rasika; Franzellitti, Silvia; Scaglione, Morena; Fabbri, Elena

doi:10.1016/j.aquatox.2018.05.026

Cited by 20 publications

(9 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Applying Kohinor and Calypso resulted in an accumulation increase of rhodamine B in isolated gill tissues, similarly to the dose-dependent enhancement of rhodamine concentration in the presence of the MXR inhibitor verapamil (Cornwall et al, 1995). Very recently Wathsala, et al (2018) suggested reduced MXR activity by higher rhodamine accumulation following an identical co-incubation protocol of styrene on treated mussel embryos.…”

Section: Resultsmentioning

confidence: 73%

Neonicotinoid insecticides are potential substrates of the multixenobiotic resistance (MXR) mechanism in the non-target invertebrate, Dreissena sp.

et al. 2018

View full text Add to dashboard Cite

Mussels are among the most frequently used invertebrate animals in aquatic toxicology to detect toxic exposure in the environment. The presence and activity of a cellular defence system, the multixenobiotic resistance (MXR) mechanism, was also established in these organisms.In isolated gill tissues of dreissenid mussels (D. bugensis) the MXR activity was assayed after treatment by commercially available insecticides (formulated products) which contain neonicotinoids as their active ingredients: Actara (thiamethoxam), Apacs (clothianidin), Calypso (thiacloprid) and Kohinor (imidacloprid), respectively. While applying the accumulation assay method, 0.5 μM rhodamine B was used as model substrate and 20 μM verapamil as model inhibitor of the MXR mechanism.In acute (in vitro) experiments when isolated gills were co-incubated in graded concentrations of insecticides and rhodamine B simultaneously, Calypso and Kohinor treatment resulted increasing rhodamine accumulation. Chemical analysis of gills in vitro incubated in insecticides demonstrated higher tissue concentrations of thiamethoxam, clothianidin and thiacloprid in the presence of verapamil suggesting that the active ingredients of Actara, Apacs and Calypso are potential substrates of the MXR mediated cellular efflux. In contrast, verapamil did significantly alter the accumulated imidacloprid concentrations in gills, suggesting that the active component of Kohinor is not transported by the MXR mechanism.Chronic (in vivo) exposures of the intact animals in lower, 1, 10 mg/L concentration of neonicotinoid products, resulted in a decreased level of both rhodamine accumulation and verapamil inhibition by the 12 th -14 th days of treatment. These results suggest an enhancement of MXR activity (chemostimulation), building up gradually in the animals exposed to Actara, Apacs and Kohinor, respectively.Neonicotinoid-type insecticides are generally considered as selective neurotoxins for insects, targeting the nicotinic type acetylcholine receptors (nAChRs) in their central nervous system. Our present results provide the first evidences that neonicotinoid insecticides are also able to alter the transmembrane transport mechanisms related to the MXR system.

show abstract

Section: Resultsmentioning

confidence: 73%

Neonicotinoid insecticides are potential substrates of the multixenobiotic resistance (MXR) mechanism in the non-target invertebrate, Dreissena sp.

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The most abundant polymers found at the deepest site investigated here, the SIS, is a copolymer employed as an impact modifier and toughener in plastics and (structural) adhesives (https://polymerdatabase.com) and is widely used in sealants, gasket materials, rubber bands, toy products, shoe soles, bitumen products for road paving and antifouling paints. Although SIS has been found in the intestines of deep-sea benthic organisms, suggesting its potential elimination from the organism (Cau et al, 2020), at the same time the negative effects of styrene on marine organisms have been documented (Mamaca et al, 2005;Wathsala et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Extraction efficiency of different microplastic polymers from deep-sea sediments and their quantitative relevance

Canensi

Barucca²,

Corinaldesi³

2022

Front. Mar. Sci.

View full text Add to dashboard Cite

An increasing number of methods for extracting microplastic particles from marine sediments have been published but without evaluating the extraction efficiency. Furthermore, while most of the procedures developed have been applied to sandy sediments from shallow water habitats, specific and standardized procedures for deep-water sediments (> 200 meters deep) are limited. In this study, we describe a specific protocol for extracting microplastics (2- 1000 µm) from deep-sea sediments and for quantifying and identifying them. We also assessed its extraction efficiency, which resulted in a high recovery (on average ca. 60%, and up to 80%) particularly, for polyethylene, polypropylene, and polystyrene. This method can be applied to all fine-grained/muddy sediments and allows the extraction of even the smallest fraction of microplastics (<20 µm), which are expected to have the most severe effects on marine biodiversity and ecosystem functioning and ultimately also have implications for human health.

show abstract

“…SOs could pose a higher risk to marine biota, given that SOs in seawater are in many times larger orders of magnitude than HBCDs, and SM bioassays reach toxicological endpoints at lower concentrations. According to Wathsala et al (2018), a significant reduction in normal M. galloprovincialis larvae development, including malformation, is induced from 0.1 μg/L (LOEC), and decreasing at higher concentrations. SOs concentrations in seawater around the globe surpass the LOEC value in most countries (Table S1).…”

Section: Discussionmentioning

confidence: 99%

“…A further study found that a 7 days continuous exposure of 0.2 mg/L SM to mussel Mytilus edulis and fish Symphodus melops disrupts lysosomal membrane stability and leads to DNA damage in blood cells and haemocyte (Mamaca et al, 2005). Importantly, SM at relatively realistic concentrations impacts on the development of mussel Mytilus galloprovincialis at early life stages, including transcriptional effects on the genes involved in shell biogenesis (Wathsala et al, 2018), a crucial process for assuring the survival in their adult stages. To the best of our knowledge, the toxicity of SD and ST have not been assessed before.…”

Section: Ecotoxicitymentioning

confidence: 97%

See 1 more Smart Citation

Global distribution of two polystyrene-derived contaminants in the marine environment: A review

De-la-Torre

Dioses-Salinas

Pizarro-Ortega

et al. 2020

Marine Pollution Bulletin

View full text Add to dashboard Cite

Plastic pollution is one of the major issues impacting on the marine environment. Plastic polymers are known to leach industrial chemicals and associated contaminants. In this review, we focused on assessing the global distribution and concentration of two polystyrene-derived contaminants, hexabromocyclododecanes (HBCDs) and styrene oligomers (SOs), in marine sediments and seawater. Overall, most of the studies were carried out in Asia, North America, and Europe. Relatively high concentrations of these contaminants are generally attributed to the proximity of urban cities, plastic industries, polystyrene pollution, and aquaculture. Moreover, the concentrations in sediments are many times higher than in seawater. HBCDs were found to be a negligible risk to marine biota when compared to the ecotoxicological endpoints. However, realistic concentrations of SOs could compromise the wellbeing of certain species in highly polluted sites. The future perspectives and research were discussed.

show abstract

Styrene impairs normal embryo development in the Mediterranean mussel (Mytilus galloprovincialis)

Cited by 20 publications

References 66 publications

Neonicotinoid insecticides are potential substrates of the multixenobiotic resistance (MXR) mechanism in the non-target invertebrate, Dreissena sp.

Neonicotinoid insecticides are potential substrates of the multixenobiotic resistance (MXR) mechanism in the non-target invertebrate, Dreissena sp.

Extraction efficiency of different microplastic polymers from deep-sea sediments and their quantitative relevance

Global distribution of two polystyrene-derived contaminants in the marine environment: A review

Contact Info

Product

Resources

About