Stylopine fromChelidonium majus inhibits LPS-Induced inflammatory mediators in RAW 264.7 cells

Abstract

Stylopine is a major component of the leaf of Chelidonium majus L. (Papaveraceae), which has been used for the removal of warts, papillomas and condylomas, as well as the treatment of liver disease, in oriental countries. Stylopine per se had no cytotoxic effect in unstimulated RAW 264.7 cells, but concentration-dependently reduced nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta), and the IL-6 production and cyclooxygenase-2 (COX-2) activity caused by the LPS stimulation. The levels of inducible nitric oxide synthase (iNOS) and COX-2 protein expressions were markedly suppressed by stylopine in a concentration dependent manner. These results suggest that stylopine suppress the NO and PGE2 production in macrophages by inhibiting the iNOS and COX-2 expressions. These biological activities of stylopine may contribute to the anti-inflammatory activity of Chelidonium majus.

Lycorine inhibits lipopolysaccharide-induced iNOS and COX-2 up-regulation in RAW264.7 cells through suppressing P38 and STATs activation and increases the survival rate of mice after LPS challenge

“…Moreover, new antiinflammatory drugs are being discovered and developed based on their effects on signal transduction and as anti-cytokine agents [30]. Previous studies have revealed that LPS-induced inflammatory mediator production by macrophages can be attenuated by isoquinoline alkaloids like berberine, and stylopine [31,32]. Lycorine is one of the most common isoquinoline alkaloids and has a variety of pharmacological activities [16][17][18][19].…”

Lipopolysaccharide induces calcitonin gene‐related peptide in the RAW264.7 macrophage cell line

“…Lipopolysaccharide has been reported to up‐regulate the expression of inflammatory mediators such as IL‐1β, tumour necrosis factor‐α (TNFα), IL‐6, nerve growth factor (NGF), inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2)/prostaglandin E 2 (PGE 2 ) in RAW264.7 macrophages 11–13 . The nuclear factor‐κB (NF‐κB) signalling pathway is involved in LPS‐induced production of inflammatory mediators in this cell line 12,14 .…”

“…Lipopolysaccharide has been reported to up-regulate the expression of inflammatory mediators such as IL-1b, tumour necrosis factor-a (TNFa), IL-6, nerve growth factor (NGF), inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2)/prostaglandin E 2 (PGE 2 ) in RAW264.7 macrophages. [11][12][13] The nuclear factor-jB (NF-jB) signalling pathway is involved in LPS-induced production of inflammatory mediators in this cell line. 12,14 As a transcription factor, NF-jB plays a key role in the transcriptional regulation of genes of numerous inflammatory mediators including iNOS, COX2, IL-1b, IL-6 and TNFa.…”

Biocatalytic Organic Synthesis of Optically Pure (S)-Scoulerine and Berbine and Benzylisoquinoline Alkaloids

“…Both alkaloid families are associated with a broad range of biological activities: Many 1-benzyl-1,2,3,4-tetrahydroisoquinolines act as antispasmodic or hypotensive and some, such as norcoclaurine, coclaurine, and N -methylcoclaurine, possess anti-HIV activity in vitro . (2) Berbines show diverse biological activities such as analgesic, sedative, hypnotic, or anti-inflammatory effects,(3) and the non-natural derivative l -chloroscoulerine is currently investigated as a novel treatment of schizophrenia. (4) In addition, tetrahydroisoquinolines have recently been employed as chiral ligands for metal-catalyzed transfer-hydrogenation.…”