Studying the fate of tumor extracellular vesicles at high spatio-temporal resolution using the zebrafish embryo

Hyenne, Vincent; Ghoroghi, Shima; Collot, Mayeul; Harlepp, Sébastien; Bauer, Jack; Mercier, Luc; Busnelli, Ignacio; Fekonja, Nina; Machado, Pedro; Bons, Joanna; Delalande, François; Amor, Ana Isabel; García‐Silva, Susana; Verweij, Frederik J.; Niel, Guillaume van; Schwab, Yannick; Peinado, Héctor; Carapito, Christine; Klymchenko, Andrey S.; Goetz, Jacky G.

doi:10.1101/380238

Cited by 2 publications

(2 citation statements)

References 89 publications

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“…Circulating tumor EVs were also shown to be internalized by endothelial cells and patrolling macrophages. So far, these observations were made in zebrafish embryos and remains to be tested in mice …”

Section: Cell Fate Mapping Imaging Molecular Activities and Cell‐cementioning

confidence: 99%

“…So far, these observations were made in zebrafish embryos and remains to be tested in mice. 91 A novel type of distant cell-cell communication was identified in brain tumor cells that were shown to interconnect using long membrane protrusions (LMPs) or microtubes forming a multicellular network ( Figure 2E). 60…”

Section: Direct and Indirect Interactions Between Cancer Cells: Extmentioning

confidence: 99%

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Frontiers in intravital multiphoton microscopy of cancer

2019

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Background Cancer is a highly complex disease, which involves the cooperation of tumor cells with multiple types of host cells and the extracellular matrix. Cancer studies that rely solely on static measurements of individual cell types are insufficient to dissect this complexity. In the last two decades, intravital microscopy has established itself as a powerful technique that can significantly improve our understanding of cancer by revealing the dynamic interactions governing cancer initiation, progression, and treatment effects in living animals. This review focuses on intravital multiphoton microscopy (IV‐MPM) applications in mouse models of cancer. Recent findings IV‐MPM studies have already enabled a deeper understanding of the complex events occurring in cancer at the molecular, cellular, and tissue levels. Multiple cell types present in different tissues influence cancer cell behavior via activation of distinct signaling pathways. As a result, the boundaries in the field of IV‐MPM are continuously being pushed to provide an integrated comprehension of cancer. We propose that optics, informatics, and cancer (cell) biology are coevolving as a new field. We have identified four emerging themes in this new field. First, new microscopy systems and image processing algorithms are enabling the simultaneous identification of multiple interactions between the tumor cells and the components of the tumor microenvironment. Second, techniques from molecular biology are being exploited to visualize subcellular structures and protein activities within individual cells of interest and relate those to phenotypic decisions, opening the door for “in vivo cell biology”. Third, combining IV‐MPM with additional imaging modalities or omics studies holds promise for linking the cell phenotype to its genotype, metabolic state, or tissue location. Finally, the clinical use of IV‐MPM for analyzing efficacy of anticancer treatments is steadily growing, suggesting a future role of IV‐MPM for personalized medicine. Conclusion IV‐MPM has revolutionized visualization of tumor‐microenvironment interactions in real time. Moving forward, incorporation of novel optics, automated image processing, and omics technologies in the study of cancer biology, will not only advance our understanding of the underlying complexities but will also leverage the unique aspects of IV‐MPM for clinical use.

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Section: Cell Fate Mapping Imaging Molecular Activities and Cell‐cementioning

confidence: 99%

Section: Direct and Indirect Interactions Between Cancer Cells: Extmentioning

confidence: 99%

Frontiers in intravital multiphoton microscopy of cancer

2019

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Extracellular vesicles – developmental messengers of tissue crosstalk

Witte¹,

Gross²

2019

TEV

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Extracellular vesicles are membrane particles secreted from cells into all body fluids. They convey signals that induce long-term changes in cellular behavior. During development, EV secretion and the specific loading of signalling factors in EVs contributes to organ development and tissue differentiation. Different biomolecules such as proteins, lipids and nucleic acids transmit these signals and the content, size, and membrane composition of EVs are highly dynamic and depend on the cellular source, state, and environmental conditions.Here we review current in vivo studies in different model organisms regarding extracellular vesicles and their physiological role in the formation of cell types, tissues and refined body structures.

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Studying the fate of tumor extracellular vesicles at high spatio-temporal resolution using the zebrafish embryo

Cited by 2 publications

References 89 publications

Frontiers in intravital multiphoton microscopy of cancer

Frontiers in intravital multiphoton microscopy of cancer

Extracellular vesicles – developmental messengers of tissue crosstalk

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