Studying the effects of genistein on gene expression of fish embryos as an alternative testing approach for endocrine disruption

Schiller, Viktoria; Wichmann, Arne; Kriehuber, Ralf; Muth-Köhne, Elke; Giesy, John P.; Hecker, Markus; Fenske, Martina

doi:10.1016/j.cbpc.2012.09.005

Cited by 35 publications

(27 citation statements)

References 83 publications

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“…Studies include very early exposures immediately after fertilization (e.g., Hermsen et al , 2012; Schiller et al , 2013b), as well as exposures of stages after hatching of the embryo (e.g., Driessen et al , 2015; Faltermann et al , 2014; Hahn et al , 2014; Kawabata et al , 2015). The exposure durations cover a range between 1 h (Alexeyenko et al , 2010) to several days (e.g., Garcia-Reyero et al , 2014; Lam et al , 2011; Park et al , 2012).…”

Section: Resultsmentioning

confidence: 99%

The Transcriptome of the Zebrafish Embryo After Chemical Exposure: A Meta-Analysis

Schüttler

Reiche

Altenburger

et al. 2017

Toxicological Sciences

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Numerous studies have been published in the past years investigating the transcriptome of the zebrafish embryo (ZFE) upon being subjected to chemical stress. Aiming at a more mechanistic understanding of the results of such studies, knowledge about commonalities of transcript regulation in response to chemical stress is needed. Thus, our goal in this study was to identify and interpret genes and gene sets constituting a general response to chemical exposure. Therefore, we aggregated and reanalyzed published toxicogenomics data obtained with the ZFE. We found that overlap of differentially transcribed genes in response to chemical stress across independent studies is generally low and the most commonly differentially transcribed genes appear in less than 50% of all treatments across studies. However, effect size analysis revealed several genes showing a common trend of differential expression, among which genes related to calcium homeostasis emerged as key, especially in exposure settings up to 24 h post-fertilization. Additionally, we found that these and other downregulated genes are often linked to anatomical regions developing during the respective exposure period. Genes showing a trend of increased expression were, among others, linked to signaling pathways (e.g., Wnt, Fgf) as well as lysosomal structures and apoptosis. The findings of this study help to increase the understanding of chemical stress responses in the developing zebrafish embryo and provide a starting point to improve experimental designs for this model system. In future, improved time- and concentration-resolved experiments should offer better understanding of stress response patterns and access to mechanistic information.

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Section: Resultsmentioning

confidence: 99%

The Transcriptome of the Zebrafish Embryo After Chemical Exposure: A Meta-Analysis

Schüttler

Reiche

Altenburger

et al. 2017

Toxicological Sciences

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show abstract

“…The evaluation of sets of genes by qPCR is considered to be a promising strategy for endocrine disruption investigation, when following optimized experimental design regarding exposure intervals and evaluated zebrafish developmental stages [64]. The transgenic zebrafish embryos were also considered to be experimental models compatible with EDA, and their integration in future studies is expected to be facilitated by automated image analysis procedures [52].…”

Section: Estrogenicity Assessment By Gene Expressionmentioning

confidence: 99%

The value of zebrafish as an integrative model in effect-directed analysis - a review

et al. 2015

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“…For this purpose, all lethal and non-lethal morphological effects in the embryos were assessed after 24, 48 and 96 h (zebrafish and medaka) and after 7 d (medaka only), as described in detail in Schiller et al, 2013a. From the resulting concentration-response curves (data not shown), the 48 h (zebrafish) and 7 d (medaka) cumulative lethal and non-lethal effect concentrations and EC 20 values were derived after probit analysis (ToxRat ® Professional 2.10).…”

Section: Fish Embryo Toxicity Testsmentioning

confidence: 99%

Species-specific considerations in using the fish embryo test as an alternative to identify endocrine disruption

Schiller¹,

Zhang²,

Hecker³

et al. 2014

Aquatic Toxicology

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Studying the effects of genistein on gene expression of fish embryos as an alternative testing approach for endocrine disruption

Cited by 35 publications

References 83 publications

The Transcriptome of the Zebrafish Embryo After Chemical Exposure: A Meta-Analysis

The Transcriptome of the Zebrafish Embryo After Chemical Exposure: A Meta-Analysis

The value of zebrafish as an integrative model in effect-directed analysis - a review

Species-specific considerations in using the fish embryo test as an alternative to identify endocrine disruption

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