Studying the effect of graphene-ZnO nanocomposites on polymerase chain reaction

Sharma, Vinay; Rajaura, Rajveer Singh; Sharma, Preetam K.; Srivastava, Rishabh Ronin; Sharma, Shyam Sundar; Agrawal, Kailash

doi:10.1063/1.4946577

Cited by 1 publication

(1 citation statement)

References 8 publications

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“…Some of the organic and biological reagents used as additives include formamide 6 , dimethyl sulfoxide (DMSO) 7 , tetramethylammonium chloride 8 , and single-stranded binding protein 1 . More recently, researchers have identified numerous nanomaterials that can enhance the yield of PCR, such as silver nanoparticles 9 , gold nanoparticles 9 , 10 , magnetic nanoparticles 9 , TiO 2 nanoparticles 10 , carbon-coated silica nanoparticles 11 , and graphene-ZnO nanocomposites 12 . Nanomaterials can also improve the specificity of PCR, including gold nanoparticles 13 , poly(diallyldimethylammonium chloride)-protected gold nanoparticles 14 , dendrimers 15 , amino-modified magnetic nanoparticles 16 , and graphene oxide 17 .…”

Section: Introductionmentioning

confidence: 99%

Enhancement of PCR Sensitivity and Yield Using Thiol-modified Primers

Bai

Xiao

Suo

et al. 2018

Sci Rep

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Various additives can enhance the quality of PCR amplification, but these generally require considerable optimization to achieve peak performance. Here, we demonstrate that the use of thiol-modified primers can enhance both PCR sensitivity and yield. In experiments with V. parahaemolyticus genomic DNA, this primer modification enhances PCR sensitivity by more than 100-fold, with accompanying improvements in amplicon yield. Then, an artificial plasmid with the same primer binding regions and different internal amplification sequence was designed. The result showed that the amplification also be improved by using the same thiol-modified primers. It indicated the enhancement was not caused by the effect of the thiol-modified primers on the second structure of amplification sequence. Subsequent experiments demonstrate that the effects of this modification are potentially due to altered interaction between the primers and proteins in the reaction mixture. Amplification with thiol-modified primers was strongly inhibited by the presence of extraneous proteins relative to standard DNA primers, which indicates that thiol-modified primers may be inhibited due to interaction with these proteins. In contaminant-free reactions, however, the thiol-modified primers might interact more strongly with DNA polymerase, which could in turn improve PCR amplification.

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