Studying pharmacodynamic effects in cell cultures by chemical fingerprinting − SIA electronic tongue versus 2D fluorescence soft sensor

Zabadaj, Marcin; Szuplewska, Aleksandra; Kalinowska, Dominika; Chudy, M.; Ciosek-Skibińska, Patrycja

doi:10.1016/j.snb.2018.05.137

Cited by 13 publications

(14 citation statements)

References 37 publications

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“…A similar tendency (apoptosis triggering) was also noted in the case of melanoma cells treated with both types of tested nanomaterials. The dysfunction of proliferative potential and apoptotic cell death is proposed as one of the most desired mechanisms of action in anticancer treatment [41]. To propose a potential mechanism of 2D Ti 3 C 2 cytotoxicity (in both Pristine and PLL modified forms), we tested their influence on DNA synthesis after exposure to the highest applied concentration, which was toxic towards both skin cell lines.…”

Section: Analysis Of Potential Cytotoxicitymentioning

confidence: 99%

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Engineering of 2D Ti3C2 MXene Surface Charge and its Influence on Biological Properties

Rozmysłowska-Wojciechowska

Mitrzak

Szuplewska

et al. 2020

Materials

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Current trends in the field of MXenes emphasize the importance of controlling their surface features for successful application in biotechnological areas. The ability to stabilize the surface properties of MXenes has been demonstrated here through surface charge engineering. It was thus determined how changing the surface charges of two-dimensional (2D) Ti3C2 MXene phase flakes using cationic polymeric poly-L-lysine (PLL) molecules affects the colloidal and biological properties of the resulting hybrid 2D nanomaterial. Electrostatic adsorption of PLL on the surface of delaminated 2D Ti3C2 flakes occurs efficiently, leads to changing an MXene’s negative surface charge toward a positive value, which can also be effectively managed through pH changes. Analysis of bioactive properties revealed additional antibacterial functionality of the developed 2D Ti3C2/PLL MXene flakes concerning Escherichia. coli Gram-negative bacteria cells. A reduction of two orders of magnitude of viable cells was achieved at a concentration of 200 mg L−1. The in vitro analysis also showed lowered toxicity in the concentration range up to 375 mg L−1. The presented study demonstrates a feasible approach to control surface properties of 2D Ti3C2 MXene flakes through surface charge engineering which was also verified in vitro for usage in biotechnology or nanomedicine applications.

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Section: Analysis Of Potential Cytotoxicitymentioning

confidence: 99%

Section: Analysis Of Potential Cytotoxicitymentioning

confidence: 99%

Engineering of 2D Ti3C2 MXene Surface Charge and its Influence on Biological Properties

Rozmysłowska-Wojciechowska

Mitrzak

Szuplewska

et al. 2020

Materials

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“…For further data analysis each matrix was unfolded to a vector of 3915 features (missing data, i.e., white areas on EEMs, Figure 1, were omitted). Similar strategy we applied in our previous research on 2D fluorescence [15,16].…”

Section: Methodsmentioning

confidence: 99%

Quantum Dots—Assisted 2D Fluorescence for Pattern Based Sensing of Amino Acids, Oligopeptides and Neurotransmitters

Zabadaj¹,

Ciosek-Skibińska²

2019

Sensors

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Quantum dots (QDs) are very attractive nanomaterials for analytical chemistry, due to high photostability, large surface area featuring numerous ways of bioconjugation with biomolecules, usually high quantum yield and long decay times. Their broad absorption spectra and narrow, sharp emission spectra of size-tunable fluorescence make them ideal tools for pattern-based sensing. However, almost always they are applied for specific sensing with zero-dimensional (0D) signal reporting (only peak heights or peak shifts are considered), without taking advantage of greater amount of information hidden in 1D signal (emission spectra), or huge amount of information hidden in 2D fluorescence maps (Excitation-Emission Matrixes, EEMs). Therefore, in this work we propose opposite strategy—non-specific interactions of QDs, which are usually avoided and regarded as their disadvantage, were exploited here for 2D fluorescence fingerprinting. Analyte-specific multivariate fluorescence response of QDs is decoded with the use of Partial Least Squares—Discriminant Analysis. Even though only one type of QDs is studied, the proposed pattern-based method enables to obtain satisfactory accuracy for all studied compounds—various neurotransmitters, amino-acids and oligopeptides. This is a proof of principle of the possibility of the identification of various bioanalytes by such fluorescence fingerprinting with the use of QDs.

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“…It is performed by transforming the obtained results into so-called latent components enabling the calculation of the dependent variables [62]. We applied this data analysis technique for various applications of the electronic tongue system [63][64][65]. In this paper, electronic tongue signals in appropriate time points were used for PLS modeling.…”

Section: Electronic Tongue-prediction Of Dissolution Studymentioning

confidence: 99%

Utilization of Ethylcellulose Microparticles with Rupatadine Fumarate in Designing Orodispersible Minitablets with Taste Masking Effect

et al. 2020

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Minitablets in orodispersible form constitute a flexible drug delivery tool for paediatric and geriatric population as they eliminate the risk of chocking and do not require drinking water in the application. Due to their direct contact with taste buds, taste sensation is an important factor. Preparing microparticles with taste masking polymers utilizing spray drying is an efficient technique for reducing the bitterness of drugs. Ethylcellulose is a hydrophobic polymer widely used as a taste masking material. Rupatadine fumarate, one of the newest antihistamines, features an intensive bitter taste, hence in designing orodispersible formulations, achieving an acceptable taste is a crucial issue. The main objective of this work was to formulate orodispersible minitablets containing taste masked ethylcellulose-based microparticles with rupatadine fumarate and evaluation of their quality, especially in terms of taste masking efficacy. The accessed data indicated that all obtained minitablets were characterized by beneficial pharmaceutical properties. Three independent methods: in vivo with healthy volunteers, in vitro drug dissolution, and “electronic tongue” confirmed that all designed formulations provided satisfactory taste masking rate and that formulation F15 (prepared with Pearlitol® Flash and Surelease® microparticles with rupatadine fumarate) was characterized by the lowest bitterness score.

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Studying pharmacodynamic effects in cell cultures by chemical fingerprinting − SIA electronic tongue versus 2D fluorescence soft sensor

Cited by 13 publications

References 37 publications

Engineering of 2D Ti3C2 MXene Surface Charge and its Influence on Biological Properties

Engineering of 2D Ti3C2 MXene Surface Charge and its Influence on Biological Properties

Quantum Dots—Assisted 2D Fluorescence for Pattern Based Sensing of Amino Acids, Oligopeptides and Neurotransmitters

Utilization of Ethylcellulose Microparticles with Rupatadine Fumarate in Designing Orodispersible Minitablets with Taste Masking Effect

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