Studying Osteocyte Function Using the Cell Lines MLO-Y4 and MLO-A5

Rosser, Jennifer; Bonewald, Lynda F.

doi:10.1007/978-1-61779-415-5_6

Cited by 49 publications

(52 citation statements)

References 15 publications

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“…The osteocyte cell line MLO-Y4 responded in OM/D—unlike the other osteoblasts—to col/sCS1 with a significant increase of TNAP activity and calcium deposition compared to col-aECM. MLO-Y4 cells reflect the most mature late osteoblast phenotype used in this study [29, 30]. Late osteoblasts/osteocytes derive from active osteoblasts which had synthesized new bone matrix and become incorporated therein [31].…”

Section: Resultsmentioning

confidence: 99%

Artificial Extracellular Matrices with Oversulfated Glycosaminoglycan Derivatives Promote the Differentiation of Osteoblast-Precursor Cells and Premature Osteoblasts

Hempel

Preissler

Vogel

et al. 2014

BioMed Research International

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Sulfated glycosaminoglycans (GAG) are components of the bone marrow stem cell niche and to a minor extent of mature bone tissue with important functions in regulating stem cell lineage commitment and differentiation. We anticipated that artificial extracellular matrices (aECM) composed of collagen I and synthetically oversulfated GAG derivatives affect preferentially the differentiation of osteoblast-precursor cells and early osteoblasts. A set of gradually sulfated chondroitin sulfate and hyaluronan derivatives was used for the preparation of aECM. All these matrices were analysed with human bone marrow stromal cells to identify the most potent aECM and to determine the influence of the degree and position of sulfate groups and the kind of disaccharide units on the osteogenic differentiation. Oversulfated GAG derivatives with a sulfate group at the C-6 position of the N-acetylglycosamine revealed the most pronounced proosteogenic effect as determined by tissue nonspecific alkaline phosphatase activity and calcium deposition. A subset of the aECM was further analysed with different primary osteoblasts and cell lines reflecting different maturation stages to test whether the effect of sulfated GAG derivatives depends on the maturation status of the cells. It was shown that the proosteogenic effect of aECM was most prominent in early osteoblasts.

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Section: Resultsmentioning

confidence: 99%

Artificial Extracellular Matrices with Oversulfated Glycosaminoglycan Derivatives Promote the Differentiation of Osteoblast-Precursor Cells and Premature Osteoblasts

Hempel

Preissler

Vogel

et al. 2014

BioMed Research International

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show abstract

“…BMSCs and pre-osteoblast MC3T3-E1 cells [20, 44] were cultured in α-Modified Eagle’s Medium (α-MEM; Thermo Scientific) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals, Lawrenceville, GA) [43, 48]. Murine osteocyte, MLO-Y4 cells were kindly provided by Lynda F. Bonewald (University of Missouri–Kansas City, MO) and described in [45, 49] and cultured in α-MEM supplemented with 5% FBS and 5% calf serum (Gibco, Life technologies, Grand Island, NY) on collagen coated plates [45]. All growth medium were supplemented with 1% L-glutamine (Invitrogen, Life technologies), and 1% of penicillin/streptomycin (Thermo Scientific).…”

Section: Methodsmentioning

confidence: 99%

Nano-hydroxyapatite modulates osteoblast lineage commitment by stimulation of DNA methylation and regulation of gene expression

et al. 2015

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Hydroxyapatite (HA) is the primary structural component of the skeleton and dentition. Under biological conditions, HA does not occur spontaneously and therefore must be actively synthesized by mineralizing cells such as osteoblasts. The mechanism(s) by which HA is actively synthesized by cells and deposited to create a mineralized matrix are not fully understood and the consequences of mineralization on cell function are even less well understood. HA can be chemically synthesized (HAp) and is therefore currently being investigated as a promising therapeutic biomaterial for use as a functional scaffold and implant coating for skeletal repair and dental applications. Here we investigated the biological effects of nano-HAp (10×100 nm) on the lineage commitment and differentiation of bone forming osteoblasts. Exposure of early stage differentiating osteoblasts resulted in dramatic and sustained changes in gene expression, both increased and decreased, whereas later stage osteoblasts were much less responsive. Analysis of the promoter region one of the most responsive genes, alkaline phosphatase, identified the stimulation of DNA methylation following cell exposure to nano-HAp. Collectively, the results reveal the novel epigenetic regulation of cell function by nano-HAp which has significant implication on lineage determination as well as identifying a novel potential therapeutic use of nanomaterials.

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“…On the other hand, since the composites proposed in the present contribution have been designed to be employed in bone tissue engineering and repair, they were further tested with respect to MLO-Y4, i.e. an osteocite-like immortalized cell line isolated from murine long bones, whose properties are very similar to those of primary osteocytes [67][68][69][70][71]. In recent years osteocytes have been a major focus in the bone field research, since they represent more than 95% of the cellular component of mature adult bone [72,73].…”

Section: Biocompatibility Testsmentioning

confidence: 99%

Bioactive glass/hydroxyapatite composites: Mechanical properties and biological evaluation

Bellucci¹,

Sola²,

Anesi³

et al. 2015

Materials Science and Engineering: C

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Studying Osteocyte Function Using the Cell Lines MLO-Y4 and MLO-A5

Cited by 49 publications

References 15 publications

Artificial Extracellular Matrices with Oversulfated Glycosaminoglycan Derivatives Promote the Differentiation of Osteoblast-Precursor Cells and Premature Osteoblasts

Artificial Extracellular Matrices with Oversulfated Glycosaminoglycan Derivatives Promote the Differentiation of Osteoblast-Precursor Cells and Premature Osteoblasts

Nano-hydroxyapatite modulates osteoblast lineage commitment by stimulation of DNA methylation and regulation of gene expression

Bioactive glass/hydroxyapatite composites: Mechanical properties and biological evaluation

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