Studying on Growth of Strawberry (Fragaria Ananassa L.) Cell Suspension Cultures for Anthocyanin Production

Abstract

Cell suspension cultures were initated from calli derived from in vitro strawberry leaves on MS medium (Murashige and Skoog, 1962) supplemented with 30 g/l sucrose, 1.0 mg/l 2,4-D and 0.3 mg/l kinetin. There were many factors affected on cell suspension cultures growth (it was found that …). Cell suspension cultures grew better on MS medium with 30 g/l sucrose. 1 g (fresh weight) of cells in 20 ml of medium was the best initial inoculum cell density for cell suspension cultures to grow. A shaking speed of 100 rpm on rotary shaker was suitable for the cells. The growth of cell suspension in dark was better than that under light condition. Anthocyanin in the cells was determined by pH differential method.

Rosmarinic acid production in cell suspension cultures of Ehretia asperula Zollinger & Moritz

“…It was showed that cell density affected the growth of strawberry cell suspension (Fragaria ananassa L.), with a 1:20 (w/v) inoculum size being the most effective (17). However, unlike for Panax ginseng suspension cultures, cell growth was low at an inoculum size of 1:25 (w/v) and maximum cell growth was achieved with increasing inoculum size up to 1:10 (w/v).…”