Studying of Phenomenon of Biological Adaptation to Heavy Water

Abstract

Biological influence of deuterium on cells of various taxonomic groups of prokaryotic and eucaryotic microorganisms realizing methylotrophic, chemoheterotrophic, photo-organotrophic, and photosynthetic ways of assimilation of carbon substrates (methylotrophic bacteria Brevibacterium methylicum, chemoheterotrophic bacteria Bacillus subtilis, photo-organotrophic halobacteria Halobacterium halobium, and green micro algae Chlorella vulgaris) was investigated at the growth on media with heavy water ( 2 H2O). For investigated microorganisms are submitted the data on growth and adaptation on the growth media containing as sources of deuterated substrates 2 H2O, [ 2 H]methanol and hydrolisates of deutero-biomass of methylotrophic bacteria B. methylicum, obtained after multistage adaptation to 2 H2O. The qualitative and quantitative composition of intra-and endocellular amino acids, proteins, carbohydrates and fatty acids in conditions of adaptation to 2 H2O is investigated. It is shown, that the effects observed at adaptation to 2 H2O, possess a complex multifactorial character and connected to cytological, morphological and physiological changes -the magnitude of the lag-period, time of cellular generation, output of