Studying Membrane Protein Trafficking in <em>Drosophila</em> Photoreceptor Cells Using eGFP-Tagged Proteins

Methods for Studying the Optics, Physiology, and Biochemistry of the Fly Compound Eye

“…Importantly, there have been many attempts to produce bioinspired artificial eyes, and these have relied on quantitative studies of existing compound eyes.Wagner et al utilize the deep pseudopupil, a unique optical phenomenon of the compound eye, and high amplification microscopy with the optical neutralization of the cornea to localize eGFPtagged proteins within Drosophila photoreceptor cells14 . This method allows the detection of structural defects in the rhabdomeres due to photoreceptor degeneration and cellular localization and trafficking of important signaling proteins.Gutorov et al utilize electrophysiological measurementsfrom the whole eye or from single photoreceptor cells to measure the level of photopigment states in vivo and their modulations by specific mutations in signaling proteins15 .…”