“…In addition, the data are very heterogeneous, making any comparison difficult. Several publications showed no significant difference between sham or exposed cells [Gurisik et al, 2006;Qutob et al, 2006;Whitehead et al, 2006a;Chauhan et al, 2007;Huang et al, 2008], or described weak changes that were not confirmed by RT-PCR validation [Zeng et al, 2006;Paparini et al, 2008;Roux et al, 2011;Sakurai et al, 2011], while other microarray studies reported modified gene expression after radiofrequency exposure [Belyaev et al, 2006;Nylund and Leszczynski, 2006;Remondini et al, 2006;Zhao et al, 2007;Huang et al, 2008]. However, these positive studies reported a small number of responsive genes (9-34) with a FC generally close to, or lower than 2.…”
The main purpose of this study is to investigate potential responses of skin cells to millimeter wave (MMW) radiation increasingly used in the wireless technologies. Primary human skin cells were exposed for 1, 6, or 24 h to 60.4 GHz with an average incident power density of 1.8 mW/cm(2) and an average specific absorption rate of 42.4 W/kg. A large-scale analysis was performed to determine whether these exposures could affect the gene expression. Gene expression microarrays containing over 41,000 unique human transcript probe sets were used, and data obtained for sham and exposed cells were compared. No significant difference in gene expression was observed when gene expression values were subjected to a stringent statistical analysis such as the Benjamini-Hochberg procedure. However, when a t-test was employed to analyze microarray data, 130 transcripts were found to be potentially modulated after exposure. To further quantitatively analyze these preselected transcripts, real-time PCR was performed on 24 genes with the best combination of high fold change and low P-value. Five of them, namely CRIP2, PLXND1, PTX3, SERPINF1, and TRPV2, were confirmed as differentially expressed after 6 h of exposure. To the best of our knowledge, this is the first large-scale study reporting on potential gene expression modification associated with MMW radiation used in wireless communication applications.
“…In addition, the data are very heterogeneous, making any comparison difficult. Several publications showed no significant difference between sham or exposed cells [Gurisik et al, 2006;Qutob et al, 2006;Whitehead et al, 2006a;Chauhan et al, 2007;Huang et al, 2008], or described weak changes that were not confirmed by RT-PCR validation [Zeng et al, 2006;Paparini et al, 2008;Roux et al, 2011;Sakurai et al, 2011], while other microarray studies reported modified gene expression after radiofrequency exposure [Belyaev et al, 2006;Nylund and Leszczynski, 2006;Remondini et al, 2006;Zhao et al, 2007;Huang et al, 2008]. However, these positive studies reported a small number of responsive genes (9-34) with a FC generally close to, or lower than 2.…”
The main purpose of this study is to investigate potential responses of skin cells to millimeter wave (MMW) radiation increasingly used in the wireless technologies. Primary human skin cells were exposed for 1, 6, or 24 h to 60.4 GHz with an average incident power density of 1.8 mW/cm(2) and an average specific absorption rate of 42.4 W/kg. A large-scale analysis was performed to determine whether these exposures could affect the gene expression. Gene expression microarrays containing over 41,000 unique human transcript probe sets were used, and data obtained for sham and exposed cells were compared. No significant difference in gene expression was observed when gene expression values were subjected to a stringent statistical analysis such as the Benjamini-Hochberg procedure. However, when a t-test was employed to analyze microarray data, 130 transcripts were found to be potentially modulated after exposure. To further quantitatively analyze these preselected transcripts, real-time PCR was performed on 24 genes with the best combination of high fold change and low P-value. Five of them, namely CRIP2, PLXND1, PTX3, SERPINF1, and TRPV2, were confirmed as differentially expressed after 6 h of exposure. To the best of our knowledge, this is the first large-scale study reporting on potential gene expression modification associated with MMW radiation used in wireless communication applications.
“…Methods based on array technologies have recently shown significant modulation of gene expression levels after exposing human endothelial cells Huang et al, 2008] or murine cells [Zhao et al, 2007] to HF EMF. However, similar studies have shown no evidence that such effects occurred [Zeng et al, 2006;Chauhan et al, 2007], illustrating that this research area still suffers from contradictory conclusions and needs to be investigated more carefully.…”
We exposed normal human epidermal keratinocytes to short duration, high frequency, and low amplitude electromagnetic fields, similar to that used by mobile phone technologies. We paid particular attention to the control of the characteristics of the electromagnetic environment generated within a mode stirred reverberation chamber (statistical homogeneity and isotropy of the field and SAR distribution). Two non-thermal exposure conditions were tested on the epidermal cells: 10-min exposure with a field amplitude of 8 V/m, and 30 min with 41 V/m. Corresponding specific absorption rates ranged from 2.6 to 73 mW/kg (continuous wave, 900 MHz carrier frequency). We collected RNA from cells subjected to these conditions and used it for a large-scale microarray screening of over 47000 human genes. Under these conditions, exposure of keratinocytes to the electromagnetic field had little effect; only 20 genes displayed significant modulation. The expression ratios were very small (close to 1.5-fold change), and none of them were shared by the two tested conditions. Furthermore, those assayed using polymerase chain reaction did not display significant expression modulation (overall mean of the exposed samples: 1.20 ± 0.18). In conclusion, the data presented here show that cultured keratinocytes are not significantly affected by EMF exposure.
“…1,800 MHz RF radiation at 2 W/kg got 24 h in rat neuron altered expression of cytoskeleton genes such as microtubule-associated protein 2 (MAP2) and signal transduction-related genes as well as immediate early gene (EGR1) (Zhao et al, 2007). Recently, it has been…”
Radiofrequency (RF) radiation might induce the transcription of a certain set of genes as other physical stresses like ionizing radiation and UV. To observe transcriptional changes upon RF radiation, we exposed WI-38, human lung fibroblast cell to 1763 MHz of mobile phone RF radiation at 60 W/kg of specific absorption rate (SAR) for 24h with or without heat control. There were no significant changes in cell numbers and morphology after exposure to RF radiation. Using quantitative RT-PCR, we checked the expression of three heat shock protein (HSP) (HSPA1A, HSPA6 and HSP105) and seven stress-related genes (TNFRSF11B, FGF2, TGFB2, ITGA2, BRIP1, EXO1, and MCM10) in RF only and RF/HS groups of RF-exposed cells. The expressions of three heat shock proteins and seven stress-related genes were selectively changed only in RF/HS groups. Based on the expression of ten genes, we could classify thermal and non-thermal effect of RF-exposure, which genes can be used as biomarkers for RF radiation exposure.
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