2019
DOI: 10.1007/978-1-0716-0211-9_1
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Studying Evolutionary Adaptation of MERS-CoV

Abstract: Forced viral adaptation is a powerful technique employed to study the ways viruses may overcome various selective pressures that reduce viral replication. Here, we describe methods for in vitro serial passaging of Middle East respiratory syndrome coronavirus (MERS-CoV) to select for mutations which increase replication on semi-permissive cell lines as described in Letko et al., Cell Rep 24, 1730-1737, 2018

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“…Coronaviruses are a large family of respiratory viruses. Several viral epidemics have occurred in the previous two decades, including the severe acute respiratory syndrome coronavirus (SARS-CoV) in 2002-2003 and H1N1 influenza in 2009. In 2012, the Middle East respiratory syndrome coronavirus (MERS-CoV) was detected for the first time in Saudi Arabia (Letko and Munster, 2020). Originally, these viruses were passed from animals to humans.…”
Section: Introductionmentioning
confidence: 99%
“…Coronaviruses are a large family of respiratory viruses. Several viral epidemics have occurred in the previous two decades, including the severe acute respiratory syndrome coronavirus (SARS-CoV) in 2002-2003 and H1N1 influenza in 2009. In 2012, the Middle East respiratory syndrome coronavirus (MERS-CoV) was detected for the first time in Saudi Arabia (Letko and Munster, 2020). Originally, these viruses were passed from animals to humans.…”
Section: Introductionmentioning
confidence: 99%