Studying circadian rhythms in Drosophila melanogaster

Tataroglu, Ozgur; Emery, Patrick

doi:10.1016/j.ymeth.2014.01.001

Cited by 84 publications

(68 citation statements)

References 177 publications

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“…Historically, Drosophila has been at the forefront of circadian rhythm research due to its ease of use and its advanced molecular and genetic toolkit [3]. Importantly, the molecular circadian clock is highly conserved from Drosophila to humans and consists of interlocked transcriptional negative feedback loops (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

The molecular ticks of the Drosophila circadian clock

Tataroglu

Emery

2015

Current Opinion in Insect Science

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108

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Drosophila is a powerful model to understand the mechanisms underlying circadian rhythms. The Drosophila molecular clock is comprised of transcriptional feedback loops. The expressions of the critical transcriptional activator CLK and its repressors PER and TIM are under tight transcriptional control. However, posttranslational modification of these proteins and regulation of their stability are critical to their function and to the generation of 24-hr period rhythms. We review here recent progress made in our understanding of PER, TIM and CLK posttranslational control. We also review recent studies that are uncovering the importance of novel regulatory mechanisms that affect mRNA stability and translation of circadian pacemaker proteins and their output.

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Section: Introductionmentioning

confidence: 99%

The molecular ticks of the Drosophila circadian clock

Tataroglu

Emery

2015

Current Opinion in Insect Science

Self Cite

108

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“…These two daily activity bouts are often referred to as the morning (M) and evening (E) activity peaks (3)(4)(5). Flies generally take a midday siesta between these two activity peaks, and they experience consolidated nighttime sleep for most of the 12 h of darkness after lights-off (6).…”

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confidence: 99%

“…This circuitry comprises approximately 75 pairs of neurons that express the core circadian proteins. Clock neurons can be further divided into several groups based on their anatomic position within the adult brain (6). The ventral lateral neurons (LNvs) consist of small and large cells (s-LNvs and l-LNvs, respectively).…”

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confidence: 99%

Temporal calcium profiling of specific circadian neurons in freely moving flies

Guo

Xiao

Rosbash

2017

Proc. Natl. Acad. Sci. U.S.A.

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There are no general methods for reliably assessing the firing properties or even calcium profiles of specific neurons in freely moving flies. To this end, we adapted a GFP-based calcium reporter to luciferase that was expressed in small subsets of circadian neurons. This Tric-LUC reporter allowed a direct comparison of luciferase activity with locomotor activity, which was assayed in the same flies with video recording. The LUC profile from activitypromoting E cells paralleled evening locomotor activity, and the LUC profile from sleep-promoting glutamatergic DN1s (gDN1s) paralleled daytime sleep. Similar profiles were generated by novel reporters recently identified based on transcription factor activation. As E cell and gDN1 activity is necessary and sufficient for normal evening locomotor activity and daytime sleep profiles, respectively, we suggest that their luciferase profiles reflect their neuronal calcium and in some cases firing profiles in wake-behaving flies.circadian | sleep | Drosophila | optogenetics | calcium

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“…Collectively, these different tools and approaches can be used to study the rich set of innate behaviors performed by flies such as walking, flight, grooming, feeding, mating, fighting, and escape. Moreover, behavioral and circuit-based studies in flies have provided new insights into basic topics in neuroscience such as learning and memory, sensory-motor integration, neuromodulation, sleep, behavioral choice, behavioral sequencing, motor control, and sensory systems (Huston and Pavlou and Goodwin 2013;Wilson 2013;Tataroglu and Emery 2014;Borst 2014;Borgmann and Büschges 2015;Owald et al 2015;Hoopfer 2016;Masek and Keene 2016;McKellar 2016). Given that many of the tools used to study neural circuits have only recently become available, we anticipate that the coming years will experience a rapid growth in our understanding of how neural circuits within the fruit fly nervous system are organized to produce particular behaviors.…”

Section: Introductionmentioning

confidence: 99%

Targeted manipulation of neuronal activity in behaving adult flies

Hampel

Seeds

2016

Preprint

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The ability to control the activity of specific neurons in freely behaving animals provides an effective way to probe the contributions of neural circuits to behavior. Wide interest in studying principles of neural circuit function using the fruit fly Drosophila melanogaster has fueled the construction of an extensive transgenic toolkit for performing such neural manipulations. Here we describe approaches for using these tools to manipulate the activity of specific neurons and assess how those manipulations impact the behavior of flies. We also describe methods for examining connectivity among multiple neurons that together form a neural circuit controlling a specific behavior. This work provides a resource ABSTRACTThe ability to control the activity of specific neurons in freely behaving animals provides an effective way to probe the contributions of neural circuits to behavior. Wide interest in studying principles of neural circuit function using the fruit fly Drosophila melanogaster has fueled the construction of an extensive transgenic toolkit for performing such neural manipulations. Here we describe approaches for using these tools to manipulate the activity of specific neurons and assess how those manipulations impact the behavior of flies. We also describe methods for examining connectivity among multiple neurons that together form a neural circuit controlling a specific behavior. This work provides a resource for researchers interested in examining how neurons and neural circuits contribute to the rich repertoire of behaviors performed by flies.

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Studying circadian rhythms in Drosophila melanogaster

Cited by 84 publications

References 177 publications

The molecular ticks of the Drosophila circadian clock

The molecular ticks of the Drosophila circadian clock

Temporal calcium profiling of specific circadian neurons in freely moving flies

Targeted manipulation of neuronal activity in behaving adult flies

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