Studying bone marrow-derived macrophages needs to consider the influence of cells apart from macrophage, especially the selection of markers

Abstract

Culturing macrophage in vitro is an important means to understand its reaction towards co-culture substances. However, the source of macrophages can be either purchased from specific cell line or extracted from bone marrow as differentiated macrophages. In order to assure the precision, when adopting extracted primary cell, screening in advance will be prominent before any processes to avoid results reserving that of non-macrophages. Here, we perform single-cell sequencing on open wound of skin in mice and focusing on the secreted proteins and surface markers related to traditional macrophage phenotypes (M1/M2) to ensure the importance of screening. The expression of CAMP when macrophages fight against candida albicans is another target to see its relationship with current classification. And results showed that identifying its phenotype without screening macrophages will far from the exact situation, and the expression of CAMP cannot be carried out by the traditional M1 and M2 macrophage. Thus, determining phenotype of macrophages based on function would be a promising way.