Studying Arabidopsis Chloroplast Structural Organisation Using Transmission Electron Microscopy

Hyman, Stefan C.; Jarvis, Paul

doi:10.1007/978-1-61779-234-2_8

Cited by 14 publications

(9 citation statements)

References 17 publications

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“…The grids were viewed under a JEOL JEM-1400 electron microscope at 80 kV. Digital images were recorded using a SIS digital camera and iTEM software (Glauert and Lewis, 1998;Hyman and Jarvis, 2011).…”

Section: Transmission Electron Microscopymentioning

confidence: 99%

Suppressors of the Chloroplast Protein Import Mutant tic40 Reveal a Genetic Link between Protein Import and Thylakoid Biogenesis

Bédard

Trösch

et al. 2017

Plant Cell

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To extend our understanding of chloroplast protein import and the role played by the import machinery component Tic40, we performed a genetic screen for suppressors of chlorotic tic40 knockout mutant Arabidopsis thaliana plants. As a result, two suppressor of tic40 loci, stic1 and stic2, were identified and characterized. The stic1 locus corresponds to the gene ALBINO4 (ALB4), which encodes a paralog of the well-known thylakoid protein targeting factor ALB3. The stic2 locus identified a previously unknown stromal protein that interacts physically with both ALB4 and ALB3. Genetic studies showed that ALB4 and STIC2 act together in a common pathway that also involves cpSRP54 and cpFtsY. Thus, we conclude that ALB4 and STIC2 both participate in thylakoid protein targeting, potentially for a specific subset of thylakoidal proteins, and that this targeting pathway becomes disadvantageous to the plant in the absence of Tic40. As the stic1 and stic2 mutants both suppressed tic40 specifically (other TIC-related mutants were not suppressed), we hypothesize that Tic40 is a multifunctional protein that, in addition to its originally described role in protein import, is able to influence downstream processes leading to thylakoid biogenesis.

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Section: Transmission Electron Microscopymentioning

confidence: 99%

Suppressors of the Chloroplast Protein Import Mutant tic40 Reveal a Genetic Link between Protein Import and Thylakoid Biogenesis

Bédard

Trösch

et al. 2017

Plant Cell

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show abstract

“…The grids were viewed under a JEOL JEM-1400 electron microscope at 80 kV. Digital images were recorded using a SIS digital camera and iTEM software (Olympus; Glauert, 1998;Hyman and Jarvis, 2011).…”

Section: Total Chlorophyll and Carotenoid Measurements Transmission mentioning

confidence: 99%

Genetic and Physical Interaction Studies Reveal Functional Similarities between ALBINO3 and ALBINO4 in Arabidopsis

et al. 2015

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ALBINO3 (ALB3) is a well-known component of a thylakoid protein-targeting complex that interacts with the chloroplast signal recognition particle (cpSRP) and the cpSRP receptor, chloroplast filamentous temperature-sensitive Y (cpFtsY). Its proteininserting function has been established mainly for light-harvesting complex proteins, which first interact with the unique chloroplast cpSRP43 component and then are delivered to the ALB3 integrase by a GTP-dependent cpSRP-cpFtsY interaction. In Arabidopsis (Arabidopsis thaliana), a subsequently discovered ALB3 homolog, ALB4, has been proposed to be involved not in light-harvesting complex protein targeting, but instead in the stabilization of the ATP synthase complex. Here, however, we show that ALB3 and ALB4 share significant functional overlap, and that both proteins are required for the efficient insertion of cytochrome f and potentially other subunits of pigment-bearing protein complexes. Genetic and physical interactions between ALB4 and ALB3, and physical interactions between ALB4 and cpSRP, suggest that the two ALB proteins may engage similar sets of interactors for their specific functions. We propose that ALB4 optimizes the insertion of thylakoid proteins by participating in the ALB3-cpSRP pathway for certain substrates (e.g. cytochrome f and the Rieske protein). Although ALB4 has clearly diverged from ALB3 in relation to the partner-recruiting C-terminal domain, our analysis suggests that one putative cpSRP-binding motif has not been entirely lost.

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“…For transmission electron microscopy (TEM), seeds were fixed and embedded according to Hyman and Jarvis (2011) . Sections of ~90nm thickness were cut on a Reichert-Jung Ultracut E ultramicrotome and stained with 2% Uranyl acetate (Ajax Chemicals) for 10min and Reynolds lead citrate (BDH Ltd.) for 8min.…”

Section: Methodsmentioning

confidence: 99%

Developmentally regulatedHEART STOPPER, a mitochondrially targeted L18 ribosomal protein gene, is required for cell division, differentiation, and seed development inArabidopsis

Zhang

Luo

Day

et al. 2015

EXBOTJ

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Studying Arabidopsis Chloroplast Structural Organisation Using Transmission Electron Microscopy

Cited by 14 publications

References 17 publications

Suppressors of the Chloroplast Protein Import Mutant tic40 Reveal a Genetic Link between Protein Import and Thylakoid Biogenesis

Suppressors of the Chloroplast Protein Import Mutant tic40 Reveal a Genetic Link between Protein Import and Thylakoid Biogenesis

Genetic and Physical Interaction Studies Reveal Functional Similarities between ALBINO3 and ALBINO4 in Arabidopsis

Developmentally regulatedHEART STOPPER, a mitochondrially targeted L18 ribosomal protein gene, is required for cell division, differentiation, and seed development inArabidopsis

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