Study to Identify the Signalling Cascade behind Expression Level of PTEN and RB1 gene in Breast Cancer

Abstract

Breast cancer is the second most prevalent cancer among Pakistani women and the disease burden of breast cancer is continuously uprising. There are several genes involved in breast cancer incidence. The current study was designed to analyze the gene expression level of hereditary onco-suppressive PTEN and RB1, proto-oncogenes i.e. Src and KRAS, and microRNA-140/145/238in breast cancer patients performed through qRT-PCR.Results showed significant up-regulation of proto-oncoSrc and KRAS genes (p<0.05). On the other hand, a significant down-regulation of hereditary onco-suppressive PTEN and RB1 genes while as, the micro RNA signalling cascade involvement through higher expression levels of MI-140, Mi-145, and Mi-238 was also observed (p<0.05). Biopsy samples were preserved in 10% formalin for histopathology as well as in Trizol for mRNA extraction. Histopathlogical examination showed multilayering, hyperplasia, and a complete distortion of ductal and glandular epithelium of breast gland in breast cancer patients.