2010
DOI: 10.32894/kujss.2010.41128
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Study The Radiatory Pollution in Selected Soils of Kirkuk Governorate

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Cited by 2 publications
(2 citation statements)
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“…For the detection of H. pylori DNA presence in the blood, DNA of blood samples were amplified by H. pylori specific 16s rDNA (or 16s rRNA) gene, sequence of primers was: C-97, 5'-GCT ATG ACG GGT ATC C-3' (276-291 forward), C-98, 5'-GAT TTT ACC CCT ACA CCA -3' (681-698 reverse), and the product size was ~ 400 base-pair (45) (24) (22) (13). Singleplex PCR program was as followed: initial-denaturation 94 ˚C and final extension 72 ˚C were for five minutes and 1 cycle for each, while denaturation, annealing, and extension were for only one minute but 35 cycles and the temperature was (94, 50, 72) ˚C respectively.…”
Section: Molecular Diagnosis Of H Pylori By Pcrmentioning
confidence: 99%
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“…For the detection of H. pylori DNA presence in the blood, DNA of blood samples were amplified by H. pylori specific 16s rDNA (or 16s rRNA) gene, sequence of primers was: C-97, 5'-GCT ATG ACG GGT ATC C-3' (276-291 forward), C-98, 5'-GAT TTT ACC CCT ACA CCA -3' (681-698 reverse), and the product size was ~ 400 base-pair (45) (24) (22) (13). Singleplex PCR program was as followed: initial-denaturation 94 ˚C and final extension 72 ˚C were for five minutes and 1 cycle for each, while denaturation, annealing, and extension were for only one minute but 35 cycles and the temperature was (94, 50, 72) ˚C respectively.…”
Section: Molecular Diagnosis Of H Pylori By Pcrmentioning
confidence: 99%
“…(21). AL-Jobori et al, when using the PCR for detection 16S rRNA gene which was specific for H. pylori, reported that 20 (100 %) of patients were positive for PCR from peripheral blood (13). The study result was in agreement with saeideh et al, who revealed that H. pylori can secrete DNA in peripheral blood besides its existence in gastric mucosa, and it is possible for the infection of H. pylori to cause bacteremia or inject DNA to blood by type IV secretory system.…”
Section: Figure 1 Gel Electrophoresis Of Genomic Dna Of Blood Samplesmentioning
confidence: 99%