Study the Effects of Follicular Size on some Biochemical Follicular Fluid Composition in She Camel (Camelus Dromedarius)

Abstract

| This study was designed to estimate the biochemical composition of ovary follicular fluid in relation to its size variations in local Iraqi She camels (Camelus dromedarius). Hundred ovary were collected from 50 adult she camel, 4 to 10 years old that slaughtered at Al Najaf abattoir during the breeding season of camelids. The ovaries were transferred immediately to the laboratory in a cold box. Later on, the follicular fluid was collected separately from small and large size follicles, (3-9 mm) and (10-19 mm) respectively. The fluid was kept at -4 0 C for further analysis. The follicular fluid samples were analyzed to estimate the metabolic composition (cholesterol, glucose and total protein), and the ionic compositions (calcium, sodium and potassium). The results of this study revealed significant (P< 0.05) increase inthe glucose and cholesterol concentration, while there were significant decrease in the total protein in large size follicles. Meanwhile, significant increase was seen in the concentration of Na+ and Ca+2 in relation to the size of the follicles. However, significant decrease was occurred in the concentrations of K with increasing follicle size. In conclusion, the results of this study revealed a significant variations in the concentration of the follicular fluid metabolic and the ionic compositions with the variations of its size.

Biochemical Compositions in the Follicular Fluid of Different Follicle Groups and Their Relationship with the Blood Concentrations in Dromedary Camels

“…In our study, we observed that glucose concentration in follicular fluid was lower in all follicular sizes than those measured in blood serum, indicating ovary is one of the main sites for glucose metabolism in camel. Hassan et al, (2018) stated that large follicles had more glucose concentration than small follicle which is in agreement of our findings. In contrast to our study, Leroy et al (2004) showed in cows that the glucose concentration increases with the pace of increasing the follicle diameter.…”

Reproductive Consequences of Electrolyte Disturbances in Domestic Animals

“…Calcium is an important electrolyte in reproduction. It plays an important role in the production of developing follicle hormones, as well as in the regulation of the secretion of hormones necessary for ovaries and ovulation [ 56 ]. Calcium ions are also important in estrogen synthesis, the levels of which increase during follicle development, necessitating the accumulation of Ca 2+ in the follicular fluid.…”

“…Sodium is associated with follicle viability and its activity in estrogen synthesis. In studies carried out on the ovaries of 50 camels, significant differences ( p < 0.05) were found between the size of the follicle and the concentration of Na + and K + [ 56 ]. In small follicles, 3–9 mm in size, the sodium concentration was 93.33 ± 4.75 mmol/L, while in large follicles (10–19 mm)—145.96 ± 4.26 mmol/L.…”

“…In small follicles, it was 12.96 ± 0.68 mmol/L, and in large follicles, it decreased and amounted to 6.12 ± 0.57 mmol/L. The reduction in the content of these ions is associated with the development of the follicle [ 56 ]. The decrease in potassium concentration is associated with the development of the follicle, which leads to the transfer of potassium ions from the extracellular space to the intracellular space, and thus reduces its concentration in the follicular fluid as the follicle becomes larger.…”

“…In general, this study validated the variability in the concentration of metabolic components and ionic follicular fluids depending on the size of the follicle and its stage of development. The results of this study can be considered [ 56 ]. Potassium channels are found in the ovary and the myometrium of the uterus, which is involved in the secretion of progesterone.…”