Background and objective: The aim of recent study was to find out the serologicalprevalence of toxoplasmosis, as well as the effect of T. gondii on biochemical parameters of womenvisiting Bahawal Victoria Hospital, Bahawalpur.Method: The blood samples were obtained from the women visiting hospital and serum was stored.The Enzyme-linked Immunosorbent Assay measured the immunoglobulin G (IgG) antibodies of T.gondii.Result: The overall prevalence of toxoplasmosis was 58.73%. age wise seroprevalence shows in agegroups (years) 16-25, 26-33, 26-33, ≥42 was (50%), (63%), (55.55%) and (70%) respectively. Therelationship between hosts disease and prevalence of toxoplasmosis in women suffering hepatitis B,kidney problem, arthritis, diabetes, nausea and thyroid abnormality, the parasitic prevalence wasobserved (57.57%), (33.33%), (50%), (57.14%), (75%), and (85.71%) respectively. The relationshipbetween mother having total number of child 0-2, 3-5, ≥5 and prevalence of parasite was (62.96%),(60%), (33.33%) respectively. The prevalence of parasite in women belong different socio economicstatus poor, middle, rich and was (94.44%), (46.15%), (33.33%). There was significant differencebetween the parasitic prevalence and the host disease as well as socio economic status. TheMean±SEM biochemical parameters in age (years), disease, socio economic status, number of child ofwomen was comparatively higher in infected hosts as compared to non-infected hosts.Conclusion: In conclusions the findings indicated there is a noticeable prevalence of T. gondii so thatcontrol and serological screening programs should be implemented in Bahawalpur for the reliable andfast detection of Toxoplasma infections.