Study the Anti-Inflammatory Effects of Tamsulosin by the Evaluation of Inflammatory Cells and Lung Histopathology in an Airway Inflammation Model in Rats

Abstract

Objective: Study the role of tamsulosin on the respiratory inflammation in rats with ovalbumin (OVA) induced airway sensitization by evaluating the inflammatory cells in the broncho-alveolar lavage fluid (BALF) and the lung histopathology. Materials and methods: Thirty adult male albino rats were allocated into 5 groups (n=6). Group A -Normal control (NC) fed commercial pellets and water. Group B -(as negative control)subjected to an airway OVA-sensitization. Group C (as positive control)treated with oral prednisolone (4.12 mg/kg) plus OVA-sensitization. Group Dtreated with oral tamsulosin (35 mcg/kg/d, equivalent to 0.4 mg for a 70 kg human) plus OVA-sensitization. Group Etreated with oral tamsulosin (17.5 mcg/kg/d, equivalent to 0.2 mg tamsulosin for a 70 kg human) plus OVAsensitization. Inflammatory cells count/µl in the BALF was calculated along with histological analysis of the lung tissue. Results: Both doses of tamsulosin (35 and 17.5 mcg/kg/d) significantly reduced the total WBC count, eosinophils, and neutrophils. A significant reduction in mononuclear cells was detected after treatment with 35 mcg/kg/d tamsulosin. Also, the histopathological examination revealed that both doses (35 and 17.5 mcg/kg/d) of tamsulosin caused less agglomeration of the inflammatory cells within the lung tissue and clear alveolar sacs. Conclusion: the administration of tamsulosin in rats with induced airway sensitization resulted in protection from respiratory inflammatory events.

An Investigation of Anti-inflammatory Effects of Famotidine in Rats by Evaluating Inflammatory Cells and Lung Histopathology: An Airway Model

“…The rats inhaled this aerosol for 30 minutes each day as part of the challenging process. On day 15, the rats were killed by an IP injection of (800 mg/kg) of sodium phenobarbital and sacrificed for further analysis and examination (Ahmed et al, 2021, Algaem et al, 2013, Alabdali and Ibrahim, 2023. In groups receiving prednisolone and famotidine, the drug doses were given 60 minutes before exposing the rats to airway sensitization with OVA.…”

“…The broncho-alveolar lavage fluid (BALF) was then centrifuged at 3000 rpm for 4 min. The inflammatory cells present in the BALF were then quantified using an automated hematology analyzer (Alabdali and Ibrahim, 2023, Poitout-Belissent et al, 2021, Alabdali and Algaem, 2023.…”