Study on urine metabolic profiling and pathogenesis of hyperlipidemia

Liu, Yang; Zhu, Li; Yan-qi, Song; Liu, Yijia; Zhao, Huan; Liu, Yuechen; Zhang, Tianpu; Yuan, Yu; Cai, Xuemeng; Wang, Shuo; Wang, Pengwei; Gao, Shan; Li, Lin; Li, Yubo; Yu, Chunquan

doi:10.1016/j.cca.2019.05.001

Cited by 42 publications

(25 citation statements)

References 34 publications

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“…Supporting our findings, levels of urinary lysine and tyramine levels show lower levels in patients with the metabolic syndrome patients (aged between 24–72 years) and correlate negatively with cardiometabolic features and inflammatory biomarkers [ 45 , 46 ]. Also, decreased levels of glutamine in urine have been identified as a hyperlipidemia biomarker (aged 25–65 years) [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Urinary metabolic signatures reflect cardiovascular risk in the young, middle-aged, and elderly populations

et al. 2020

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The predictive value of traditional cardiovascular risk estimators is limited, and young and elderly populations are particularly underrepresented. We aimed to investigate the urine metabolome and its association with cardiovascular risk to identify novel markers that might complement current estimators based on age. Urine samples were collected from 234 subjects categorized into three age-grouped cohorts: 30–50 years (cohort I, young), 50–70 years (cohort II, middle-aged), and > 70 years (cohort III, elderly). Each cohort was further classified into three groups: (a) control, (b) individuals with cardiovascular risk factors, and (c) those who had a previous cardiovascular event. Novel urinary metabolites linked to cardiovascular risk were identified by nuclear magnetic resonance in cohort I and then evaluated by target mass spectrometry quantification in all cohorts. A previously identified metabolic fingerprint associated with atherosclerosis was also analyzed and its potential risk estimation investigated in the three aged cohorts. Three different metabolic signatures were identified according to age: 2-hydroxybutyrate, gamma-aminobutyric acid, hypoxanthine, guanidoacetate, oxaloacetate, and serine in young adults; citrate, cyclohexanol, glutamine, lysine, pantothenate, pipecolate, threonine, and tyramine shared by middle-aged and elderly adults; and trimethylamine N-oxide and glucuronate associated with cardiovascular risk in all three cohorts. The urinary metabolome contains a metabolic signature of cardiovascular risk that differs across age groups. These signatures might serve to complement existing algorithms and improve the accuracy of cardiovascular risk prediction for personalized prevention. Key messages • Cardiovascular risk in the young and elderly is underestimated. • The urinary metabolome reflects cardiovascular risk across all age groups. • Six metabolites constitute a metabolic signature of cardiovascular risk in young adults. • Middle-aged and elderly adults share a cardiovascular risk metabolic signature. • TMAO and glucuronate levels reflect cardiovascular risk across all age groups.

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Section: Discussionmentioning

confidence: 99%

Urinary metabolic signatures reflect cardiovascular risk in the young, middle-aged, and elderly populations

et al. 2020

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“…Most acute cardiovascular events are attributed to vulnerable carotid atherosclerotic plaque rupture and secondary thrombosis [15][16] . Dyslipidemia is the main risk factor for atherosclerosis [17][18] . LDL-C is considered a major cardiovascular risk factor, with a denser reduction in LDL-C more associated with reduced total and cardiovascular mortality in patients at baseline [19] .…”

Section: Discussionmentioning

confidence: 99%

LDL-C/HDL-C and carotid plaques in patients with coronary heart disease: A Chinese cohort study

Cheng

Yi-jia

et al. 2021

Preprint

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Background: Evidence of the association the low-/high-density lipoprotein cholesterol ratio (LDL-C/HDL-C) with the carotid plaques remains limited. The purpose of this study is to examine the association between LDL-C/HDL-C and carotid plaques of coronary heart disease (CHD), and to study what extent a healthy lifestyle reduces the risk of carotid plaques.Methods: In this large-scale and multi-center retrospective study, a total of 9,426 participants to explore the association between LDL-C/HDL-C and carotid plaques. No smoking and no drinking were considered healthy lifestyle. Generalised estimating equation models and conditional logistic regressions were used in statistical analyses.Results: In all the 9,426 participants, there were 6,989 (74.15%) patients having identified carotid plaques. High levels of LDL-C/HDL-C had a higher risk of carotid plaques than other lipid variables (OR:1.63; 95%CI:1.43-1.86). In stratified analyses by LDL-C/HDL-C triplet, participants in the LDL-C/HDL-C (＞3mmol/L) group had a higher risk of carotid plaques compared to other two groups. Compared with the unfavourable lifestyle, intermediate lifestyle or favourable lifestyle was associated with a significant 30% or 67% decrease in carotid plaques risk among patients with the LDL-C/HDL-C(＞3mmol/L) respectively. There were significantly additive and multiplicative interactions between lifestyle and LDL-C/HDL-C on carotid plaques.Conclusion: Our findings provide evidence that a high level of LDL-C/HDL-C can increase the risk of carotid plaques in patients with CHD. And adhering to a healthy lifestyle has additive beneficial effects on reducing the risk of carotid plaques.

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“…The altered lipoprotein metabolism involving low-density lipoprotein (LDL), high-density lipoprotein (HDL), and triglycerides (TG) is termed as dyslipidemia (Kolovou et al, 2005). Elevation of plasma cholesterol specifically LDL coupled with a decrease in HDL are the predisposing factors to atherosclerosis and other cardiovascular-related diseases (Yang et al, 2019). Atherosclerosis is a pathological condition whereby the normal structure of the arterial intima becomes asymmetrically narrowed as a result of atherosclerotic plaques formation (Wang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Hyperlipidemia is associated with the elevation of low-density lipoprotein and reduction in circulating high-density lipoprotein (Yang et al, 2019). Hyperlipidemia is a progressive predisposing risk factor to atherosclerosis and coronary heart disease (Maejima et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Stevia: limiting cholesterol synthesis in Hep-G2 cells

Ilias

Hamzah

Ismail

et al. 2020

APJMBB

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As of today, no literature has been reported on the efficacy of stevia on lipid regulations conducted in vitro. Thus, the current study was focusing on the potential of Stevia rebaudiana bertoni as an anti-hypercholesterolemia substitute in limiting the de novo cholesterol synthesis in Hep-G2 cell line. The cytotoxicity and lipid internalization effects of stevia on Hep-G2 cells were assessed quantitatively and qualitatively in this study. As evaluated by MTT assay, commercialized stevia (0.5-20.0 mg/ml) and stevioside (1.0-10 µM) inhibited Hep-G2 cells viability in a dose-dependent manner for 24 hours. IC50 was detected at 8.68 mg/ml (commercialized stevia) and 10.91 µM (stevioside). From the assay, suitable concentrations were chosen to study the effect of stevia on cholesterol internalization in Hep-G2 cells supplemented with exogenous lipids. Cholesterol quantification assay revealed that high concentration commercialized stevia and stevioside promoted significant cholesterol internalized in Hep-G2 cells as compared to simvastatin. Finally, immunofluorescent microscopy assessment was done to qualitatively observe the formation of lipid droplets and low-density lipoprotein receptor in relation to total cholesterol extracted. The microphotographs of immunofluorescent microscopy were in parallel to results obtained from the cholesterol quantification assay which further revealed the effect of stevia as a potential anti-hypercholesterolemia agent.

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Study on urine metabolic profiling and pathogenesis of hyperlipidemia

Cited by 42 publications

References 34 publications

Urinary metabolic signatures reflect cardiovascular risk in the young, middle-aged, and elderly populations

Urinary metabolic signatures reflect cardiovascular risk in the young, middle-aged, and elderly populations

LDL-C/HDL-C and carotid plaques in patients with coronary heart disease: A Chinese cohort study

Stevia: limiting cholesterol synthesis in Hep-G2 cells

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